从血浆,血清或无细胞体液中同时纯化病毒DNA和RNA
QIAamp MinElute Virus Vacuum Kit使用快速真空操作简化纯化病毒DNA和RNA的操作流程。QIAamp MinElute Virus Vacuum Kit起始样品体积可多至0.5 ml。该试剂盒同时具备硅胶膜选择性结合DNA的特性,以及20–150 µl的灵活洗脱体积。
纯化的病毒核酸不含蛋白质、核酸酶或其他杂质,适用于PCR和RT-PCR等多种敏感的下游应用(参见"High sensitivity in PCR"和"High sensitivity in RT-PCR")。
使用QIAamp MinElute Virus Vacuum Kit纯化的病毒核酸适用于多种下游应用,包括:
QIAamp MinElute Virus Vacuum Kit使用快速离心操作,简化了从血浆、血清或无细胞体液中分离病毒RNA和DNA的操作流程,无需苯酚-氯仿抽提。核酸特异性结合到QIAamp MinElute硅胶膜上,污染物流出。可通过两步高效洗涤完全去除二价阳离子或蛋白质等PCR抑制剂,再用水或试剂盒中的缓冲液洗脱结合在离心柱上的纯核酸。QIAamp MinElute技术从血浆、血清或无细胞体液中纯化的病毒RNA和DNA,可即时用于PCR和印迹实验中。
QIAamp MinElute Virus Vacumm Kit同时具备硅胶膜选择结合DNA的特性,以及20–150 µl的灵活洗脱体积。QIAamp样本制备技术完全得到认可验证。
QIAamp MinElute Virus Vacuum Kit使用成熟技术可同时纯化病毒RNA和DNA。对新鲜或冷冻的血浆、血清或其他无细胞体液(500 µl)的处理在1小时内即可完成。
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The percentage of positive samples at low virus titers is shown. Nucleic acids from a DNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subjected to PCR. For the spin procedure, 95% probit values for 40 samples were 27.25 IU/ml and for the vacuum procedure, they were 9.30 IU/ml.
The percentage of positive samples at low virus titers is shown. Nucleic acids from an RNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subject to RT-PCR. For the spin procedure, 95% probit values for 32 samples were 22.84 IU/ml and for the vacuum procedure, they were 9.46 IU/ml.