GeneRead DNAseq Custom Panel V2
For targeted enrichment of a customized set of genes or genomic regions specific for your NGS needs
GeneRead DNAseq Custom Panels V2 are the simplest tools for analyzing the genetic variants of a customized panel of genes or genomic regions via next-generation sequencing. Each panel consists of multiplex PCR primer sets, sufficient for 480 samples, to amplify genomic regions of interest tailored to your specific NGS needs. Our primer design and targeted enrichment chemistry provide high design coverage, specificity, and uniformity, which are essential for detecting low-frequency variants in your precious samples.
Performance
The performance of GeneRead DNAseq Custom Panels V2 is assessed by three criteria: design coverage, specificity, and coverage uniformity. Greater than 90% of exonic regions are covered by the panel primer design, enhancing potential variant discovery. The high specificity of the system maximizes efficient use of sequencing capacity, as more than 90% of sequencing reads align to target regions. Finally, the high coverage uniformity of the system, with more than 90% of targeted bases covered at >20% median sequencing depth, ensures high-quality variant calls.
Principle
The GeneRead DNAseq Custom Panels V2 employ overlapping primer sets across the exonic portions of a gene or group of genes to maximize target coverage (see figure Multiplex PCR-enabled target enrichment of genomic regions of interest). Overlapping primer sets are divided into 4 pools, thereby increasing the specificity of coverage without primer dimers and nonspecific amplifications. Following amplification and purification, enriched regions from each sample are pooled together, yielding one library preparation for each sample.
Virtual panels are custom panels created from gene lists compiled by bioinformatics experts for specific diseases not covered by the GeneRead DNAseq Targeted Panels V2 catalog. For a complete list, scroll down to Applications.
Procedure
GeneRead DNAseq Custom Panels V2 are part of a total workflow for targeted next-generation sequencing (see GeneRead DNAseq Targeted Panels V2 workflow). Simply extract DNA from your samples (the QIAamp DNA Mini Kit, QIAamp DNA FFPE Tissue Kit, or the GeneRead DNA FFPE Kit are recommended), quantify and qualify your DNA sample with the GeneRead DNA QuantiMIZE System, and then use the GeneRead DNAseq Custom Panels V2 in combination with GeneRead DNAseq Panel PCR Kit V2 to perform targeted enrichment using multiplex PCR. Once targets have been enriched, construct the NGS library with the GeneRead Library Prep Kits and use the GeneRead DNAseq Library Quant Array to quantify and perform quality control. Perform NGS and analyze your data using the QIAGEN NGS Data Analysis Web Portal.
Applications
GeneRead DNAseq Custom Panels V2 are highly suited for enriching a panel of any genomic regions of interest in the human genome to determine genetic variants through NGS analysis. Virtual panels are highly suited for enriching a panel of recommended genomic regions of interest for particular diseases and pathways.
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