AdnaTest workflow
AdnaTest workflow
AdnaTest Select – Immunomagnetic cell selection with multiple tumor-associated
AdnaTest Select – Immunomagnetic cell selection with multiple tumor-associated
AdnaTest Detect – Multiplex PCR of various cancer-associated tumor markers
AdnaTest Detect – Multiplex PCR of various cancer-associated tumor markers
AdnaTest BreastCancerDetect results
AdnaTest BreastCancerDetect results
AdnaTest workflow AdnaTest BreastCancerSelect enables the immunomagnetic enrichment of tumor cells via epithelial and tumor-associated antigens. Antibodies against epithelial and tumor-associated antigens are conjugated to magnetic beads (Dynabeads) for labeling of tumor cells in peripheral blood. Labeled cells are extracted by a magnetic particle concentrator (AdnaMag-L and AdnaMag-S) and are subsequently lysed. The results of the AdnaTest are generate within 8 hours.
AdnaTest Select – Immunomagnetic cell selection with multiple tumor-associated In the first step, the CTCs in the blood are enriched (AdnaTest Select). This is achieved using antibody-coated magnetic particles (beads). Several antibodies are used, which bind with high specificity and affinity to the corresponding cancer cells. The enriched cells are lysed and subsequently purified several times to extract mRNA.
AdnaTest Detect – Multiplex PCR of various cancer-associated tumor markers In a second step the enriched cells are examined by RT-PCR for tumor-associated expression patterns. The mRNA strands are reverse transcribed into cDNA. Subsequently, several tumor-associated markers can be amplified using multiplex PCR and visualized.
AdnaTest BreastCancerDetect results The first lane shows the DNA size standard (DNA-Ladder). Sample 1 is positive for GA733-2, Muc-1 and Her-2. Samples 3, 4 and 5 are positive for GA733-2. Muc-1 in sample 2 is negative. The only band detected in sample 6 is for GA733-2. Actin is detected in samples 1 to 6. The PCR negative (C-) and positive control (C+) are shown in the last two lanes (Agilent 2100 Bioanalyzer).