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PAXgene Bone Marrow RNA Tubes

用于收集骨髓样本,并稳定/纯化细胞内RNA

  • 完整的体系,用于采集、稳定和纯化
  • 收集时立即稳定胞内RNA
  • 从骨髓中快速纯化RNA
  • 安全方便的运输

PAXgene Bone Marrow RNA System包含PAXgene Bone Marrow RNA Tube收集管和PAXgene Bone Marrow RNA Kit纯化试剂盒,前者用于稳定、储存和运输骨髓样本,后者应用硅胶膜和离心柱技术对RNA进行分离纯化。骨髓样本可以用标准的医学方法收集并加入常用的抗凝剂。每份2 ml的骨髓样本立即存入一支PAXgene Bone Marrow RNA Tube中,该收集管可以立即稳定样本中的RNA,并适合在室温、冷藏或冷冻条件下保存。样本可在几天后使用PAXgene Bone Marrow RNA Kit进行纯化,也可在–20°C或–70°C条件下保存数月。

Cat No./ID: 764114
PAXgene Bone Marrow RNA Tubes (50)
50 Bone Marrow Collection Tubes. To be used in conjunction with the PAXgene Bone Marrow RNA Kit

仅限于科研应用,不适用于诊断,不为疾病的诊断、预防或治疗提供参考信息。


0
Stability of 5 transcripts at room temperature.
The changes in mRNA levels (ΔCT) from day 0 to day 2 for the samples in figure "RNA stability at room temperature" were calculated for each transcript quantified. The minimal changes and small standard deviation for samples stabilized in PAXgene Bone Marrow RNA Tubes contrasts with the significant changes for samples stored in EDTA tubes.
1
RNA yields from bone marrow samples.
Bone marrow samples were stabilized in PAXgene Bone Marrow RNA Tubes, and RNA was purified using the PAXgene Bone Marrow RNA Kit. Yields are indicated for 295 samples, from both in-house and external studies. The median yield for the 295 samples was 11.0 μg RNA per 2 ml sample. Note that RNA yields can vary greatly due to the extreme heterogeneity of bone marrow samples.
2
RNA stabilized at 2–8°C.

Bone marrow samples were collected in duplicate and stabilized in PAXgene Bone Marrow RNA Tubes. RNA was purified from the samples using the PAXgene Bone Marrow RNA Kit with no storage or after 5 days storage at 2–8°C. RNA integrity was evaluated using the RNA integrity numbers (RIN), measured on the Agilent 2100 Bioanalyzer. Results are shown for 3 representative donors. The RIN did not change significantly during storage, indicating that the RNA remains stable in the sample.

3
High RNA integrity after freeze–thaw cycles.

Bone marrow samples were stabilized in PAXgene Bone Marrow RNA Tubes, and RNA was purified using the PAXgene Bone Marrow RNA Kit after the indicated number of freeze–thaw cycles. The RIN values did not change significantly, indicating that RNA remained intact in the samples up to 2 freeze–thaw cycles.

4
RNA stability at room temperature.
Bone marrow samples were collected from 39 individuals and immediately transferred to [A] PAXgene Bone Marrow RNA Tubes or [B] EDTA tubes. RNA was purified using the PAXgene Bone Marrow RNA Kit or a reference silica-membrane-based method at day 0 or after 2 days storage at room temperature. Expression levels of 5 marker transcripts for each sample (IL-8, p53, cox-2, IL-1β, c-fos) were quantified using real-time RT-PCR. The high correlation of CT values (threshold cycles) for the samples stabilized in PAXgene Bone Marrow RNA Tubes indicates that the levels of these transcripts did not significantly change during storage, in contrast to the samples stored in EDTA tubes.
5
Storage under different conditions.
Bone marrow samples were stored in PAXgene Bone Marrow RNA Tubes [A] for 3 days at 18–25°C, [B] for 5 days at 2–8°C, or [C] subjected to 2 freeze–thaw cycles. RNA was purified using the PAXgene Bone Marrow RNA Kit, and RNA purity was analyzed by absorbance. The A260/A280 ratios indicate highly pure RNA from samples stored under different conditions.
Performance
PAXgene Bone Marrow RNA Kit可从不同的人类骨髓样本中高效分离和纯化细胞内RNA(参见"RNA yields from bone marrow samples"),包括多细胞、黏性样本的优化实验方案。 样品可在室温、冷藏或冷冻条件下保存几天后使用PAXgene Bone Marrow RNA Kit进行纯化,获得完整的高品质RNA(参见"RNA stability at room temperature"、"RNA stabilized at 2–8°C"和"High RNA integrity after freeze–thaw cycles")。RNA Tubes可防止RNA分子被RNases降解,并减少体外基因表达的变化,确保准确进行分析(参见"Stability of 5 transcripts at room temperature")。试剂盒自带DNase 处理,确保基因组DNA残留量达到最小。内部和外部研究均表明,95% 的检测样本(n=157)中的基因组DNA含量小于0.85% 。A260/A280值一般在1.8–2.2之间,表明产物为高品质RNA(参见"Storage under different conditions")。
Principle

在室温下储存或运输骨髓样本时,骨髓中每种mRNA的拷贝数会有巨大变化,给基因表达研究带来了巨大挑战。PAXgene Bone Marrow RNA Tubes可防止RNA分子被RNases降解,并适合于在室温、冷藏或冷冻条件下保存。

Procedure

骨髓样本可以用标准的医学方法收集并加入常用的抗凝剂。每份2 ml的骨髓样本立即存入一支PAXgene Bone Marrow RNA Tube中,该收集管可以立即稳定样本中的RNA ,并适合于在室温、冷藏或冷冻条件下保存。PAXgene Bone Marrow RNA Kit可方便、快速的从人类骨髓样本中分离并纯化细胞内RNA 。该试剂盒使用离心柱,采用硅胶膜技术,可高效纯化完整的高品质RNA 。

Applications

使用PAXgene Bone Marrow RNA Kit纯化的RNA可即用于各种下游应用,包括:

Real-time RT-PCR分析特定的转录产物
复杂的表达谱芯片分析
Features
Specifications
Additive/concentration Additive 11.1 ml
Closure type/color BD Hemogard/Red
Draw volume 2 ml
Label type Paper
Quantity 50/box
Tube size 16 x 125 mm

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产品介绍与指南 (3)

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For collection, storage, and transport of bone marrow samples and stabilization and purification of intracellular RNA

 


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试剂盒操作手册 (2)
For isolation and purification of intracellular RNA from whole bone
marrow samples stabilized in PAXgene Bone Marrow RNA Tubes
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For the collection of human bone marrow and immediate stabilization of intracellular RNA
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