artus HI Virus-1 RT-PCR Kits CEFor quantitative detection of HIV-1 specific RNA using real-time PCR
The artus HI Virus-1 RG RT-PCR Kit and the artus HI Virus-1 QS-RGQ Kit are ready-to-use molecular detection kits for real-time RT-PCR on Rotor-Gene Q instruments. The kits provide all necessary reagents optimized for reliable HIV-1 RNA detection and quantitation for in vitro diagnostic use. The artus HI Virus-1 RG RT-PCR Kit uses manual sample preparation and assay setup techniques. The artus HI Virus-1 QS-RGQ Kit is part of the QIAsymphony RGQ, the complete automated system from sample to pathogen detection, and is not for use with manual assay setup.
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artus HI Virus-1 RT-PCR Kits CE are intended for in vitro diagnostic use in Europe.
Product Details
Performance
To ensure highest sensitivity, artus HI Virus-1 Kits have been optimized to detect low numbers of HIV-1 RNA. The analytical sensitivity of the artus HI Virus-1 QS-RGQ Kits is 76.4 IU/ml in consideration of the purification and assay setup using the QIAsymphony RGQ system (see figure "Highly sensitive detection of HIV-1 RNA"). (1 IU/ml corresponds to 0.45 copies/ml for detection of HIV-1 RNA on the Rotor-Gene Q. The conversion factor is an approximation based on an average factor across the assay's dynamic range.) For highest specificity, validation of the artus HI Virus-1 Kits was carried out using various HIV-1 isolates, including all group M subtypes A–H, and related pathogens.
Principle
The artus HI Virus-1 RG RT-PCR Kit and the artus HI Virus-1 QS-RGQ Kit are based on the amplification and simultaneous detection of a specific region of the HIV-1 genome using real-time RT-PCR. The kits provide high levels of specificity, sensitivity (see figure "Highly sensitive detection of HIV-1 RNA"), and reproducibility over a broad linear range.
Procedure
artus HI Virus-1 RT-PCR Kits provide all necessary reagents optimized for reliable HIV-1 RNA detection and quantitation. Simply add template RNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook. Complete automated system from sample to HIV-1 detectionThe QIAsymphony RGQ workflow solution for HIV-1 detection comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and the artus HI Virus-1 QS-RGQ Kit on the Rotor-Gene Q. The system enables reliable pathogen detection with a complete CE-IVD-compliant workflow (see figure "Integrated QIAsymphony RGQ system for HIV-1 detection"). Recommendations for manual viral RNA purificationThe artus HI Virus-1 RG RT-PCR Kit is validated for use with viral RNA purified from human plasma using the CE-marked QIAamp DSP Virus Kit.
Applications
The artus HI Virus-1 RG RT-PCR Kit enables rapid and sensitive detection and quantitation of HIV-1 RNA purified from human plasma using the QIAamp DSP Virus Kit. The artus HI Virus-1 QS-RGQ Kit is designed to be used with the QIAsymphony RGQ system, providing a complete CE-IVD-compliant workflow from sample to HIV-1 RNA detection and quantitation. Specifications
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