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DNeasy PowerMax Soil Kit

For the isolation of microbial DNA from large quantities of soil with low microbial load
  • Short isolation time of highly pure DNA from up to 10 g soil in 30 minutes
  • High-quality DNA recovery from low biomass samples from large sample quantities
  • Efficient elimination of humic substances and PCR inhibitors using Inhibitor Removal Technology
  • High-quality DNA that is ready to use in downstream applications    
Biodensity of a soil or other environmental sample is not a problem when using the DNeasy PowerMax Soil Kit. This kit, designed for processing large sample quantities, will give researchers DNA samples free from PCR inhibiting compounds generally associated with soil DNA. Humic substances and the color from compost, manure and sediment are eliminated through Inhibitor Removal Technology. Resulting DNA is ready to use in a variety of downstream applications such as qPCR, Sanger sequencing and next-generation sequencing.

DNeasy PowerMax Soil Kit was formerly sold by MO BIO as PowerMax Soil DNA Isolation Kit.
Cat No./ID: 12988-10
DNeasy PowerMax Soil Kit (10)
302,00 €
Order Product
For the isolation of microbial DNA from large quantities of soil with low microbial load
The DNeasy PowerMax Soil Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
0
Figure 1.
Total genomic DNA was isolated from 8 different soil samples using the DNeasy PowerMax Soil Kit. 0.75 µg of each isolated DNA was analyzed on 1 % TAE agarose gel and ethidium stained. M = Marker DNA. Soil types are identified in the attached table.

Sample Lane Type Amount Processed (g)
1 Iowa corn field 10
2 Southern California strawberry field 10
3 Cardiff estuary sediment 10
4 Carlsbad lagoon sediment 10
5 Home compost 5
6 San Diego city compost 5
7 Commercial potting mixture 2.5
8 Commercial peat moss 2.5
1
Figure 2.
One microliter of total genomic DNA isolated using the DNeasy PowerMax Soil Kit was PCR amplified using primers to the Bacillus spp. Amplified DNA was analyzed on 0.8% TAE agarose and ethidium stained. The amount of PCR fragment produced in each sample is representative of the relative amount of the template sequence in the purified DNA. Different soil sources will have different types and amount of organisms providing genomic DNA. N = Negative control lacking template. P = Positive control template. Soil types and amount are identified in the table below.

Sample Lane Type Amount Processed (g)
1 Iowa corn field 10
2 Southern California strawberry field 10
3 Cardiff estuary sediment 10
4 Carlsbad lagoon sediment 10
5 Home compost 5
6 San Diego city compost 5
7 Commercial potting mixture 2.5
8 Commercial peat moss 2.5
2
Figure 3.
One microliter of total genomic DNA isolated using the DNeasy PowerMax Soil Kit was PCR amplified using primers to the Streptococcus spp. DNA was analyzed on 1 % TAE agarose and ethidium stained. The amount of PCR fragment produced in each sample is representative of the relative amount of template sequence in the purified. Different soil sources will have different types and amount of organisms providing genomic DNA. N = Negative control lacking template. P = Positive control template. Soil types and amount are identified below.

Sample Lane Type Amount Processed (g)
1 Iowa corn field 10
2 Southern California strawberry field 10
3 Cardiff estuary sediment 10
4 Carlsbad lagoon sediment 10
5 Home compost 5
6 San Diego city compost 5
7 Commercial potting mixture 2.5
8 Commercial peat moss 2.5
Performance



Features
Specifications
Bead size 0.7 mm garnet
Binding capacity Up to 1 mg per filter
Format Silica Spin Filter Tubes
Processing Bead beating
Sample size Up to 10 g
Sample types Processed soil, stool, gut material, biosolids
Storage temperature Store at room temperature(15-30°C)
Throughput 2 samples
Time per run or per prep 30 minutes
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