- 快速、高效的破碎各种类型的样本
- 一次性钻头有助于消除交叉污染
- 一次性钻头节省实验时间
- 透明钻头确保破碎过程可监控
- 与QIAGEN样本制备技术无缝整合
TissueRuptor是一款手持式匀浆器,可快速、有效、灵活的对人类、动物和植物样本进行破碎,适用于多种下游应用。TissueRuptor使用透明的一次性钻头,可避免交叉污染,并且可以直观的监控样本的破碎过程。此外,使用一次性钻头可以节省每次处理样本之后的清洁时间。
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Cat. no.
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TissueRuptor II (230 V, 50/60 Hz, EU/CH)
Handheld rotor–stator homogenizer, 230 V, 50/60 Hz (for Europe [excluding UK and Ireland]), 5 TissueRuptor Disposable Probes
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9002756
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TissueRuptor Disposable Probes (25)
25 nonsterile plastic disposable probes for use with the TissueRuptor II (and the old TissueRuptor)
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990890
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The TissueRuptor is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Reproducible purification of high-quality genomic DNA.|Efficient disruption and homogenization of animal tissues.|Successful purification of phosphoproteins.|High performance results in PCR.|Pure RNA with high RIN values.|
Frozen liver and kidney samples (30 mg each) were disrupted in lysis buffer at full speed for 30 seconds using the TissueRuptor. Genomic DNA was purified using the AllPrep DNA/RNA Mini Kit and analyzed by agarose gel electrophoresis. M: markers.|The following tissues were disrupted at full speed for 30 seconds using the TissueRuptor: frozen kidney, liver, and spleen (10 mg each); frozen lung (250 mg); stabilized heart, muscle, and brain (10 mg each). Samples were either disrupted in lysis buffer (kidney, liver, spleen, lung, heart, and muscle) or QIAzol Lysis Reagent (brain). Total RNA was purified using the RNeasy Plus Mini Kit (RNeasy Plus), the RNeasy Midi Kit (RNeasy Midi), the RNeasy Fibrous Tissue Mini Kit (RNeasy Fibrous), or the RNeasy Lipid Tissue Mini Kit (RNeasy Lipid). Purified RNA was analyzed by formaldehyde agarose gel electrophoresis. The gels show the high yields and quality of the RNA following disruption using the TissueRuptor.|Frozen rat liver and brain (approximately 30 mg each) were disrupted at medium speed for 30–60 seconds in 350 μl PhosphoProtein Lysis Buffer. The homogenized samples were then diluted with 1500 μl PhosphoProtein Lysis Buffer and incubated at 4°C for 30 minutes, with brief vortexing every 10 minutes. Phosphoproteins were purified using the PhosphoProtein Purification Kit and detected by western blotting. [A] Transfer membrane stained with Ponceau S, and [B] western blot. M: markers; FT: flow-through; E3: elution fraction 3.|[A] Liver and [B] heart samples (25 mg each, frozen) were disrupted with the TissueRuptor or a traditional rotor–stator homogenizer and lysed for 1 hour with proteinase K, or lysed overnight with proteinase K without sample disruption. DNA was purified using the QIAamp DNA Mini Kit. Eluates (5 μl) were subjected to PCR using HotStarTaq DNA Polymerase and primers specific for the 18S ribosomal RNA gene. M: GelPilot Mid Range Ladder.|Frozen liver samples (30 mg each) were disrupted at full speed for 30 seconds using either the TissueRuptor with disposable probes or a traditional rotor–stator homogenizer with a steel generator probe. Total RNA was purified using the RNeasy Plus Mini Kit and analyzed on the Agilent 2100 Bioanalyzer. The high RNA Integrity Number (RIN) indicates the high quality of the RNA.|
Principle
基因分型、基因表达分析和蛋白质组学研究均要求对生物样本进行高效破碎,以确保获得高产的DNA、RNA和蛋白质。TissueRuptor可快速、彻底的破碎样本,彻底释放生物分子,并可同时对样本进行匀质化,协助后续使用QIAGEN试剂盒进行纯化。
QIAGEN提供从样本收集到DNA、RNA和蛋白质纯化的完整解决方案,TissueRuptor是其中关键的组成部分。优化的实验方案整合了样本破碎和生物分子纯化,提供高效的工作流程。此外,多种自动化解决方案可用于低至高通量的生物分子纯化和分析。
QIAGEN的RNAlater RNA Stabilization Reagent可稳定RNA,Allprotect Tissue Reagent可稳定DNA、RNA和蛋白质。TissueRuptor可轻松破碎使用以上试剂采集的组织样本。
<>与QIAGEN破碎仪器兼容的QIAGEN纯化试剂盒 >
| RNA |
容易裂解的组织 |
RNeasy Kits |
| RNeasy Plus Kits |
| RNeasy Protect Kits |
| RNA |
富含纤维的组织 |
RNeasy Fibrous Tissue Kits |
| RNA |
各种类型组织 |
RNeasy Lipid Tissue Kits |
| RNeasy Universal Tissue Kits |
| RNA |
植物组织 |
RNeasy Plant Mini Kit |
| RNA |
酵母 |
RNeasy Kits |
| RNA |
细菌 |
RNeasy Protect Bacteria Kits |
| microRNA |
各种类型组织 |
miRNeasy Kits |
| DNA |
人类组织 |
QIAamp DNA Kits |
| DNA |
动物组织 |
DNeasy Blood & Tissue Kits |
| 蛋白质 |
组织 |
Qproteome Mammalian Protein Prep Kit |
| 磷蛋白 |
组织 |
PhosphoProtein Purification Kit |
| 糖蛋白 |
组织 |
Qproteome Glycoprotein Kits |
| DNA和RNA |
组织 |
AllPrep DNA/RNA Kits |
| DNA、RNA和蛋白质 |
组织 |
AllPrep DNA/RNA/Protein Mini Kit |
Procedure
TissueRuptor是一款手持式设备,带有TissueRuptor一次性钻头。钻头的刀片高速旋转,通过搅动和机械剪切作用,同时破碎和匀质化样本。适用的样本类型包括人类、动物和植物组织(对于难破碎的植物组织,建议使用TissueLyser II)。通常只需全速破碎30秒,即可释放起始样本中的核酸或蛋白质。
样本破碎完成后,可丢弃TissueRuptor的一次性钻头,使用新钻头破碎下一个样本。无需清洗、再使用同一钻头,因此可节省时间,避免交叉污染。此外,TissueRuptor使用一次性透明钻头,可以直观的监控样本破碎过程。
Applications
TissueRuptor可高效破碎并匀浆人类、植物和动物组织样本,包括血块。使用QIAGEN试剂盒对RNA、DNA、总核酸或蛋白质进行纯化。
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Download Safety Data Sheets for QIAGEN product components.
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For low-throughput disruption of biological samples using disposable probes
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Images
Reproducible purification of high-quality genomic DNA.
Frozen liver and kidney samples (30 mg each) were disrupted in lysis buffer at full speed for 30 seconds using the TissueRuptor. Genomic DNA was purified using the AllPrep DNA/RNA Mini Kit and analyzed by agarose gel electrophoresis. M: markers.
Efficient disruption and homogenization of animal tissues.
The following tissues were disrupted at full speed for 30 seconds using the TissueRuptor: frozen kidney, liver, and spleen (10 mg each); frozen lung (250 mg); stabilized heart, muscle, and brain (10 mg each). Samples were either disrupted in lysis buffer (kidney, liver, spleen, lung, heart, and muscle) or QIAzol Lysis Reagent (brain). Total RNA was purified using the RNeasy Plus Mini Kit (RNeasy Plus), the RNeasy Midi Kit (RNeasy Midi), the RNeasy Fibrous Tissue Mini Kit (RNeasy Fibrous), or the RNeasy Lipid Tissue Mini Kit (RNeasy Lipid). Purified RNA was analyzed by formaldehyde agarose gel electrophoresis. The gels show the high yields and quality of the RNA following disruption using the TissueRuptor.
Successful purification of phosphoproteins.
Frozen rat liver and brain (approximately 30 mg each) were disrupted at medium speed for 30–60 seconds in 350 μl PhosphoProtein Lysis Buffer. The homogenized samples were then diluted with 1500 μl PhosphoProtein Lysis Buffer and incubated at 4°C for 30 minutes, with brief vortexing every 10 minutes. Phosphoproteins were purified using the PhosphoProtein Purification Kit and detected by western blotting. [A] Transfer membrane stained with Ponceau S, and [B] western blot. M: markers; FT: flow-through; E3: elution fraction 3.
High performance results in PCR.
[A] Liver and [B] heart samples (25 mg each, frozen) were disrupted with the TissueRuptor or a traditional rotor–stator homogenizer and lysed for 1 hour with proteinase K, or lysed overnight with proteinase K without sample disruption. DNA was purified using the QIAamp DNA Mini Kit. Eluates (5 μl) were subjected to PCR using HotStarTaq DNA Polymerase and primers specific for the 18S ribosomal RNA gene. M: GelPilot Mid Range Ladder.
Pure RNA with high RIN values.
Frozen liver samples (30 mg each) were disrupted at full speed for 30 seconds using either the TissueRuptor with disposable probes or a traditional rotor–stator homogenizer with a steel generator probe. Total RNA was purified using the RNeasy Plus Mini Kit and analyzed on the Agilent 2100 Bioanalyzer. The high RNA Integrity Number (RIN) indicates the high quality of the RNA.
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