miRNeasy 96 Advanced QIAcube HT Kit

For automated purification of total RNA, including miRNA, from serum and plasma samples

S_1084_5_GEN_V2

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miRNeasy 96 Advanced QIAcube HT Kit (5)

Cat. No. / ID:  217261

For 480 preps: RNeasy 96 plates, RNase-free water, buffers
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KitAccessories
miRNeasy 96 Advanced QIAcube HT
QIAcube HT Plasticware
The miRNeasy 96 Advanced QIAcube HT Kit is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Phenol-free miRNA isolation from human, mouse or rat serum and plasma samples
  • Simple and reliable automated processing for time and cost savings
  • High-quality miRNA ready for use in any downstream application
  • Consistent miRNA yields from small amounts of starting material

Product Details

The miRNeasy 96 Advanced QIAcube HT Kit is designed for automated high-throughput purification of cell-free total RNA – primarily miRNA and other small RNA – from small volumes of serum and plasma on the QIAcube HT. Using proven RNeasy silica-membrane technology in a convenient 96-well format, contaminants and inhibitors are removed to yield high-quality RNA ready for downstream analysis.

Performance

The miRNeasy 96 Advanced QIAcube HT Kit is the medium- to high-throughput version of the miRNeasy Serum/Plasma Advanced Kit and provides the same yield and quality of RNA using phenol-free isolation technology (see figures  Performance comparable with manual kit using equal volumes) and  Performance comparable with manual kit using equal amounts of RNA). The miRNeasy 96 Advanced QIAcube HT Kit efficiently isolates total RNA, including miRNA, from human and animal serum and plasma samples from 50 µl to 200 µl ( Processing different amounts of plasma). The kit is compatible with the majority of blood collection devices (see figure  Compatibility with different blood collection tubes). The phenol-free protocol uses easy-to-automate spin column technology in 96-well format and minimize hands on time. Unlike kits from other suppliers, the miRNeasy 96 Advanced QIAcube HT Kit combines ease-of-use and a phenol-free protocol without any compromise on RNA quality or yield (see figure  Comparable or better yields and performance).

See figures

Principle

The miRNeasy 96 Advanced QIAcube HT Kit enables automated purification of cell-free total RNA – primarily miRNA and other small RNA, from 20–200 µl of human or animal plasma using the QIAcube HT instrument. Selective binding properties of a silica membrane are combined with a high-throughput 96-well format for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument. The kit combines guanidine-based lysis of samples, an inhibitor removal step and silica-membrane-based purification of total RNA.

The system provides two protocols, a transfer protocol to move the lysate from the protein precipitation step to the RNeasy 96 plate and the cell-free RNA isolation protocol to extract miRNA from the lysate. The procedures yield high-quality RNA that performs well in downstream applications .

Procedure

Lysis is performed manually under highly denaturing conditions with guanidine thiocyanate to immediately inactivate RNases and ensure purification of intact RNA (see figure  miRNeasy 96 Advanced QIAcube HT Kit procedure.)

After the run is started, isopropanol is added to the RNA-containing supernatant to provide appropriate binding conditions for all RNA molecules from approximately 18 nucleotides (nt) upwards. The samples are afterwards transferred (manually or automated) to the wells of the RNeasy 96 plate. Total RNA binds and contaminants are efficiently washed away. High-quality RNA is then eluted in RNase-free water, ready for use in any downstream application.

See figures

Applications

The miRNeasy 96 Advanced QIAcube HT Kit isolates total cell-free and exosomal RNA from plasma or serum. Downstream applications include:

  • Biomarker discovery
  • Expression profiling
  • Next-generation sequencing
  • Quantitative, real-time RT-PCR
  • Microarray analysis

Supporting data and figures

Specifications

FeaturesSpecifications
Number of preps per run24–96 samples (processed in increments of 8)
Input volumeUp to 200 µl
Elution volume80–100 µl
Run time96 samples in approximately 93 minutes; 24 samples in approximately 54 minutes

Resources

Operating Software (1)
Technical Information and Important Notes (1)
Quick-Start Protocols (1)
Certificates of Analysis (1)

FAQ

Can I run the QIAcube HT with the QIAxtractor software or vice versa?

No, the two software applications are not interchangeable.

 

Additionally, the QIAcube HT and QIAxtractor software cannot be installed on the same computer. The QIAcube HT software versions is v4.17 or higher, while the QIAxtractor software version is v4.16 or lower.

FAQ ID -9102
For what batch size is the QIAcube HT Plasticware designed?
The QIAcube HT Plasticware content is calculated for use with the extraction protocol working in batches of 96 samples. If running smaller batches and/ or the transfer protocol, additional consumables are required. 
FAQ ID - 4010
Can I upgrade a Corbett CAS-1820 to a QIAcube HT?

No, this upgrade is not possible.

FAQ ID -9104
What file is required for reporting a QIAcube HT or QIAxtractor issue?

The Support Packages are important when reporting an issue to QIAGEN Technical Services. Upon completion of each run, the software automatically saves a support package.

 

To retrieve a support package, click Help in the QIAcube HT or QIAxtractor software and select Choose support package. In the new windows, select the support package that corresponds to the issue and send it to QIAGEN Technical Services.

 

FAQ ID -9100
I am getting vacuum errors on my QIAcube HT. What should I do?

1. Check the vacuum tubing to make sure it is not pinched or obstructed in some way. Sometimes the tubing can become pinched against the bench top when the vacuum station is placed too far from the instrument.

Waste bottle tubing

2. Make sure that the channeling adapter is properly placed. Check if the carriage is moving the plate completely from the waste chamber position to the elution chamber position. If the calibration seems incorrect, the instrument may need to be re-taught at this position.

Adaptors

3. When using less than a full plate, make sure all the unused columns are covered with an adhesive seal.

Also, make sure each column of 8 samples is full. When processing a number of samples that results in < 8 samples per column, use water as a substitute in the empty positions until the column is filled. This ensures even vacuum across all unsealed well positions.

4. Make sure correct plates are used for the selected protocol.

5. When the vacuum is on, please listen for any hissing noise from the tubing that is coming down to the waste container. This could indicate leaks in the tubing.

If the issue persists, please contact QIAGEN Technical Service.



FAQ ID -9106
What do I do if the QIAcube HT or QIAxtractor software runs in virtual mode while the instrument is connected to the computer?

Make sure the QIAcube HT or QIAxtractor software uses the same COM port number as assigned to the QIAcube HT or QIAxtractor instrument on the computer. The COM port setting can be checked by clicking Options\Robot Setup\Select COM port in the QIAcube HT or QIAxtractor software.

 

Ensure that the cable between the instrument and the computer is securely inserted. Power on the instrument first, and then launch the QIAxtractor/QIAcube HT software. 

 

The instrument has both RS-232 (serial) and USB interfaces for computer connection. Try an RS-232 cable if the communication cannot be established between the instrument and computer with a USB cable.

 

If the issue persists, please contact QIAGEN Technical Services.

 

FAQ ID -9101