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Qproteome O-Glycan Glycoprotein Kit

用于富集T-抗原型糖基化蛋白
  • 基于糖蛋白糖基结构的特性对糖蛋白进行高特异性的分离
  • 可分析不同条件或不同疾病状态下的细胞中的糖蛋白
  • 选择凝集素柱子精确分析糖蛋白
The Qproteome O-Glycan Glycoprotein Kit contains two different lectin-resin-filled spin columns, buffers, and reagents for the isolation of glycoproteins with a glycan structure of the type that are found on T-antigens from 6 cell lysate or serum samples. The kit can be automated on the QIAcube.

Cat No./ID: 37571
Qproteome O-Glycan Glycoprotein Kit
For 6 O-glycan glycoprotein preps: AIL and PNA Lectin Spin Columns (3 each); Buffers; Protease Inhibitor Solution; Collection Tubes (6 x 2 ml)

Qproteome O-Glycan Glycoprotein Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


Performance
The Qproteome O-Glycan Glycoprotein Kit enables highly specific enrichment of glycoproteins with a glycan structure of the type that are found on T-antigens (see figure Highly specific glycoprotein fractionation). Glycoprotein profiling of cells grown under different conditions or in different disease states can be carried out reliably. The selection of lectin columns allow precise glycoprotein characterization.
Principle
Glycosylated proteins with a glycan structure of the type that are found on T-antigens bind to lectins immobilized on the resin in the spin columns. After washing, proteins are eluted by addition of an elution buffer containing sugars which compete for lectin binding sites with the bound proteins.
Procedure
The sample is diluted and applied to the spin column (see figure Glycoprotein fractionation procedure). After a short incubation, proteins that have not bound to the lectin are removed by centrifugation. Bound proteins are eluted by application of elution buffer and centrifugation.
Applications
Glycosylation plays a vital role in a wide range of cellular processes such as cell adhesion and signaling, stabilization of protein structure and function, protein trafficking and sorting, and oncogenesis. Several diseases (e.g., rheumatoid arthritis, muscular dystrophy) may be caused by a defect in protein glycosylation. Qproteome Glycoprotein Fractionation Kits offer highly specific separation of glycoproteins according to the structure of their glycan moieties, and permit profiling of glycoproteins in cells grown under different conditions.
Features
Specifications
应用 SDS-PAGE, mass spectrometry
结合能力/产量 30–150 µg
糖蛋白:哪种类型糖蛋白 Glycoproteins with a glycan structure
分离组分 One fraction
翻译后修饰蛋白 Glycosylation
样本大小 50 µl
起始材料 Serum