Flexibility and accuracy with QIAxpert Slides
QIAxpert uses disposable microfluidic slides to analyze up to 16 samples per run. On-board capillary intake and storage channels ensure easy sample loading with no cross-contamination, and allow unused positions to be used in later analyses. The capillary channels preserve samples and prevent evaporation (see figure Microfluidic features of the QIAxpert Slide
). Thus, samples can be loaded and left at room temperature for up to 2 hours prior to readout. Constructed from advanced technical plastics that allow high optical transmission over the full UV/VIS spectrum, QIAxpert Slides enable highly accurate readings from just 2 µl of sample.
Smart analysis protocols based on cutting-edge bioinformatics
The QIAxpert performs classic nucleic acid and protein quantification based on absorbance measurements. In addition, smart analysis protocols called “apps” come pre-installed to quantify specific analytes of interest. Based on top bioinformatics expertise and extensive sample testing, the spectral profiling protocols let users focus on the molecule of interest, subtracting other absorbing impurities and background turbidity.
Unique spectral content profiling
To addresss challenging research samples, such as difficult-to-isolate or amplified nucleic acids, unique spectral protocols accurately quantify complex samples containing multiple UV/VIS absorbing chemicals that interfere with classical absorbance methods. Intelligent software algorithms extract from the measured UV/VIS spectrum the contributions of specific components like DNA, RNA, and impurities (see figure RNA spectral profiling analysis
). The quantity of these components is then calculated. These data are very helpful to analyses such as quantitative gene expression by qPCR, where quality of purified RNA is crucial because gDNA contamination leads to decreased accuracy of results.