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Gentra Puregene Yeast/Bact. Kit

从酵母和革兰氏阳性菌或阴性菌中纯化高品质DNA

  • 高品质DNA用于长期存档
  • 可重复的DNA纯化
  • 从样本到储存的完整纯化解决方案
  • 方便、可扩展的纯化过程
Gentra Puregene Yeast/Bact. Kit纯化高分子量DNA(100–200 kb),适合用于存档。可扩展的纯化操作,能温和的去除样本中的污染物和抑制剂,纯化获得的DNA适合长期保存。
Cat No./ID: 158567
Gentra Puregene Yeast/Bact. Kit (200 ml)
For purification of archive-quality DNA from 200 ml bacterial cultures or 400 ml yeast cultures: Cell Suspension Solution, Lytic Enzyme Solution, RNase A Solution, and Reagents

Gentra Puregene Yeast/Bact. Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


1
纯化获得存档质量DNA,具有可重复性。
使用Gentra Puregene Yeast/Bact. Kit从酵母培养样本中纯化DNA。纯化的DNA(100 ng)进行琼脂糖凝胶电泳分析。M1: Uncut Lambda DNA;M2: Lambda HindIII标记物。
Performance
可存档的DNA

DNA的纯度对下游应用结果的准确性至关重要,诸如PCR等敏感的下游应用要求使用最高品质、且分子量尽可能大的DNA,以确保可靠的结果。Gentra Puregene Yeast/Bact. Kit去除污染物和酶抑制剂,确保获得高度稳定的非常适合存档的DNA。纯化获得的高品质DNA分子大小为100–200 kb(参见"Reproducible purification of archive-quality DNA")。

Principle
细胞用阴离子洗涤剂裂解,其中加入DNA稳定剂。DNA稳定剂限制胞内DNase的活性,DNA稳定剂也存在于其他环境中。使用RNA降解酶去除RNA。蛋白质等其他污染物通过盐析沉淀法去除。最终,通过乙醇沉淀回收基因组DNA,用水合溶液(1 mM EDTA 、10 mM Tris·Cl pH 7.5))溶解DNA。一般情况下,纯化的DNA的A260/A280值在1.7–1.9之间,大小可达200 kb。纯化的DNA可贮存于2–8°C、–20°C 或–80°C。
Procedure
简单的Puregene流程应用经修饰的盐析沉淀法纯化DNA(参见"Puregene DNA procedure")。无需混合或稀释溶液,并且确保手工操作时间最小化。流程提供方便的实验终止点,可暂时保存部分纯化的样品,安全可靠。
Applications

Gentra Puregene Yeast/Bact. Kit纯化得到的DNA非常稳定,适合于多种下游应用,包括:

  • DNA存档
  • PCR
  • SNP分析
  • Southern印迹
Features
Specifications
应用 PCR, restriction digest, Southern analysis, SNP analysis
洗脱体积 50 µl (varies)
规格 Scalable
主要样本类型 Gram+ and Gram- Bacteria, Yeast
处理 Manual
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 Bacterial DNA / Yeast DNA
样本量 0.5-1.5*10e9 cells / 1-2*10e8 cells
技术 Modified salting-out precipitation method
每次运行或制备时间 25 - 60 minutes (+ DNA rehydration)
产量 17-50 µg/ml culture / 4.5 µg/ml culture

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试剂盒操作手册 (1)
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补充实验方案 (4)
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