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QIAseq Immune Repertoire RNA Library Kits

For human and mouse immune repertoire screening
  • Unique Molecular Indices (UMI) ensure accurate sequencing results
  • Online data analysis through GeneGlobe
  • Includes QIAseq Beads for reaction cleanup
  • Automation-friendly protocol for human or mouse samples
  • QIAseq Sample Indices ordered separately to multiplex up to 384 samples


The QIAseq Immune Repertoire RNA Library Kits use Unique Molecular Indices (UMI) with gene-specific primers to target specific RNAs for NGS sequencing. Each unique panel is carefully designed and laboratory-verified for sequencing performance with a UMI-aware alignment software for maximum sequencing performance and accurate results. The Human and Mouse T-cell Receptors Panel is used for sequencing the V(D)J region of the alpha, beta, delta and gamma genes, including the CDR3 regions. Online analysis through the GeneGlobe Data Analysis Center provides key sequencing QC metrics, as well as the frequency and identity of each clonotype sequenced.
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产品 Product no. 货号 目录价:
 
 
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    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.

QIAseq Immune Repertoire RNA Library Kits适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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快速启动实验方案 (3)
Part 1: RT primer hybridization, reverse transcription, second strand synthesis, end-repair, A-addition

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Part 2: Adapter ligation, cleanup of adapter-ligated DNA, target enrichment
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Part 3: Cleanup of target enrichment, universal PCR, cleanup of universal PCR
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