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WeLLevator Pipette Guide

For the consistent and fatigue-free extraction of liquids from 96 well plates when using a multichannel pipette and enables the manual use of PowerMag and UltraMag kits when paired with a Magnetic Separator
  • Manually extract the maximum volume of supernatant from 96 well plates without concern about disturbing a pellet, and ensure uniform handling of samples in every well
  • Rest your multichannel pipette on top of the WeLLevator Pipette Guide for effortless pipetting
  • Increase your throughput without a robot: Convert our PowerMag and UltraMag magnetic bead kits to manual kits with just a WeLLevator and a Magnetic Separator

Manual extraction of liquids from 96 well plates using multichannel pipettes can be mechanically awkward and tedious. The sheer number of wells and the opacity of the plate make it difficult to avoid pelleted materials and to achieve well-to-well consistency. The patent-pending WeLLevator Pipette Guide (11970) is designed to facilitate consistent and fatigue-free extraction of liquids from SBS formatted 96 well plates when using a multichannel pipette. The WeLLevator Pipette Guide can be used with either 8 or 12 channel pipettes. 

Cat No./ID: 11970
WeLLevator Pipette Guide
445,00 €
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For the extraction of liquids from 96 well plates using a multichannel pipette and the manual use of MagAttract kits when paired with a Magnetic Separator
The WeLLevator Pipette Guide is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Figure 1a
The PowerMag DNA Clean-Up Kit effectively removes UV-absorbing contaminants from isolated DNA using either a KingFisher Duo or a WeLLevator Pipette Guide. (A) DNA isolated from 0.25 grams of garden soil without any upfront inhibitor removal (i.e., Inhibitor Removal Technology, IRT) is highly contaminated with UV-absorbing components that mask the true concentration of DNA found in the sample (“Garden soil, no IRT”). 100 µL technical replicates of this contaminated material was processed through the PowerMag DNA Clean-Up Kit on a KingFisher Duo or the MO BIO WeLLevator Pipette Guide equipped with a PowerMag Magnetic Separator to obtain highly purified, PCR-inhibitor depleted DNA (“PowerMag DNA Clean-Up”).

Note: Due to evaporative loss of water from the elution buffer during the KingFisher protocol, samples processed on the robot are usually more concentrated than those processed by hand on the WeLLevator. This is reflected here in the NanoDrop 260 nm absorbance reading.(B) Taking the final elution volumes into account, calculation of the % DNA recovery reveals that the WeLLevator-assisted magnetic bead protocol delivers excellent DNA yields and quality via spectrophotometric analysis. (C) DNA samples described in (B) were examined via 1.2% agarose gel, demonstrating that equivalent yields and high quality DNA are obtained using either a WeLLevator or KingFisher Duo protocol. 
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Figure 1b
The PowerMag DNA Clean-Up Kit effectively removes UV-absorbing contaminants from isolated DNA using either a KingFisher Duo or a WeLLevator Pipette Guide. (A) DNA isolated from 0.25 grams of garden soil without any upfront inhibitor removal (i.e., Inhibitor Removal Technology, IRT) is highly contaminated with UV-absorbing components that mask the true concentration of DNA found in the sample (“Garden soil, no IRT”). 100 µL technical replicates of this contaminated material was processed through the PowerMag DNA Clean-Up Kit on a KingFisher Duo or the MO BIO WeLLevator Pipette Guide equipped with a PowerMag Magnetic Separator to obtain highly purified, PCR-inhibitor depleted DNA (“PowerMag DNA Clean-Up”).

Note: Due to evaporative loss of water from the elution buffer during the KingFisher protocol, samples processed on the robot are usually more concentrated than those processed by hand on the WeLLevator. This is reflected here in the NanoDrop 260 nm absorbance reading.(B) Taking the final elution volumes into account, calculation of the % DNA recovery reveals that the WeLLevator-assisted magnetic bead protocol delivers excellent DNA yields and quality via spectrophotometric analysis. (C) DNA samples described in (B) were examined via 1.2% agarose gel, demonstrating that equivalent yields and high quality DNA are obtained using either a WeLLevator or KingFisher Duo protocol. 
2
Figure 1c
The PowerMag DNA Clean-Up Kit effectively removes UV-absorbing contaminants from isolated DNA using either a KingFisher Duo or a WeLLevator Pipette Guide. (A) DNA isolated from 0.25 grams of garden soil without any upfront inhibitor removal (i.e., Inhibitor Removal Technology, IRT) is highly contaminated with UV-absorbing components that mask the true concentration of DNA found in the sample (“Garden soil, no IRT”). 100 µL technical replicates of this contaminated material was processed through the PowerMag DNA Clean-Up Kit on a KingFisher Duo or the MO BIO WeLLevator Pipette Guide equipped with a PowerMag Magnetic Separator to obtain highly purified, PCR-inhibitor depleted DNA (“PowerMag DNA Clean-Up”).

Note: Due to evaporative loss of water from the elution buffer during the KingFisher protocol, samples processed on the robot are usually more concentrated than those processed by hand on the WeLLevator. This is reflected here in the NanoDrop 260 nm absorbance reading.(B) Taking the final elution volumes into account, calculation of the % DNA recovery reveals that the WeLLevator-assisted magnetic bead protocol delivers excellent DNA yields and quality via spectrophotometric analysis. (C) DNA samples described in (B) were examined via 1.2% agarose gel, demonstrating that equivalent yields and high quality DNA are obtained using either a WeLLevator or KingFisher Duo protocol. 
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