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Whole genome amplification from biopsies using the REPLI-g Advanced DNA Single Cell Kit
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Supplementary Protocols
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Whole genome amplification from buccal cells using the REPLI-g Advanced DNA Single Cell Kit
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Supplementary Protocols
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Whole genome amplification from flash-frozen tissue sections using the REPLI-g Advanced DNA Single Cell Kit
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Supplementary Protocols
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Whole genome amplification from single cells using the REPLI-g Advanced DNA Single Cell Kit
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Supplementary Protocols
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Purification of REPLI-g amplified DNA by LiCl/EtOH precipitation
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Supplementary Protocols
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Purification of REPLI-g amplified DNA using Agencourt® AMPure® XP magnetic beads
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Supplementary Protocols
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Enrichment of Poly(A)+ RNA in 200 μl plates Supplementary Supplement
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Supplementary Protocols
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QIAseq miRNA Primary Quantification Supplementary Protocol
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Supplementary Protocols
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GeneRead Size Selection Q QIAcube Protocol
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Kit Handbooks
Supplementary Protocols
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Supplementary Protocol: Isolation of total RNA, including small RNAs, from plant tissues
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Supplementary Protocols
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QIAseq FastSelect −rRNA HMR and/or −Globin with the Truseq Stranded Total RNA Library Prep
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Supplementary Protocols
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QIAseq miRNA Library Ion Chef and Ion S5 setup
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Supplementary Protocols
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GeneRead DNA Library Q QIAcube Protocol Sheet V2
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Kit Handbooks
Supplementary Protocols
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Microbial DNA qPCR Array ABI7900 Instrument Setup
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Supplementary Protocols
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Purification of MAP DNA from feces using the QIAamp® DNA Stool Mini Kit
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Supplementary Protocols
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Microbial DNA qPCR Array Rotor-Gene Q Instrument Setup
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Supplementary Protocols
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ABI 7500 & ABI 7500 FAST (Software Version 2.0.4) instrument setup instructions for RT2 Profiler PCR Arrays
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Supplementary Protocols
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ABI 7900HT (for SDS Software 2.1, 2.3 and 2.4) instrument setup instructions for RT2 Profiler PCR Arrays
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Supplementary Protocols
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ABI StepOnePlus (for Software Version 2.0) instrument setup instructions for RT2 Profiler PCR Arrays
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Supplementary Protocols
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Bio-Rad CFX96 and CFX384 instrument setup instructions for RT2 Profiler PCR Arrays
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Supplementary Protocols
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Bio-Rad iCycler & iQ Real-Time PCR Systems (for Software Version 3.1) instrument setup instructions for RT2 Profiler PCR Arrays
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Supplementary Protocols
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Eppendorf Mastercycler ep realplex instrument setup instructions for RT2 Profiler PCR Arrays
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Supplementary Protocols
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Stratagene Mx3000P qPCR System real-time PCR run setup instructions for RT2 Profiler PCR Arrays
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Supplementary Protocols
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Rotor-Gene Q real-time PCR run setup instructions for RT2 Profiler PCR Arrays
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Supplementary Protocols
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Roche LightCycler 480 real-time PCR run setup instructions for RT2 Profiler PCR Arrays
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Supplementary Protocols
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Life Technologies ViiA7 (ViiA 7 Software v1.2) instrument setup instructions for RT2 Profiler PCR Arrays
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Supplementary Protocols
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Microbiome stabilization using the RNAprotect Tissue Reagent
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Supplementary Protocols
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Supplementary Protocols: Single Cell WGA — Purification of Amplified DNA
Purification of DNA after whole Genome amplification using the REPLI-g Single Cell Kit
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Supplementary Protocols
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Purification of total RNA from tissues and cells using the BioSprint® 96 One-For-All Vet Kit with additional DNase I treatment - (EN)
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Supplementary Protocols
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Purification of total DNA from ticks using the DNeasy® Blood & Tissue Kit for detection of Borrelia DNA - (EN)
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Supplementary Protocols
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Sequencing Library Preparation from Cell-Free DNA for Illumina® Sequencing Platforms
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Supplementary Protocols
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Sequencing Library Preparation Using Agencourt® AMPure® XP Beads to Remove Adapter Dimers - (EN)
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Supplementary Protocols
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Test Assay for Fast Reaction Mix 2.0 - (EN)
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Supplementary Protocols
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Polyacrylamide gel analysis of oligonucleotides
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Supplementary Protocols
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Applied Biosystems 7500 and 7500 FAST (software version 2.0.4) instrument setup instructions for EpiTect ChIP PCR Arrays
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Supplementary Protocols
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Applied Biosystems 7900 HT (for SDS Software 2.1, 2.3, and 2.4) instrument setup instructions for EpiTect ChIP PCR Arrays
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Supplementary Protocols
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Applied Biosystems StepOnePlus (for software version 2.0) instrument setup instructions for EpiTect ChIP PCR Arrays
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Supplementary Protocols
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Eppendorf Mastercycler ep realplex instrument setup instructions for EpiTect ChIP PCR Arrays
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Supplementary Protocols
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Stratagene Mx3000P qPCR System instrument setup instructions for EpiTect ChIP PCR Arrays
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Supplementary Protocols
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Life Technologies ViiA7 (for ViiA7 Software version 1.2) instrument setup instructions for EpiTect ChIP PCR Arrays
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Supplementary Protocols
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Applied Biosystems 7900 HT (for SDS Software 2.1, 2.3, and 2.4) instrument setup instructions for EpiTect Methyl II PCR Arrays
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Supplementary Protocols
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Microbial DNA qPCR Array ABI7500 Instrument Setup
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Supplementary Protocols
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Microbial DNA qPCR Array ViiA7 Instrument Setup
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Supplementary Protocols
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Roche LightCycler 480 real-time PCR setup instructions for miScript miRNA PCR Arrays
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Supplementary Protocols
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Applied Biosystems 7500 and 7500 FAST (software version 2.0.4) instrument setup instructions for qBiomarker Copy Number PCR Arrays
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Supplementary Protocols
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Applied Biosystems 7900 HT (for SDS Software 2.1, 2.3, and 2.4) instrument setup instructions for qBiomarker Copy Number PCR Arrays
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Supplementary Protocols
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Applied Biosystems StepOnePlus (for software version 2.0) instrument setup instructions for qBiomarker Copy Number PCR Arrays
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Supplementary Protocols
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Life Technologies ViiA7 (for ViiA7 Software version 1.2) instrument setup instructions for qBiomarker Copy Number PCR Arrays
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Supplementary Protocols
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Bio-Rad CFX96 and CFX384 instrument setup instructions for qBiomarker Copy Number PCR Arrays
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Supplementary Protocols
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Bio-Rad iCycler and iQ Real-Time PCR Systems instrument setup instructions for qBiomarker Copy Number PCR Arrays
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Supplementary Protocols
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Eppendorf Mastercycler ep realplex instrument setup instructions for qBiomarker Copy Number PCR Arrays
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Supplementary Protocols
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Stratagene Mx3000P qPCR System instrument setup instructions for qBiomarker Copy Number PCR Arrays
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Supplementary Protocols
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Roche LightCycler 480 instrument setup instructions for qBiomarker Copy Number PCR Arrays
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Supplementary Protocols
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Roche LightCycler 480 real-time PCR run setup instructions for qBiomarker Somatic Mutation PCR Arrays
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Supplementary Protocols
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Applied Biosystems ViiA7 real-time PCR run setup instructions for qBiomarker Somatic Mutation PCR Arrays
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Supplementary Protocols
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Deparaffinization of PAXgene® Tissue fixed, paraffin-embedded tissue sections with Deparaffinization Solution - (EN)
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Supplementary Protocols
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Cryo-embedding tissue specimens fixed and stabilized with the PAXgene® Tissue System - (EN)
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Supplementary Protocols
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Preparation of PFPE tissue sections for use with in situ hybridization (ISH) staining assays - (EN)
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Supplementary Protocols
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Purification of archive-quality DNA from 50 µl CSF using the Gentra® Puregene® Tissue Kit - (EN)
This protocol is designed for purification of DNA from 50 μl samples of CSF using the Gentra Puregene Tissue Kit.
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Supplementary Protocols
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NGS Library Preparation for Whole Genome Bisulfite Sequencing (WGBS) on Illumina® Sequencing Platforms - (EN)
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Supplementary Protocols
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