PML-RARA bcr1 Kit is a ready-to-use kit for the quantification of PML-RARA type bcr1 fusion transcripts using real-time PCR. The ipsogen
PML-RARA bcr1 Kit is based on the amplification and detection of specific PML-RARA bcr1 transcripts, relative to ABL control gene expression, in total RNA extracted from bone marrow or peripheral blood samples in a subgroup of acute myeloid leukemia (AML) patients diagnosed with M3 cytomorphology and t(15;17)(q22;q21) translocation, with a breakpoint into PML intron 6 (see figures PML-RARA bcr1 gene transcript
and ABL control gene transcript
). The kit provides high levels of specificity, sensitivity, and reproducibility. Each ipsogen
PML-RARA bcr1 Kit provides 5 standard dilutions for PML-RARA bcr1 fusion gene and 3 standard dilutions for ABL. Use of the standards enables accurate quantification of transcripts. The technology used for quantification of PML-RARA bcr1 has been standardized according to Europe Against Cancer (EAC) recommendations and the ipsogen
PML-RARA bcr1 Kit uses this validated technology to calibrate results.