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UDGase (500 U)
Cat. No. / ID: EN19-050
500 μL of UDGase (1 U/μL), 1 mL of 10x UDGase Reaction Buffer. Storage temperature: Keep at -20°C without a defrost cycle. The enzyme can be irreversibly inactivated by incubation at 95°C for 10 minutes.
Quantity
500 U
2500 U
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The UDGase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Need bulk, customized or optimized products for commercial purposes? We also offer support with logistics, compliance and more. Reach out to cooperate with QIAGEN Strategic Partnerships & OEM
Features
- Modified recombinant enzyme over-expressed in E. coli
- Sensitive mutant is irreversibly inactive after heating
- Catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA but not from RNA or oligonucleotides
- Active over a broad pH range (optimal at pH 8.0)
- Shows no activity on RNA or oligonucleotides
Product Details
UDGase catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single-stranded or double-stranded DNA. The enzyme shows no activity on RNA or oligonucleotides.
It is supplied with 20 mM Tris-Cl, pH 8.0, 0.1 M NaCl, 0.1 mM EDTA , 1 mM DTT, 0.1% stabilizer and 50% glycerol.
One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from uracil-containing dsDNA. The activity is measured by the release of [3H]-uracil in a 50 μL reaction containing 0.2 μg DNA in 30 minutes at 37°C.
Performance
| Assay | Specification |
| Unspecific DNA nucleases | None detected |
Principle
E. coli Uracil DNA Glycosylase catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single-stranded or double-stranded DNA. The enzyme shows no activity on RNA or oligonucleotides.
Procedure
Quality Control
UDGase is tested for degradation of PCR product containing dUTPs. The product is free of unspecific DNA nucleases.
Usage
Treatment of 0.1 µg of uracil-containing DNA with 1 unit of UDG for 10 minutes at 37°C renders the DNA incapable of being copied by DNA polymerase. The enzyme can be irreversibly inactivated by incubating at 95°C for 10 minutes.
Applications
This is used for applications such as:
- Elimination of carry-over contamination in PCR
- Probe for protein-DNA interaction studies
- Glycosylase mediated single nucleotide polymorphism detection (GMPD)
- Molecular cloning of PCR products
Resources
Kit Handbooks (1)
Safety Data Sheets (1)
Certificates of Analysis (1)