miRNA discovery using NGS

Mar 21, 2018 1:00 PM–2:00 PM (EST)
Duration: 1hrs

miRNAs are naturally occurring, noncoding RNAs that mediate post-transcriptional gene regulation. miRNAs are small, around 22 nucleotides long, but mighty. Serving as regulators of gene expression with an important role in many biological processes, miRNAs are broadly studied. There are many tools and methods available to support miRNA research – choosing the right tools for your miRNA experiments is essential for success and bringing you closer to your next big discovery.

Part 1: Advancing miRNA research – best practices for your experiments
This webinar will guide you through the steps of miRNA experiments. We will explain best practices and tools to consider at each step, and the pros and cons of the different assay methods. We will also explain how to isolate miRNA from different sample types.

Part 2: Introduction to miRNA discovery using NGS
In this webinar, we will introduce the QIAseq miRNA Library Kit which features unique molecular indexing (UMIs) and a gel-free workflow from as little as 1 ng of total RNA.  Whether you’re working with serum, cells, tissues or FFPE samples, QIAGEN’s LNA-enhanced chemistry and proprietary technology ensures higher levels of mapped miRNA reads. Cloud-based NGS data analysis allows anyone with access to an NGS instrument to go from sample to data in as little as 3 days.  Come join us as we go through the best practices for miRNA-seq library construction and data analysis.

Part 3: Successfully detect miRNAs using qPCR with LNA technology

This webinar will introduce the advantages of miRNA detection using qPCR with LNA (locked nucleic acids) technology. In addition, we will discuss how to overcome the challenges of analyzing biofluids such as plasma.

Melanie Hussong

Dr. Melanie Hussong is a an experienced molecular biologist and geneticist  with 12+ years of experience in the area of Next-generation sequencing and its application in the clinical research environment.   Dr. Hussong received her Ph.D. from University of Melbourne, Australia and has published numerous papers and presented extensively in the field of NGS.  Prior to joining QIAGEN she pioneered the use of direct capture method for genome partitioning  resulting in the development of novel library construction  strategies.  Dr. Hussong is a recent addition to the QIAGEN family  and currently working on developing the  new products for RNA sequencing.