How is the artus® kit specificity determined?

The specificity of the detection system for a particular pathogen is ensured by the careful selection of highly specific primers and probes as well as by stringent reaction conditions. The sequence specificity of the primers and probes is verified by homology searches and sequence alignments with the pathogen's and closely related sequences.

The specificity is further controlled by assaying the kit system with isolates of closely related pathogens, pathogens causing similar symptoms, and, if applicable, with isolates of different genotypes/subtypes of the pathogen of interest.

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