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The following protocol is optimized for transient transfection of HeLa-S3 cells in 96-well plates. Please note that for HeLa cells the optimal amounts of DNA and PolyFect® Reagent differ from the amounts given in the protocol below. Please call one of the QIAGEN Technical Service Departments or your local distributor for an optimized HeLa protocol.
The following protocol is optimized for transient transfection of HeLa-S3 cells in 60 mm dishes. Parameters for transfection using other culture formats are given in Table 1. Please note that this protocol is only for HeLa-S3 cells. Optimized protocols for HeLa cells are provided in the PolyFect® Transfection Reagent Handbook.
The following protocol is optimized for transient transfection of HeLa cells in 96-well plates. Please note that for HeLa-S3 cells the optimal amounts of DNA and PolyFect® Reagent differ from the amounts given in the protocol below. Please call one of the QIAGEN Technical Service Departments or your local distributor for an optimized HeLa-S3 protocol.
The following protocol is optimized for transient transfection of COS-7 cells in 96-well plates.
The following protocol is optimized for transient transfection of CHO cells in 96-well plates.
The following protocol is optimized for transient transfection of 293 cells in 96-well plates.
Some RNAi applications require the use of low amounts of siRNA. This supplementary protocol is a modified version of the RNAiFect Transfection Reagent protocol (see RNAiFect Transfection Handbook), that is optimized for transfection of adherent cells with low siRNA concentrations.
Software assay package for use with the therascreen KRAS RGQ PCR Kit and Rotor-Gene Q 5plex HRM or Rotor-Gene Q MDx 5plex HRM in combination with Rotor-Gene Q Software version 2.3 and 72-well rotor.
Installation of the therascreen KRAS Assay Package is described in the therascreen KRAS RGQ PCR Kit Handbook.  
Note: Previous versions of the assay package, including the version listed in the current kit handbook, are not compatible with Rotor-Gene Q Software version 2.3. If your Rotor-Gene Q 5plex HRM or Rotor-Gene Q MDx 5plex HRM has an earlier software version installed, you can upgrade to Rotor-Gene Q Software version 2.3 by downloading the software from the Rotor-Gene Q or Rotor-Gene Q MDx product webpages.
Software assay package for use with the therascreen BRAF RGQ PCR Kit and Rotor-Gene Q 5plex HRM or Rotor-Gene Q MDx 5plex HRM in combination with Rotor-Gene Q Software version 2.3 and 72-well rotor.
Installation of the therascreen BRAF Assay Package is described in the therascreen BRAF RGQ PCR Kit Handbook.  
Note: If your Rotor-Gene Q 5plex HRM or Rotor-Gene Q MDx 5plex HRM has an earlier software version installed, you can upgrade to Rotor-Gene Q Software version 2.3 by downloading the software from the Rotor-Gene Q or Rotor-Gene Q MDx product webpages.
Purification of DNA after whole Genome amplification using the REPLI-g Single Cell Kit
This protocol provides information about scaling of reagents required for purification of DNA from 1-10 ml samples of fresh or frozen whole blood using the Gentra Puregene Blood Kit.
This protocol provides information about scaling of reagents required for purification of DNA from buffy coat prepared from 0.3-15 ml samples of whole blood using the Gentra Puregene Blood Kit.
This protocol provides information about scaling of reagents required for purification of DNA from 5-60 mg mouse tail tissue using the Gentra Puregene Mouse Tail Kit.
This protocol provides information about scaling of reagents required for purification of DNA from 100 to 5 x 108 cultured cells using the Gentra Puregene Cell Kit.
This protocol provides information about scaling of reagents required for purification of DNA from 1 x 106 - 3 x 107 fixed cells using the Gentra Puregene Tissue Kit.
This protocol provides information about scaling of reagents required for purification of DNA from 0.5-700 mg solid tissue using the Gentra Puregene Tissue Kit.
This protocol provides information about scaling of reagents required for purification of DNA from 0.5-20 mg paraffin-embedded tissue using the Gentra Puregene Tissue Kit.
Endotoxin-free DNA is essential for gene therapy research and will improve transfection into sensitive eukaryotic cells.
This protocol is designed for rapid purification of DNA from 1-2 x 106, 3-5 x 106, or 6-9 x 106 cells using the Gentra Puregene Cell Kit.
Up to 1 x 109 bacteria are disrupted and homogenized by bead-milling in a guanidine-thiocyanate-containing lysis buffer. After addition of ethanol, the sample is loaded onto an RNeasy Mini spin column. Total RNA binds to the RNeasy silica-membrane, contaminants are efficiently washed away, and high-quality RNA is eluted in RNase-free water.
This protocol is designed for purification of DNA from up to 5 x 107 yeast cells.
This protocol is designed for purification of DNA from up to 50 mg of insects, such as drosophila.
This protocol is designed for purification of DNA from a 200 μl crude lysate.
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