QIAwave DNA Blood & Tissue Kit

The QIAwave DNA Blood & Tissue Kit is an eco-friendlier version of our standard DNeasy Blood & Tissue Kit. The QIAwave Kit uses 63% less plastic and 42% less cardboard than our standard kit and waste tubes made from 100% post-consumer recycled plastic that you can reuse throughout the procedure. QIAwave buffers also come as concentrates, reducing the amount of plastic by 74–80% per bottle. To save paper, there are no printed protocols in the kit. You can download the protocols you need from the Resources section below or by scanning the QR code inside the kit box. So, while the packaging and components of our QIAwave Kit may look different, it’s as easy to use the QIAwave DNA Blood & Tissue Kit and the chemistry and performance are identical.

In partnership with My Green Lab, we've also assessed the environmental impact of this kit. My Green Lab ACT (accountability, consistency, and transparency) environmental impact factor labels are designed to evaluate and score products on several sustainability criteria, including:

• manufacturing
• responsible chemical management
• sustainable content within products and packaging materials
• disposal of the packaging at the end of life

Products are scored from 1 to 10 except for energy and water consumption, which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental impact. You can view the ACT labels for this kit in the Resources section below.

The QIAwave Kit uses silica-based spin-columns for purification, and most samples can be lysed directly with proteinase K. This means:

• No mechanical disruption
• No organic extraction
• No alcohol precipitation

The standard protocol for this kit allows you to extract total DNA from animal blood and tissue samples. We’ve also developed protocols for other samples types to ensure you get reproducible quantities of high-quality DNA, whether you’re working in life science, genotyping or veterinary pathogen research. You can also automate your sample extraction on the QIAcube Connect.

The performance between the QIAwave DNA Blood & Tissue Kit and the DNeasy Blood & Tissue Kit is identical because the chemistry is the same. We’ve also shown that both kits outperform competitors’ kits (see figure “ QIAwave DNA Blood & Tissue Kit performance”).
The standard protocol of the QIAwave DNA Blood & Tissue Kit gives high yields of total DNA from animal blood and tissue samples (see table "Typical DNA yields from animal tissues using QIAwave DNA Blood & Tissue " and figure  "DNA yields"). However, we also offer optimized protocols to ensure high yields from nonstandard samples, such as:

• Animal hair
• Cultured cells
• Gram-positive and -negative bacteria
• Yeast
• Insects
• Other sample types

Typical yields from animal tissues using QIAwave DNA Blood & Tissue Kit

The QIAwave DNA Blood & Tissue Kit rapidly purifies total DNA (e.g., genomic, mitochondrial, and pathogen) from different sample types, including fresh or frozen animal tissues and cells, blood or bacteria.
The membrane combines the binding properties of a silica-based membrane with simple microspin technology. The DNA adsorbs to the membrane in the presence of high concentrations of chaotropic salt, which removes water from hydrated molecules in solution. The buffer conditions allow specific adsorption of DNA to the silica membrane and removal of contaminants and enzyme inhibitors.
The entire purification process does not require phenol or chloroform extraction or alcohol precipitation and involves minimal handling, which means you can easily process multiple samples simultaneously.

The QIAwave DNA Blood & Tissue Kit purifies DNA using DNeasy Mini Spin-Columns containing a silica-membrane. This makes the procedure fast and reproducible while eliminating manual and organic extraction or alcohol precipitatoin (see flowchart " QIAwave DNA Blood & Tissue Kit procedure").

Four simple steps to purify high-quality DNA:

1. Lyse samples in proteinase K. The buffering conditions provide optimal DNA-binding conditions.
2. Load the lysate onto the DNeasy Mini Spin Column.
3. Centrifugate samples. The DNA is selectively bound to the membrane as contaminants pass through.
4. Remaining contaminants and enzyme inhibitors are then removed in two efficient wash steps, and DNA is then eluted in water or buffer, ready for you to use.

QIAwave buffers come as concentrates that you can easily reconstitute by adding water and/or ethanol; please check the handbook for details. QIAwave DNeasy Mini Spin Columns and Waste Tubes come in individual bags and need to be preassembled before you start the protocol. This takes a little extra time, but it does reduce plastic waste.
If you have too many samples and not enough time to process them manually, you can automate the purification process using the DNeasy Mini Spin Columns on the QIAcube Connect.

QIAwave DNA Blood & Tissue provides high-quality DNA, ready to use in all downstream assays, including applications in:

• Life science research
• Livestock breeding
• Pedigree genotyping
• Veterinary pathogen research
• Routine applied testing