text.skipToContent text.skipToNavigation

QIAseq DNA QuantiMIZE Kits

For quantification and qualification of amplifiable DNA prior to NGS
  • Provides 2 qPCR assays dispensed onto arrays or in individual tubes
  • Quantifies and qualifies DNA that is amplifiable by PCR
  • Guides optimization of targeted enrichment conditions
  • Requires no serial dilutions
The QIAseq DNA QuantiMIZE System is a qPCR-based approach to determine quantity and quality of DNA that is amenable to amplification by targeted enrichment prior to NGS. The system has been developed for discovery, translational, and clinical researchers who need a cost-effective way to determine the optimum conditions for targeted enrichment of DNA isolated from biological samples, including FFPE. In contrast to other approaches, the system improves the likelihood of a successful NGS run by not only quantifying amplifiable DNA, but also optimizing the target enrichment conditions. 

Please note: GeneRead DNA QuantiMIZE Array Kit (product number 180642) and GeneRead DNA QuantiMIZE Assay Kit (product number 180654) will be phased out by the end of 2016. Please use QIAseq DNA QuantiMIZE Array Kit (product number 333404) and QIAseq DNA QuantiMIZE Assay Kit (product number 333414instead.

Need a quote for your research project or would you like to discuss your project with our specialist team? Contact Us
产品 Product no. 货号 目录价:
 
 
Show details
    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
Available Products
2
产品 Product no. 货号 目录价:
2 arrays in Formats A, C, D, or F; or 2 discs in Format R; or 1 array in Format E or G; QuantiMIZE Control gDNA (50 μl; GeneRead qPCR SYBR Green Mastermix (2 x 1.35 ml)
show details
333404 DNQA-002 varies
Assay 100 and Assay 200 for 400 x 25 μl reactions (400 μl) each; QuantiMIZE Control gDNA (50 μl); RNase- and DNase-free water (1 ml x 4); GeneRead qPCR SYBR Green Mastermix (1.35 ml x 4)
show details
333414 DNQC-100Y varies
Configure
产品 Product no. 货号 目录价:
 
 
Show details
    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.

GeneRead DNA QuantiMIZE Kits适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


0
GeneRead DNA QuantiMIZE产品成功定量检测DNA。
使用GeneRead DNA QuantiMIZE System定量并定性检测可扩增DNA,可从质量较差的FFPE样本中回收DNA用于NGS分析。使用GeneRead DNA QuantiMIZE System获得高品质DNA文库,成功在MiSeq上实现测序。
1
Robust performance with FFPE samples.

The QIAseq QuantiMIZE System ensures successful library yields and sequencing metrics from FFPE samples. DNA from 15 FFPE samples was isolated and the same DNA was quantified by either spectrophotometry or QuantiMIZE. A) Library yield: NanoDrop, a spectrophotometric method, has limitations in quantifying functional (amplifiable) DNA and results in inconsistent library yields. B) Specificity of reads: QIAseq DNA QuantiMIZE system reliably quantifies functional DNA input and ensures successful NGS runs with every sample.

2
GeneRead DNA QuantiMIZE System的原理。
GeneRead DNA QuantiMIZE System采用2个qPCR assays,检测20个基因位点,以确定生物样本中可扩增的DNA量。
Performance
Quantification and qualification of amplifiable DNA by the QIAseq DNA QuantiMIZE System rescues low-quality FFPE samples for NGS analysis. Use of the QIAseq DNA QuantiMIZE System resulted in high-quality libraries that were successfully sequenced on a MiSeq (see Successful quantification and qualification of DNA by the GeneRead DNA QuantiMIZE system). 
Principle
QIAseq DNA QuantiMIZE System utilizes 2 qPCR assays that interrogate 20 genomic loci to determine the amounts of amplifiable DNA in biological samples. The assays, Assay 100 and Assay 200, generate 100 and 200 bp amplicons, respectively. QuantiMIZE Kits use the CT values of Assay 100 and Assay 200 for both the Control samples and the Case samples to quantify and qualify DNA (see Principle of the GeneRead DNA QuantiMIZE System).  
Procedure
Genomic DNA extracted from biological samples is mixed with the supplied mastermix. This mixture is then aliquoted onto arrays, if the array kits are used, or mixed with Assay 100 and Assay 200 before being aliquoted onto arrays, if the assay kits are used. PCR is performed and the raw CT values are exported to a web-based data analysis module, which will quantify and qualify DNA as well as provide guidance on DNA amounts and PCR cycles needed to optimize targeted enrichment conditions. 
Applications
QIAseq DNA QuantiMIZE Kits can be used to quantify and qualify any DNA sample. They are especially beneficial for low‑quality DNA samples, such as FFPE (see Robust performance with FFPE samples). Additionally, the kits can be used to optimize the conditions needed for successful targeted enrichment of genomic regions from any DNA sample, including low-quantity DNA samples, such as FFPE, and low-quantity DNA samples, such as fine-needle aspirates.

您无权限查看此资源

试剂盒操作手册 (2)
New version – For advanced qualification and quantification of amplifiable genomic DNA for targeted enrichment prior to next-generation sequencing

显示更多
New version – For advanced qualification and quantification of amplifiable genomic DNA for targeted enrichment prior to next-generation sequencing (assay format)

显示更多
分析软件 (4)
Excel-based data analysis template for use with QuantiMIZE assay kits for 72-well plates
显示更多
Excel-based data analysis template for use with QuantiMIZE array and assay kits for 96-well plates
显示更多
Excel-based data analysis template for use with QuantiMIZE array and assay kits for Rotor-Disc 100 rings
显示更多
Excel-based data analysis template for use with QuantiMIZE array and assay kits for 384-well plates
显示更多
fragment fix placeholder