Reliable assessment of limit of detection (LOD) of viral particles
Reliable assessment of limit of detection (LOD) of viral particles
Six transport media compatible with the kit
Six transport media compatible with the kit
An innovative 3-step liquid-based workflow
An innovative 3-step liquid-based workflow
QIAprep& Viral RNA UM Kit performance comparison between saliva and gargle samples.
QIAprep& Viral RNA UM Kit performance comparison between saliva and gargle samples.
Protocol comparison of QIAprep& Viral RNA UM Kit performance with saliva samples
Protocol comparison of QIAprep& Viral RNA UM Kit performance with saliva samples
Reliable assessment of limit of detection (LOD) of viral particles Limit of Detection (LOD) tested on inactivated SARS-CoV-2 viral particles at 250, 500, 1000, 2000 and 4000 cp/ml. Type of sample diluted in UTM + cell line KG1A at different cp/reaction. All samples tested with KG1A (cell line) amount into the reaction: 1200 cells per reaction. LOD of 8 copies/reaction offers the test sensitivity required.
Six transport media compatible with the kit The kit is compatible with samples collected in non-fixation transport media like UTM, VTM, PBS, 0.9% NaCl, Virocult and eSwab. 8 µl of respective transport medium spiked with indicated amount of IVT + 2 µl Viral RNA UM Prep Buffer + 10 µl Viral RNA UM Master Mix show comparable Ct values.
An innovative 3-step liquid-based workflow

An aliquot is taken from a primary sample (nasopharyngeal, oropharyngeal or nasal swab) in transport media as starting material. This is added to an optimized sample preparation buffer, which allows preparation of the viral RNA template without degradation in two minutes, before being combined with an RT-qPCR reaction mix for rapid amplification on any thermocycler using any assay. The output is finally interpreted, delivering test results in under one hour.
QIAprep& Viral RNA UM Kit performance comparison between saliva and gargle samples. Positive high viral titer swab samples spiked into negative saliva and gargle samples. Samples heat-treated at 95°C for 15 min using the QIAprep& workflow for saliva or treated with QIAprep& Buffer AB and incubated at 95°C for 5 min.
Protocol comparison of QIAprep& Viral RNA UM Kit performance with saliva samples Saliva samples (n=20). Samples 1–17: Positive donors. Samples 18–20: Negative donors. Same samples differentially treated. Protocol 1:QIAprep& Viral RNA UM Kit without Buffer AB (95°C, 15 min). Protocol 2:QIAprep& Viral RNA UM Kit with Buffer AB.