text.skipToContent text.skipToNavigation

QIAseq LNA Library Quant Kit

For quantification of mRNA, whole transcriptome, miRNA or DNA libraries for Illumina NGS platforms
  • Highly accurate quantification using a probe-based system
  • No need to correct for library fragment size
  • Ensures equal loading of samples and optimal sequencing results
  • High-performance LNA probe and LNA primers designed by our LNA experts
  • Available in tubes or ready-to-use PCR plates for easy setup and high reproducibility

The QIAseq LNA Library Quant Kit enables highly accurate quantification of RNA or DNA libraries prior to NGS on Illumina platform through an easy and rapid workflow. The kit uses a dual-labeled hydrolysis probe to provide absolute quantification, so you can pool your sequencing libraries optimally to ensure maximal NGS reads and data analysis power.



Need a quote for your research project or would you like to discuss your project with our specialist team? Contact Us
Cat No./ID: 339910
QIAseq LNA Library Quant Kit
Dual-labeled hydrolysis probe-based PCR array for library quantification prior to NGS
0
A unique probe-based system for library quantification.
The QIAseq LNA Library Quant Kit is the only system for quantification of libraries for Illumina sequencing that is based on absolute quantification using a dual-labeled hydrolysis probe. LNA primers target the P5 and P7 Illumina adaptor sequences, and the dual-labeled LNA probe targets the i7 index I primer binding site. (A) When the fluorophore and the quencher are in proximity, quenching inhibits any fluorescence signal. (B) Primer extension results in displacement and cleavage of the probe, allowing fluorescence of the fluorophore. The signal from one probe equates to one molecule, which means that quantification is based on molecule counting, so it is not necessary to correct for any variation in the size distribution of the fragments in the library.
1
A rapid, easy workflow for library quantification.
2
Accurate absolute quantification of libraries by molecule counting.
The four dilution standards (representing 10-fold dilutions of a synthetic DNA template, orange) as well as 10,000-fold dilutions of real samples (blue) are amplified and Cq values determined. The standard curve is used to convert the Cq values of the samples into concentration values. Since the reactions are based on probe cleavage, the signal from one probe equates to one molecule, so it is not necessary to correct for any variation in the size distribution of the fragments in the library.
3
Overview of the QIAseq LNA Library Quant Kit.
The LNA primers and LNA probe and are provided in a tube or in ready-to-use PCR panels for maximum ease of use and reproducibility. Highly accurate absolute quantification is obtained using a standard curve generated from four DNA standards provided in 10-fold dilutions (20 pM, 2 pM, 0.2 pM and 0.02 pM).
4
Ready-to-use PCR panels for maximum ease of use and reproducibility.
It is recommended to run triplicates of samples and the four DNA standards (template 1, 2, 3 and 4) used to generate the standard curve, as well as the no-template control (water). This plate layout is recommended when using our Library Concentration Calculator to calculate the library concentrations. Alternatively, LNA primers and LNA probe are available in tube format.
Performance

 

Principle

 

Procedure

 

您无权限查看此资源

fragment fix placeholder