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QIAamp MinElute ccfDNA Kits

For isolation of free-circulating DNA from human plasma or serum
  • Efficient recovery of fragmented DNA
  • High yield ensuring detection of low frequency biomarkers
  • Low elution volume in water (20 µl) for high concentration of nucleic acids
  • Scalable sample input volume of 1–10 ml
  • Removal of contaminants and inhibitors
QIAamp MinElute ccfDNA Kits greatly simplify concentration and purification of free-circulating DNA from plasma or serum. The procedure can be partially automated on the QIAcube.
Cat No./ID: 36912
12-Tube Magnet
Magnet for separating magnetic beads in 12 x 1.5 ml or 2 ml tubes
Cat No./ID: 36935
15 ml/50 ml Tube Magnet 
Magnet for separating magnetic particles in 5 x 15 ml and 3 x 50 ml tubes
Cat No./ID: 55204
QIAamp MinElute ccfDNA Mini Kit (50)
For 50 preps (1 or 2 ml sample input volume each): QIAamp UCP MinElute Columns, QIAGEN Proteinase K, Magnetic Bead Suspension, Buffers, Bead Elution Tubes, Collection Tubes (1.5 ml and 2 ml)
Cat No./ID: 55284
QIAamp MinElute ccfDNA Midi Kit (50)
For 50 preps (4 or 5 ml sample input volume each): QIAamp UCP MinElute Columns, QIAGEN Proteinase K, Magnetic Bead Suspension, Buffers, Bead Elution Tubes, and Collection Tubes (1.5 ml and 2 ml)
Cat No./ID: 768115
PAXgene Blood ccfDNA Tubes (100)
100 blood collection tubes (10 ml). To be used in conjunction with the QIAamp MinElute ccfDNA Kit, the QIAamp Circulating Nucleic Acid Kit (50) or the QIAsymphony PAXgene Blood ccfDNA Kit (192)
Cat No./ID: T-1-700
AdnaMag-L
For 8 tubes, 15 ml
Cat No./ID: T-1-800
AdnaMag-S
For 8 tubes, 1.5 ml

QIAamp MinElute ccfDNA Kits适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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Faster access to ccfDNA
Comparison of sample processing time (for 4 samples of 4 ml plasma each, using commercially available kits) shows that the QIAamp MinElute ccfDNA Kit provided the fastest processing.
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Highest yield of ccfDNA
Total yield of a 66 bp ccfDNA fragment from 4 ml of plasma is shown using the QIAamp MinElute ccfDNA Kit compared with 5 other commercially available kits. Results show that the QIAamp MinElute ccfDNA Kit provided higher DNA yield than competitor solutions.
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Highest ccfDNA concentration
ccfDNA concentrations of a 66 bp fragment were compared, using 5 commercially available kits and the QIAamp MinElute ccfDNA Kit. The QIAamp MinElute ccfDNA Kit provided the highest DNA concentration in the final eluate.
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Flexible input volume
Total yield of a 66 bp ccfDNA fragment is shown with sample input volumes from 1 to 8 ml, using QIAamp MinElute ccfDNA Kits.
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QIAamp MinElute ccfDNA Kit procedure
Performance
The quantity of free-circulating DNA in plasma, serum and other body fluids is usually low, and isolation is a challenge. QIAamp MinElute ccfDNA Kits provide excellent results with efficient purification of high-quality ccfDNA from serum and plasma samples, even when small amounts are present. Compared with other commercially available kits, QIAamp MinElute ccfDNA Kits recovered the greatest amount of ccfDNA.
Pre-concentration of ccfDNA on magnetic beads allows flexible and scalable handling of volumes from 1 to 10 ml while the use of a MinElute column allows flexible elution volumes between 20 μl and 80 μl, providing the highest possible concentration of nucleic acid species that are present in low concentrations (typically 1–100 ng/ml circulating DNA in human plasma). ​
The QIAamp MinElute ccfDNA Mini Kit has been optimized for sample volumes from 1 to 4 ml while the QIAamp MinElute ccfDNA Midi Kit has been optimized for sample volumes from 4 to 10 ml. QIAamp MinElute columns can bind fragmented nucleic acids that are as short as 20 bases, but yield depends on the sample volume and the concentration of circulating nucleic acids in the sample.​
Principle
Free-circulating DNA, such as tumor-specific extracellular DNA fragments in the blood or fetal DNA in maternal blood, is present in serum or plasma usually as short fragments of <1000 bp. Because ccfDNA may reflect the entire tumor genome, it has gained attention for its potential clinical utility, enabling use of “liquid biopsies” in the form of blood draws.
QIAamp MinElute ccfDNA Kits enable efficient purification of these circulating DNAs from human plasma or serum. ​The procedure can be partially automated on the QIAcube.
Procedure
The kits use a fast procedure involving pre-concentration of circulating nucleic acids onto magnetic beads and a cleanup of the resulting pre-eluate on QIAamp MinElute columns in a quick and convenient spin procedure. The robust procedure helps to eliminate sample-to-sample cross-contamination and increases user safety when handling potentially infectious samples.
The simple procedure, which is highly suited for simultaneous processing of multiple samples, provides pure nucleic acids in less than 1 hour for 24 samples. Free-circulating DNA is eluted in Ultra-clean Water, ready for use in downstream reactions or storage a –30 to –15°C. Purified nucleic acids are free of proteins, nucleases and other impurities. The procedure can be partially automated on the QIAcube.
Applications
QIAamp MinElute ccfDNA Kits enable efficient purification of circulating DNAs from human plasma or serum.

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试剂盒操作手册 (3)

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For collection of whole blood and stabilization of ccfDNA
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for separation of BioMag® particles in 15 ml or 50 ml tubes
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常见问题 (34)
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