Pyrosequencing: PyroMark Q24 & PyroMark Q24 Advanced

For reagents and support of discontinued instruments PyroMark Q24 and PyroMark Q24 Advanced

S_2018_ADNA_PyroM001_s

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PyroMark OneStep RT-PCR Kit (50)

Cat no. / ID.   978801

For 50 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water
€270.00
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KitAssayAccessoriesReagentSoftware
PyroMark Kits
Assays and Tests
Buffers
Q24 Reagents
Consumables
PyroMark Q24 Software
PyroMark Software
Consumables (IVD)
Type
OneStep RT-PCR Kit
PyroMark PCR Kit
Reactions
50
200
This product contains substances regulated under REACH (EC 1907/2006 Annex XIV). The use of this product in the EU is permitted subject to an exemption (Article 56(3)). Please refer to the REACH notification and the SDS of this product, both of which can be found in the “Resources” section of this page, for more information.
The PyroMark Q24, Q24 Advanced and Q48 Autoprep instruments and corresponding software are intended to be used only in combination with QIAGEN kits indicated for use with the instruments for the applications described in the kit handbooks. If the PyroMark instruments and software are used with other than QIAGEN kits, it is the user's responsibility to validate the performance of such product combination for any particular application.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Consider upgrading to a PyroMark Q48 Autoprep System for these advantages

  • Automation of the Pyrosequencing protocol without the need for a manual process on a vacuum prep workstation
  • Up to 48 samples can be analyzed
  • Compact instrument size
  • Enabled for connectivity
  • Touch screen display that guides users through the process

Product Details

The PyroMark Q24 was discontinued in 2018. The instrument is no longer sold, but existing instruments will be supported until the end of 2026. In addition, reagents will continue to be available until the end of 2026.

To future-proof your Pyrosequencing assays, consider upgrading your work to using a PyroMark Q48 Autoprep System which is actively sold and supported by QIAGEN.

Resources

Download Files (28)
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0011
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0012
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0007
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0015
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0014
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0008
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0008
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0013
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0004
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0002
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0004
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0009
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0011
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0005
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0010
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0005
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0013
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0003
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0012
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0001
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0006
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0010
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0006
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0009
Instrument methods file for use with PyroMark Q24 Advanced Software, PyroMark Q24 Advanced Reagents or PyroMark Q24 Advanced CpG Reagents, and PyroMark Q24 Cartridges marked with method 0003
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0007
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0014
Instrument methods file for use with PyroMark Q24 Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0015
Kit Handbooks (17)
September 2024
For performing Pyrosequencing reactions on the PyroMark Q24
For use with PyroMark Q48 Autoprep, PyroMark Q24 Advanced, PyroMark Q24, PyroMark Q96 ID and PyroMark Q96 MD systems
October 2009
September 2024
For quantification of methylation level of the MLH1 gene using PyroMark Q24, PyroMark Q24 Advanced, and PyroMark Q48 Autoprep
For quantification of methylation level of the MGMT gene using PyroMark Q24, PyroMark Q24 Advanced, and PyroMark Q48 Autoprep
Safety Data Sheets (1)
Operating Software (4)
This version is compatible with Windows 7, Windows 10, and Windows 11 (64 bit) operating systems. This software may only be downloaded by registered users with a valid PyroMark Q24 software license and registered PyroMark Q24 instrument. If you do not have a valid software license, contact your QIAGEN sales representative.
Files for upgrading existing PyroMark Q24 instruments to firmware 1.5.6903
PyroMark Q24 Advanced Software version 3.0.1 is compatible with Windows 7, Windows 10, and Windows 11 (64 bit) operating systems. This software may only be downloaded by registered users with a valid PyroMark Q24 Advanced Software license and registered PyroMark Q24 Advanced instrument. If you do not have a valid software license, contact your QIAGEN sales representative.
PyroMark Assay Design Software version 2.0.2 is compatible with Windows 7, Windows 10, and Windows 11 (64 bit) operating systems. This software may only be downloaded by registered users with a valid PyroMark Assay Design Software license. If you do not have a valid software license, contact your QIAGEN sales representative.
Software User Guides (1)
Brochures & Guides (1)
Certificates of Analysis (1)
Technical Information and Important Notes (1)

FAQ

How much space does the PyroMark Q24 instrument need?

PyroMark Q24 takes very little bench space, measuring only H420 x W390  x D525 mm.

 

FAQ-2098
Can I order the nucleotides from PyroMark Gold Reagents separately?
The nucleotides can only be ordered as part of the PyroMark Gold Reagents which also contain enzyme and substrate mix.
FAQ-2827
Can PyroMark Gold reagents be vortexed?
Reconstiuted enzyme and substrate of PyroMark Gold Reagents, should not be vortexed since this could lead to conformational changes which affect the activity.
FAQ-2844
Does the PyroMark CpG LINE assay target mouse transposons as well?

The PyroMark CpG LINE-1 assay is specific for human DNA and was not tested on mouse DNA.  Mouse LINE-1 elements differ slightly in sequence and may not be detected.

 

 

FAQ-2859
Which operating system is compatible with PyroMark Q24 Advanced application software?

The new PyroMark Q24 Advanced Software (version 3.0.1) is compatible with Windows XP, Vista (32 and 64 bit), Windows 7 (32 and 64 bit), and Windows 10 (64 bit).

FAQ-3164
Can I reinstall the PyroMark Q24 software on my new computer or following an operating system upgrade, or do I need to purchase a new license?

If you need to install the PyroMark Q24 software on a new computer replacing your old one, or after you reinstall or upgrade the operating system, you can reinstall the PyroMark Q24 software without purchasing a new license.

FAQ-3473