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This protocol provides information about scaling of reagents required for purification of DNA from 5-60 mg mouse tail tissue using the Gentra Puregene Mouse Tail Kit.
This protocol provides information about scaling of reagents required for purification of DNA from 100 to 5 x 108 cultured cells using the Gentra Puregene Cell Kit.
This protocol provides information about scaling of reagents required for purification of DNA from 1 x 106 - 3 x 107 fixed cells using the Gentra Puregene Tissue Kit.
This protocol provides information about scaling of reagents required for purification of DNA from 0.5-700 mg solid tissue using the Gentra Puregene Tissue Kit.
This protocol provides information about scaling of reagents required for purification of DNA from 0.5-20 mg paraffin-embedded tissue using the Gentra Puregene Tissue Kit.
Endotoxin-free DNA is essential for gene therapy research and will improve transfection into sensitive eukaryotic cells.
This protocol is designed for rapid purification of DNA from 1-2 x 106, 3-5 x 106, or 6-9 x 106 cells using the Gentra Puregene Cell Kit.
Up to 1 x 109 bacteria are disrupted and homogenized by bead-milling in a guanidine-thiocyanate-containing lysis buffer. After addition of ethanol, the sample is loaded onto an RNeasy Mini spin column. Total RNA binds to the RNeasy silica-membrane, contaminants are efficiently washed away, and high-quality RNA is eluted in RNase-free water.
This protocol is designed for purification of DNA from up to 5 x 107 yeast cells.
This protocol is designed for purification of DNA from up to 50 mg of insects, such as drosophila.
This protocol is designed for purification of DNA from a 200 μl crude lysate.
There are two protocols: follow Protocol 1 if you want to purify total RNA containing miRNA, or follow Protocol 2 if you want to purify small RNA (includes miRNA, 5S rRNA, and tRNA) and larger RNA (>200 nt) separately.
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