Part 1: RT primer hybridization, reverse transcription, second strand synthesis, end-repair, A-addition
Part 2: Adapter ligation, cleanup of adapter-ligated DNA, target enrichment
Part 3: Cleanup of target enrichment, universal PCR, cleanup of universal PCR
Part 3: Adapter ligation and universal PCR
Part 1: Cell lysis and reverse transcription
Part 2: Template amplification and fragmentation, end-repair, and A-addition
Part 1: Cell lysis, reverse transcription
Part 2: Single primer extension
Part 3b: Library amplification using tube indices
Part 1: 3' Ligation, 5' ligation, reverse transcription
Part 2: QIAseq miRNA NGS (QMN) Bead preparation, cDNA cleanup
Part 1: Isolation of mRNA and reverse transcription
Part 2: Preamplification PCR and qRT-PCR
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
For use with the RNeasy 96 QIAcube HT Kit and QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
Adaptions for using the comparative CT method (2–ΔΔCq)
For fast, efficient, and optimization-free multiplex PCR for advanced applications
Part 3a: Library amplification using HT plate indices
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