RNeasy Plant Mini Kit

For purification of total RNA from plants and fungi
  • High-quality total RNA in 30 minutes
  • No phenol/chloroform extraction
  • No CsCl gradients, no LiCl or ethanol precipitation
  • Excellent recovery of RNA
  • Ready-to-use RNA for any downstream application

The RNeasy Plant Mini Kit includes QIAshredder spin columns for homogenizing and filtering viscous plant or fungal lysates, and RNeasy spin columns for purifying up to 100 μg of high-quality RNA using silica-membrane technology. Purification can be fully automated on the QIAcube Connect. The kit can also be used in combination with a TissueRuptor or TissueLyser system, which provide efficient disruption and homogenization of plant samples.

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产品 货号 目录价:
RNeasy Plant Mini Kit (50)
50 RNeasy Mini Spin Columns, 50 QIAshredder Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
74904
询价

RNeasy Plant Mini Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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RNeasy Plant Mini实验流程。|病毒检测。|组蛋白H4的组织特异性表达。|
|采用改进的RNeasy实验方案从被感染的苹果(1)、樱桃(2)、梨(3)和葡萄藤(4–8)的种苗、黑加仑(9, 10)的叶组织及被感染的马铃薯的休眠块茎组织(11, 12)中分离总RNA。利用单管RT-PCR扩增病毒RNA。在1.5%的琼脂糖凝胶上分析扩增产物。M:DNA Markers VI,Boehringer Mannheim。(数据由加拿大农业及农业食品部植物健康中心的D.J. MacKenzie以及加拿大悉尼Euro Nursery & Vineyard(West)Inc.的M.A. McLean提供。)|图示的番茄组织的总RNA(7 µg)的甲醛琼脂糖凝胶和northern印迹。采用32P标记的针对番茄组蛋白H4基因的DNA探针杂交印迹。M:0.24-9.5 kb RNA分子量标准。(植物组织和组蛋白H4 cDNA克隆由德国科隆马克斯-普朗克研究所从事育种研究的K. Theres提供。)|
性能

The RNeasy Plant Mini Kit is ideal for isolation of total RNA from a wide variety of plant and fungal samples with sample sizes of 10–100 mg tissue, or 100–1 x 107 cells (see table "Selected samples processed with the RNeasy Plant Mini Kit"). The binding capacity of the spin-columns is up to 100 µg RNA. The typical yield from 100 mg plant tissue is between 25 µg and 65 µg of RNA, although amounts of RNA can vary due to the developmental stage and growth conditions of the samples (see table "Yield from 100 mg tissue").

Selected samples processed with the RNeasy Plant Mini Kit
PlantsFilamentous fungi
Anemone sp.
Arabidopsis thaliana1
Begonia sp.
Beta vulgaris (sugar beet)2
Chlamydomonas reinhardii (unicellular alga)
Chrysanthemum
Clarkia spp.3
Daucus carota (carrot)4
Diascia sp.
Dendranthema sp.
Euglena gracilis (unicellular alga)
Funaria hygrometrica (moss)
Hordeum vulgare (barley)
Lycopersicon esculentum (tomato)5
Malus sp. (apple)6
Nicotiana tabacum (tobacco)
Oryza sativa (rice)
Pelargonium sp. (geranium)
Petroselinum crispum (parsley)7
Pisum sativum (pea)
Prunus sp. (cherry)6
Ranunculus sp.
Ribes nigrum (black currant)
Riccia fluitans (liverwort)
Sinapis arvensis (mustard)
Solanum tuberosum (potato)8
Spinacia oleracea (spinach)
Surfinia sp.
Triticum aestivum (wheat)
Vitis sp. (grapevine)6
Zea mays (maize)
Acremonium crysogenum9
Fusarium avenaceum9
Young leaves or needles, except as indicated: 1 Flowers, buds, siliques, roots; 2 roots; 3 petals, stamen, stigma, sepals, style; 4 cultured cells; 5 mature flower, expanded leaves, shoot tip, stem, green fruit; 6 budwood; 7 seedlings; 8 dormant tuber; 9 mycelium

 

Yield from 100 mg tissue
Sample typeYield
Arabidopsis leaves 35 µg
Maize leaves 25 µg
Tomato leaves 65 µg
Tobacco leaves 60 µg

The purified plant RNA, eluted in 30–100 µl volume, includes viral RNA (see figure "Detection of viruses"). All contaminants, such as polysaccharides, are removed, and the eluted RNA is ready for all downstream applications (see figure "Tissue specificity of histone H4 expression").

原理

The RNeasy Plant Mini Kit provides QIAshredder columns for homogenization and filtration of viscous plant or fungal lysates by microcentrifugation in combination with RNeasy Mini spin columns for RNA purification. RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification. RNeasy Kits provide the highest-quality RNA with minimum copurification of DNA. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using convenient on-column DNase treatment during the RNeasy procedure.

操作流程
Samples are first lysed and then homogenized in the QIAshredder columns. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the RNeasy silica membrane. RNA binds, and all contaminants are efficiently washed away. Pure, concentrated RNA is eluted in water (see flowchart "RNeasy Plant Mini procedure").
应用

RNA purified with RNeasy technology is high-quality and is ideal for use in all applications. Downstream applications include:

Northern, dot, and slot blotting
End-point RT-PCR
Quantitative, real-time RT-PCR
Array analysis
Poly A+ RNA selection
特点
参数
应用 PCR, qPCR, real-time RT-PCR, microarray
洗脱体积 30–100 µl
规格 Spin column
主要样本类型 Plant samples
处理 Manual
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 RNA
样本量 10–100 mg
技术 Silica technology
每次运行或制备时间 30 minutes
产量 25–60 µg

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试剂盒操作手册
1
RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungi
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参考文献
0
图片
RNeasy Plant Mini Procedure
RNeasy Plant Mini实验流程。
Detection of viruses from a variety of plant species by RT-PCR
病毒检测。
采用改进的RNeasy实验方案从被感染的苹果(1)、樱桃(2)、梨(3)和葡萄藤(4–8)的种苗、黑加仑(9, 10)的叶组织及被感染的马铃薯的休眠块茎组织(11, 12)中分离总RNA。利用单管RT-PCR扩增病毒RNA。在1.5%的琼脂糖凝胶上分析扩增产物。M:DNA Markers VI,Boehringer Mannheim。(数据由加拿大农业及农业食品部植物健康中心的D.J. MacKenzie以及加拿大悉尼Euro Nursery & Vineyard(West)Inc.的M.A. McLean提供。)
Tissue specificity of histones H4 expression.
组蛋白H4的组织特异性表达。
图示的番茄组织的总RNA(7 µg)的甲醛琼脂糖凝胶和northern印迹。采用32P标记的针对番茄组蛋白H4基因的DNA探针杂交印迹。M:0.24-9.5 kb RNA分子量标准。(植物组织和组蛋白H4 cDNA克隆由德国科隆马克斯-普朗克研究所从事育种研究的K. Theres提供。)