QIAamp RNA Blood Mini Kit

For purification of cellular RNA from fresh whole blood
  • Rapid purification of high-quality, ready-to-use RNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Removal of contaminants and inhibitors

The QIAamp RNA Blood Mini Kit provides silica-membrane-based purification of cellular RNA from up to 1.5 ml of fresh, whole human blood stabilized with any common anticoagulant, such as citrate, heparin, or EDTA. After homogenization using the QIAshredder spin column, a fast spin-column procedure simplifies RNA purification. Purification can be fully automated on the QIAcube Connect.

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产品 货号 目录价:
QIAamp RNA Blood Mini Kit (50)
For 50 RNA preps: 50 QIAamp Mini Spin Columns, 50 QIAshredder Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free reagents and buffers
52304
询价

QIAamp RNA Blood Mini Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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QIAamp RNA Blood Mini实验方案。|高品质RNA用于Northern分析。|可靠的RT-PCR分析。|
|使用QIAamp RNA Blood Mini Kit从0.5、1.0和1.5 ml(从左至右)健康的人全血(含有指定抗凝剂)中分离的总RNA的甲醛琼脂糖凝胶电泳及相应的Nothern印迹(GAPDH探针)。每个泳道上样60 µl洗脱液中的40微升。M:0.24–9.5 kb RNA分子量标准。|血液中的总RNA的RT-PCR。使用QIAamp RNA Blood Mini Kit从50 µl健康的人全血(含有指定抗凝剂)中纯化总RNA,使用50 µl不含RNA酶的水洗脱。对于每个样本,使用5 µl或15 µl洗脱液或对照(15 µl洗脱液,未进行RT)进行三次RT-PCR反应(从左至右)。进行RT-PCR时,使用不含RNA酶的DNA酶酶切样本并使用oligo-dT引物进行逆转录。使用GAPDH引物扩增1/10(2.5 µl)的cDNA。每个泳道上样1/5的PCR反应产物。:按上述步骤进行PCR,但以蒸馏水代替洗脱液;+:阳性对照cDNA片段。M:100 bp DNA分子量标准。|
性能

The QIAamp procedure completely removes RNases, contaminants, and enzyme inhibitors, yielding high-quality RNA suitable for any downstream application (see figures "High-quality RNA for northern analysis" and "Reliable RT-PCR analysis").

The QIAamp RNA Blood Mini Kit provides the highest-quality RNA with minimum copurification of DNA. However, as with any RNA purification method, some DNA contamination can be expected. For certain RNA applications that are sensitive to very small amounts of DNA, it may be necessary to remove any remaining DNA. In these cases, the QIAGEN RNase-Free DNase Set provides convenient on-column DNase treatment of RNA samples during QIAamp RNA procedures.

原理

The QIAamp RNA Blood Mini Kit provides purification of cellular RNA using silica-membrane technology. No phenol–chloroform extraction is required. RNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure RNA to be eluted in either water or a buffer provided with the kit.

QIAamp technology yields total cellular RNA from fresh whole blood and other sample sources that is ready to use in RT-PCR and blotting procedures. QIAamp sample preparation technology is fully licensed.

操作流程
The QIAamp RNA Blood Mini Kit simplifies isolation of RNA from blood with a fast spin-column procedure (see figure "Procedure"). Red blood cells are selectively lysed and white cells collected by centrifugation. White cells are then lysed using highly denaturing conditions which immediately inactivate RNases. After homogenization using the QIAshredder spin column, the sample is applied to the QIAamp spin column. Total RNA binds to the QIAamp membrane and contaminants are washed away, leaving pure RNA to be eluted in 30–100 µl RNase-free water (provided with the kit) for direct use in any downstream application.
应用

The QIAamp RNA Blood Mini Kit is designed for isolation of cellular RNA from up to 1.5 ml of fresh, whole human blood stabilized with any common anticoagulant, such as citrate, heparin, or EDTA. In addition, total cellular RNA can be isolated from tissue samples.

特点
参数
应用 PCR, real-time PCR, microarray
洗脱体积 30–100 µl
规格 Spin columns
主要样本类型 Whole blood, tissue
处理 Manual (centrifugation)
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 Cellular RNA
样本量 50–1.5 ml
技术 Silica technology
每次运行或制备时间 <1 hour
产量 1–5 µg

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For total RNA purification from human whole blood
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QIAamp RNA Blood Mini Procedure
QIAamp RNA Blood Mini实验方案。
High-quality RNA for northern analysis
高品质RNA用于Northern分析。
使用QIAamp RNA Blood Mini Kit从0.5、1.0和1.5 ml(从左至右)健康的人全血(含有指定抗凝剂)中分离的总RNA的甲醛琼脂糖凝胶电泳及相应的Nothern印迹(GAPDH探针)。每个泳道上样60 µl洗脱液中的40微升。M:0.24–9.5 kb RNA分子量标准。
Reliable RT-PCR Analysis  RT-PCR of total RNA from blood
可靠的RT-PCR分析。
血液中的总RNA的RT-PCR。使用QIAamp RNA Blood Mini Kit从50 µl健康的人全血(含有指定抗凝剂)中纯化总RNA,使用50 µl不含RNA酶的水洗脱。对于每个样本,使用5 µl或15 µl洗脱液或对照(15 µl洗脱液,未进行RT)进行三次RT-PCR反应(从左至右)。进行RT-PCR时,使用不含RNA酶的DNA酶酶切样本并使用oligo-dT引物进行逆转录。使用GAPDH引物扩增1/10(2.5 µl)的cDNA。每个泳道上样1/5的PCR反应产物。:按上述步骤进行PCR,但以蒸馏水代替洗脱液;+:阳性对照cDNA片段。M:100 bp DNA分子量标准。