SARS-CoV-2 Whole Genome Sequencing Services

S_9900_GenomicsCovid19_Gi1155828583
Accelerating your SARS-CoV-2 research through the pandemic
Combatting the COVID-19 pandemic requires greater insights into the molecular epidemiology of SARS-CoV-2 to trace existing outbreaks, study viral evolution and advance vaccine and drug-development research.

QIAGEN Genomic Services provides a comprehensive Sample-to-Insight SARS-CoV-2 Whole Genome Sequencing Service to support your research. Whether you’re studying strain evolution or performing phylogeographic or metagenomic analyses, Genomic Services is the right partner for providing impactful insights and helping accelerate your COVID-19 research. This service uses technology especially designed for SARS-CoV-2 research.

Workflow

  1. RNA isolation
    Viral RNA is isolated using the QIAamp Viral RNA Kit.
  2. RNA QC
    The viral load is determined using a proprietary qPCR protocol, using primer sequences specific to N1/N2 regions of SARS-CoV-2 based on the publication of the US Center of Disease Control and Prevention (CDC).
  3. SARS-CoV-2 library preparation and sequencing
    Library preparation is performed by a targeted amplification of viral nucleic acid with the QIAseq SARS-CoV-2 Primer Panel followed by library preparation with the QIAseq FX DNA Library Prep Kit. After Library QC, library pools are sequenced on Illumina sequencing instruments.
  4. Data analysis
    Data is analyzed using QIAGEN CLC Genomics Workbench (version 20.0.2) and QIAGEN CLC Genomics Server (version 20.0.2). High-quality trimmed reads are mapped to the Wuhan-Hu-1 (GenBank: MN908947.3) genome. Variant detection down to 1% is then reported from the read alignments and a consensus sequence computed from the read alignments.

 

 

Contact Genomic Services to learn more about the options available to you and consult with experts about solutions for your projects.

  • Main Image Navi