Discover QIAcuity digital PCR systems as OEM ̶ for your commercial assay manufacturing
Do you want to take your commercial PCR assay to the next level? Imagine keeping the familiarity and ease of qPCR but gaining the higher sensitivity, precision and absolute quantification of digital PCR (dPCR) without prolonging the time to market for your commercial assay. Discover QIAcuity, our fully integrated nanoplate-based digital PCR system. With OEM by QIAGEN, you can provide your customer and R&D lab scientists with an enhanced digital PCR experience by integrating the QIAcuity digital PCR systems into your workflows.
Learn more about QIAcuity
Nanoplates-based digital PCR system vs. qPCR
Integrate dPCR into your workflow
Digital PCR for manufacturers is about accelerating your commercial assay innovation. When you need speed, sensitivity, specificity and ease of use, integrate QIAcuity to your workflow and bring advanced dPCR technologies to your lab and your customers.
Digital PCR vs. qPCR
When addressing a “needle in a haystack” challenge, such as identifying a weak genetic signal in a strong background, partitioning the sample into many individual reactions provides increased throughput, enhanced sensitivity and the capability of absolute quantification.
Nanoplate-based digital PCR system for diverse applications
Nanoplate-based digital PCR technology uses the procedure of end-point PCR but splits into many individual reactions. After PCR, the amplification target is detected by measuring the fluorescence – of either sequence-specific DNA probes or intercalating dyes. Imagine the benefits of automation and dPCR available to your customers in combination with the familiarity of your innovative PCR assay. QIAcuity, QIAGEN's fully integrated nanoplate-based digital PCR system, has been designed to offer these benefits:
- Fixed partitions
- Sealed nanoplates
- Faster readout
- Easy-to-use, plates
- Front-end automation
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Quality at scale- Throughput from 96 to 1,248 samples per day
- Three instrument configurations
- Up to two integrated thermocyclers
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Multiplexing and quick results- From sample to result in under two hours
- Up to five detection channels
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Sensitivity- Partition number and input volume per well depends on plate configuration
QIAGEN experts share their dPCR technologies expertise
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Introducing the QIAcuityIn this on-demand webinar, Dr. Andreas Missel covers fundamental aspects for successful digital PCR assay performance. He shares insights into templates, concentrations, quantification and analysis interpretation. -
The advantages of partitioningDr. Daniel Heinz Löfgren reviews the power of partitioning. The more partitions, the higher the resolutions and sensitivity. Additionally, a higher volume of diluted sample can still be analyzed. Watch the on-demand webinar. -
Our digital PCR system portfolioIn this on-demand webinar, Dr. Gerald Schock reviews QIAGEN’s easy-to-use, fully integrated walkway instrument options and plate configurations as a viable dPCR solution to overcome speed and throughput bottlenecks.
Dive deeper into digital PCR on the QIAcuity
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QIAcuity FAQ blogRead our blog post for a summary of our webinar introducing a successful dPCR assay on the QIAcuity instrument and answers to the top 10 questions asked. -
Experiment setup guideLearn how to set up dPCR experiments, from assay design guidance, handling and storage, sample preparation to quantification and analysis for diverse applications.
QIAcuity instruments – Features and specifications
| QIAcuity One | QIAcuity Four | QIAcuity Eight | |
|---|---|---|---|
| Plates processed | 1 | 4 | 8 |
| Detection channels (multiplexing) | 2 or 5 | 5 | 5 |
| Thermocycler(s) | 1 | 1 | 2 |
| Time to result | Under 2h | First plate under 2 h Every ~60 min a following plate |
First plate under 2 h Every ~30 min a following plate |
| Throughput (samples processed in a work day) | Up to 384 (96-well) Up to 96 (24-well) |
Up to 672 (96-well) Up to 168 (24-well) |
Up to 1248 (96-well) Up to 312 (24-well) |
Nanoplates – Features and specifications
| Specifications | Applications | |
|---|---|---|
| Nanoplate 26K 24-well | 24-well × approx. 26,000 partitions |
Rare mutation detection, liquid biopsy, pathogen detection, etc. |
| Nanoplate 8.5K 24-well | 24-well × approx. 8,500 partitions |
CNV determination, NGS library quantification, genome edit detection, etc. |
| Nanoplate 8.5K 96-well | 96-well × approx. 8,500 partitions |
CNV determination, NGS library quantification, genome edit detection, etc. |