cador BVDV RT-PCR Kit
For sensitive and specific detection of BVDV in samples from cattle
The cador BVDV RT-PCR Kit is a highly sensitive and specific assay to screen for bovine viral diarrhea virus in samples from cattle. It provides detection of as few as 1.35 and 2.52 copies/μl of BVDV Type 1 and Type 2, respectively. The kit was co-developed with and validated by the Institute of Veterinary Virology, University of Bern, Switzerland, and validated by the Veterinary Laboratory Agency (VLA), Weybridge, UK. The kit is approved by the Friedrich-Löffler-Institut for veterinary diagnostic use in Germany. It is also recommended by the Swiss Federal Veterinary Office (Bundesamt für Veterinärwesen) and licensed for use in the BVDV eradication program in Switzerland.
The cador BVDV RT-PCR Kit is highly sensitive (see the figure "Sensitive detection of BVDV RNA"). The highly specific primer-probe sets and optimized amplification conditions of the protocols ensure 100% specific detection of BVDV.
The kit performs well across a whole range of animal sample types (see the figure "Detection of BVDV RNA from a variety of sample types"). BVDV RNA was isolated from bovine whole blood, plasma, and serum samples and reliably detected even at dilutions of 1:10,000. The kit also performs well with viral RNA isolated from milk and ear notch samples.
PCR-based methods of pathogen detection provide advantages over traditional methods such as culture and ELISA tests. Culture requires extended time and viable virus. Antigen ELISA tests can provide false negative results with infected calves because the high levels of maternal antibodies from the colostrum can mask the virus. The cador BVDV RT-PCR Kit eliminates these challenges by using real-time PCR technology to detect the viral RNA.
Polymerase chain reaction (PCR) is based on the amplification of specific regions of the pathogen genome. In real-time PCR and RT-PCR, the amplified product is detected using fluorescent dyes. These are usually linked to oligonucleotide probes that bind specifically to the amplified product. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating product without the need to re-open the reaction tubes afterward.
The cador BVDV RT-PCR Kit contains primers and probes specific for a highly conserved region of the BVDV genome, plus an internal control to identify possible PCR inhibition, and 2 external positive controls. It can reliably detect BVDV Type 1 and BVDV Type 2 RNA.
For specific detection and differentiation of BVDV Type 1, BVDV Type 2, and border disease virus, see the cador BVDV Type 1/2 RT-PCR Kit.
Viral RNA can be manually isolated from a whole range of animal samples, including undiluted whole blood, using the dedicated QIAamp cador Pathogen Mini Kit. Purification of viral RNA using this kit is fully automatable on the QIAcube. The real-time RT-PCR detection of the viral RNA using the cador BVDV RT-PCR Kit is then performed on a real-time PCR instrument, such as the Rotor-Gene Q.
Viral RNA can also be isolated from ear notch samples using the RNeasy Fibrous Tissue Kit, and from milk pellets using the RNeasy Mini or RNeasy 96 Kit with the QIAshredder homogenizer. These isolation procedures are also fully automatable.
The cador BVDV RT-PCR Kit is designed to screen for bovine viral diarrhea virus infections in samples from cattle.
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