GT-Digital SARS-CoV-2 Wastewater Surveillance Assay For QIAcuity

• Multiplexed quantification of N1, N2, BCoV and PMMoV
• All CDC-NWSS compliance-required controls are included
• Manufactured following cGMP
• Wet lab-validated molecular reagent kit

The GT-Digital SARS-CoV-2 Wastewater Surveillance Assay For QIAcuity comprises a molecular reagent kit containing all primers, probes, and controls for wastewater surveillance of SARS-CoV-2 in compliance with the CDC Wastewater Surveillance Testing Method guidance for reporting to the National Wastewater Surveillance System (NWSS). Assay solutions and appropriate controls are provided for the quantification of 1) SARS-CoV-2 (N1 and N2, published by CDC); 2) Bovine Coronavirus (BCoV), a matrix recovery control (also called a process control) biologically similar to SARS-CoV-2; and 3) Pepper Mild Mottle virus (PMMoV), a fecal indicator that can estimate the human fecal content in a sample.

The GT-Digital SARS-CoV-2 Wastewater Surveillance Assay For QIAcuity provides a fast, easy-to-use system with very high accuracy, sensitivity, specificity and reproducibility.

• High target specificity for each reaction component
• Easy to set reliable thresholds with a strong separation between positive and negative partition populations
• Clean non-template controls, serial dilutions of SARS-CoV-2 pre-quantified RNA
• Robust linear dynamic range with a high degree of linearity of response across dilutions
• High accuracy and reproducibility across replicates
• Ultra-sensitivity with less than two copies detected per reaction

Accurate testing requires quantification methods specifically designed for wastewater samples. The GT-Digital SARS-CoV-2 Wastewater Surveillance Assay For QIAcuity comprises a molecular reagent kit containing all primers, probes and controls for wastewater surveillance of SARS-CoV-2 in compliance with the CDC Wastewater Surveillance Testing Method guidance for reporting to the National Wastewater Surveillance System (NWSS). Digital PCR can measure low concentrations of SARS-CoV-2 RNA present in wastewater with high precision and shows a strong correlation with qPCR results.

Wastewater-based epidemiology can be greatly enhanced by using digital PCR in the workflow, particularly for applications requiring high precision and sensitivity.
Fully validated standard operating procedures include:

• Collecting and shipping samples to the lab
• Processing samples, including viral concentration
• Detecting and analyzing the samples
• Reporting your data to NWSS

Using this kit in conjunction with the QIAcuity dPCR instrument, users can monitor SARS-CoV-2 levels and normalize for changes in viral recovery caused by the variable and complex nature of wastewater, as well as normalize for human waste input, which can change over time due to dilution and variations in human behavior (travel, tourism, etc.).