text.skipToContent text.skipToNavigation

QIAcube HT

96孔板形式的中高通量核酸自动纯化仪
  • 可从几乎任何类型样本中纯化质量可靠的DNA、RNA和miRNA
  • 采用专用纯化试剂盒的高通量解决方案,节省成本和时间
  • 用户友好的软件,可以方便地进行数据管理和存档
  • 创新的设计在提高安全性的同时最大限度控制交叉污染风险
  • 仪器方便、灵活、易用,占用面积小
QIAcube HT采用硅胶膜技术,实现96孔板形式的中高通量自动核酸纯化。用户可从几乎任何类型的样本(包括细胞、组织、食物、以及动物样品中的细菌和病毒)中轻松、快捷地纯化DNA、RNA和miRNA。自动化实验方案和专用的QIAcube HT纯化试剂盒和耗材,增加了实验的可靠性和便利性,同时节省了宝贵的时间。现有QIAxtractor的用户可以轻松地升级自己的系统,享受到这些新功能和新特性。升级需要安装附件包(Accessories Pack),QXT和QIAcube HT软件。更多详情请参阅QIAcube HT升级页面。
Cat No./ID: 9001793
QIAcube HT System
Robotic workstation with UV lamp, HEPA filter, laptop, QIAcube HT operating software, startup pack, installation and training, 1-year warranty on parts and labor
Cat No./ID: 9001899
QIAcube HT Priority Package
Robotic workstation with UV lamp, HEPA filter, laptop, QIAcube HT operating software, startup pack; includes Priority Package with installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
Cat No./ID: 9001900
QIAcube HT Priority Package Plus
Robotic workstation with UV lamp, HEPA filter, laptop, QIAcube HT operating software, startup pack; includes Priority Package Plus with installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
Cat No./ID: 9022649
Accessories Pack, QXT
For upgrading QIAxtractor instruments to process dedicated QIAcube HT kits: Transfer Carriage, Riser Block EMTR, and Channeling Adapter
QIAcube HT适用于分子生物学应用。该产品不可用于疾病的诊断、预防或治疗。
0
Analysis of genomic DNA purified from human blood.
Human blood from one donor was treated with sodium citrate, heparin, or EDTA. Genomic DNA was purified from a 50 or 200 µl aliquot of the treated blood manually with the QIAamp 96 DNA Blood Kit or on the QIAcube HT using the QIAamp 96 DNA QIAcube HT Kit with the QIAamp 96 DNA QIAcube HT V1.QSP protocol. Purified DNA was eluted in 200 µl. PCR amplification of a 1.2 kb fragment from the single copy Hugl gene was carried out using QIAGEN HotStarTaq DNA Polymerase and 2.5 µl eluate in a total volume of 25 µl. A 1 µl aliquot of each PCR was analyzed on the QIAxcel Advanced with the QIAxcel DNA Screening Kit. M1: pUC18 HaeIII marker; M2: phiX174 HaeIII marker.
1
从组织中自动纯化高品质RNA。
RNA分离自通过RNAlater RNA Stabilization Reagent稳定的10 mg大鼠组织(肺、肾、心脏、脑、脾和肠)。在QIAcube HT和BioRobot Universal System,或使用QIAzol Lysis Reagent的QIAcube全自动核酸纯化仪上自动处理样品。取1 µl每个纯化的RNA样品在QIAxcel Advanced上用QIAxcel RNA QC Kit v2.0和QX RNA Size Marker 200–6000 nt进行分析。由此得到较高的RNA完整性得分(RIS),即表明回收到高品质的RNA。
2
Reliable automated purification.
Viral nucleic acids were purified from 200 µl plasma samples spiked with 10,000, 1000 and 100 IU/ml of a typical DNA or RNA virus. Sample processing was automated using either QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol, QIAxtractor with the VX Protocol, or QIAcube with the QIAamp MinElute Virus Spin Kit. Viral nucleic acids were detected using in-house PCR and RT-PCR assays, with 20 µl eluate per reaction on the Rotor-Gene Q. Results show that QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit performs as well as or better than the other methods.
3
Reliable purification from a range of sample types.
Various sample types were spiked with 20,000 IU of a typical DNA virus. Viral DNA was purified from 200 µl of each sample lysate using the QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol. Purified viral DNA was detected using an in-house PCR assay with 20 µl each eluate per reaction.
4
Efficient PCR inhibitor removal with all kits whether used manually or automated.
Aliquots of 200 mg of three different human stool specimens were processed on the QIAcube HT using the QIAamp 96 PowerFecal QIAcube HT Kit, on the QIAcube using the QIAamp DNA Stool Mini Kit and QIAamp Fast DNA Stool Mini Kit, or manually using the two DNA Stool kits. Real-time PCR was carried out on the Rotor-Gene Q using the QuantiTect Virus PCR Kit with 2 µl and 10 µl eluates respectively, from each sample in 20 µl reactions containing an internal control. CT values were compared to a standard containing no sample but only internal control (dark blue bar). All the three samples either extracted on different instruments or manually showed similar performance compared to the standard, regardless of the volume of eluate used, indicating no PCR inhibition.
5
Automated bacterial DNA purification from various animal stool samples gave comparable yields to the manual procedure.
Aliquots of 200 mg stool sample from different animals were processed on the QIAcube HT instrument (blue bars) using the QIAamp 96 PowerFecal QIAcube HT Kit, or manually using the QIAamp Fast DNA Stool Mini Kit (pink bars). Real-time PCR of 16S rRNA gene of Corynebacterium glutamicum was carried out on the Rotor-Gene Q using the QuantiFast Probe PCR Kit with 1 µl eluate in a 20 µl reaction. DNA extracted either manually or on the QIAcube HT instrument showed similar performance for all the stool samples.
6
Co-isolation of viral RNA and bacterial DNA.
Consistent amounts of BVDV particles and Salmonella cells were simultaneously spiked into a variety of animal specimens. Samples were processed in parallel on the QIAcube HT with the cador Pathogen 96 QIAcube HT Kit or manually with the QIAamp cador Pathogen Mini Kit. Co-isolated viral RNA and bacterial DNA were identified using an in-house assay. Mean CT values are shown for [A] BVDV and [B] Salmonella. Small error bars (±1 SD of 3 extraction replicates) indicate high precision for both purification procedures.
7
高效快速的自动DNA抽提。
针对在QIAcube HT仪器上从食品原材料及加工过的食品材料中进行中高通量快速DNA纯化的需要,DNeasy mericon 96 QIAcube HT Kit进行了改进。与传统CTAB实验方案相比,改进的十六烷基三甲基溴化铵(CTAB)提取流程大幅缩短了从食物样本中提取DNA的时间。
8
Highly repeatable viral RNA isolation.
Blood samples of various species were spiked with BVDV particles and were frozen at –20°C. Of each sample, 4 replicates were processed on QIAcube HT with the cador Pathogen 96 QIAcube HT Kit in 3 subsequent runs on different days. [A] Viral RNA was identified using an in-house assay, which included [B] an internal amplification control. Error bars represent ±1 SD of 3 replicates. The BVDV CT values for each set of quadruplicates showed a mean relative standard deviation of 0.67 ± 0.43%, and the inter-assay CV for each sample from 3 runs ranged from 0.14 to 0.89% (mean: 0.45%), indicating high repeatability of viral RNA purification on QIAcube HT.
9
High performance in downstream assays.
Genomic DNA was purified from 200 µl human blood. Samples were processed with the QIAcube HT using the QIAamp 96 DNA QIAcube HT Kit and the QIAamp 96 DNA QIAcube HT V1.QSP protocol, or with the QIAcube using the QIAamp DNA Blood Mini Kit. Quantitative PCR of IL8 was carried out using the QuantiFast Probe Kit with 5 µL eluate in 25 µl. The CT values obtained show even amplification across the whole 96-well plate, indicating no well-to-well variation in DNA recovery with QIAcube HT. CT values were similar between the different purification platforms.
10
High sensitivity of purified viral nucleic acids in PCR and RT-PCR.
Nucleic acids were purified from serial dilutions of plasma samples spiked with a typical RNA or DNA virus. Samples were processed using QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol and were analyzed using in-house PCR and RT-PCR assays. The percentage of positive samples at low virus titers is shown. The resulting 95% probit values were 316.84 IU/ml for the RNA virus and 18.54 IU/ml for the DNA virus.
11
从组织裂解液中回收的总RNA符合线性规律。
从通过RNAlater稳定的大鼠肺组织中提取裂解液,然后梯度稀释以获得等同于2.5–25 mg组织的样品。通过RNeasy QIAcube HT Kit和RNeasy 96 QIAcube HT total RNA Tissue V1.QSP实验方案,在QIAcube HT上从这些样品中纯化总RNA。用100 μl无RNase水洗脱纯化所得的RNA。光密度测定结果显示,RNA回收率和起始材料量之间为线性关系。
12
Purification of DNA from complex food samples.
DNA was purified from orange juice, chocolate, and peanut butter using the QIAcube HT instrument and the DNeasy mericon 96 QIAcube HT Kit or manually using the DNeasy mericon Food Kit. Purified DNA was analyzed by qPCR of the trnL chloroplast tRNA gene using the QuantiFast SYBR® Green PCR Kit. The resulting CT values indicate similar performance from both purification methods. Diluting the input DNA 1:10 for qPCR analysis shows that all purified DNA was also free of PCR inhibitors.
13
Purification of genomic DNA from tissues.
Genomic DNA was purified from 5 mg rat lung, kidney, spleen, and liver tissue and 200 µl human blood. Samples were processed with the QIAcube HT using the QIAamp 96 DNA QIAcube HT Kit and the QIAamp 96 DNA QIAcube HT V1 QSP protocol or with the QIAcube using the QIAamp DNA Blood Mini Kit (blood) or the QIAamp DNA Mini Kit (tissues). Both platforms showed similar performance in [A] yield and [B] quality of purified DNA for each type of starting material.
14
RNA纯化具有可高度可重复性。
通过RNeasy 96 QIAcube HT Kit和RNeasy 96 QIAcube HT total RNA cell with DNase V1.QSP实验方案,在QIAcube HT上从5×105个Jurka细胞中纯化RNA。100 μl无RNase水洗脱纯化所得的RNA。使用QuantiFast Probe RT-PCR Kit以5 µl洗脱液为模板,25 µl定量PCR反应体系,进行人源β-肌动蛋白mRNA定量RT-PCR反应。单个96孔板的反应所得的CT值显示,整个平板的扩增量基本相等,从而表明RNA回收不存在孔间差异。
Performance
QIAcube HT提供快速、可靠的自动化高通量核酸纯化,产物适用于各种敏感的下游应用。研究人员可以便利地提高通量,纯化产物和从其他QIAGEN核酸纯化平台获得的核酸一样拥有高品质。

DNA纯化
通过QIAcube HT和专用的QIAamp 96 DNA QIAcube HT Kit,可从血液、细胞、组织样本中轻松、快捷、可靠地自动纯化总DNA。此系统可处理含有常用抗凝剂的新鲜或冷冻全血(参见Analysis of genomic DNA purified from human blood)。QIAcube HT允许用户提高样品纯化通量而不影响质量和可靠性,产物DNA纯度高,在下游分析中具有良好表现(参见Purification of genomic DNA from tissues和High performance in downstream assays)。

RNA纯化
通过QIAcube HT纯化和RNeasy 96 QIAcube HT Kit,可简便、可靠地纯化RNA,并可确保96孔板的孔间无显著差异的线性样本回收率(参见Linear recovery of total RNA from tissuesReproducible 96-well RNA purification)。这种经济、易用的系统具有与其他值得信赖的QIAGEN纯化技术相同的出色表现(参见Automated purification of high-quality RNA from tissues)。

从动物样品中纯化DNA和RNA
QIAcube HT能在最大限度控制硅胶膜堵塞风险的前提下,从未经稀释的动物全血中可靠地纯化核酸。自动纯化均以96孔板的形式进行,具有准确、可重复的特点,不同时间、不同批次的纯化过程具有可靠表现,为您带来便利、安心(参见Highly repeatable viral RNA isolation)。通过QIAcube HT和专用cador Pathogen QIAcube HT Kit,可以自动共纯化病毒RNA和细菌DNA,且可获得与采用QIAamp cador Pathogen Mini Kit手动纯化同等的高质量核酸(参见Co-isolation of viral RNA and bacterial DNA)。

从复杂食物样本中纯化DNA
针对在QIAcube HT仪器上从食物原材料及加工过的食品材料中高通量快速纯化DNA的需要,DNeasy mericon 96 QIAcube HT Kit专门进行了改进。与传统CTAB实验方案相比,改进后的十六烷基三甲基溴化铵(CTAB)提取流程大幅缩短了从食物样本中提取DNA的时间(参见Efficient and rapid DNA extraction from complex food samples)。现在用户可以通过快速、简单的自动流程,制备出与采用DNeasy mericon Food Kit手动提取同等高品质的DNA(参见Purification of DNA from complex food samples)。
Principle
采用QIAcube HT进行自动纯化快速、经济,每次运行可从24–96个样本中纯化得到高品质核酸。专用QIAcube HT Kit适合从不同类型的起始原料中纯化核酸,包括细胞、组织、细菌、病毒和食品材料。QIAcube HT非常易用,其直观的软件可以让用户只需点击几下鼠标就可开始运行。

目前已有四种与QIAcube HT配套的专用纯化试剂盒上市。QIAamp 96 DNA QIAcube HT Kit用于从血液、细胞和组织等样本中自动纯化总DNA。RNeasy 96 QIAcube HT Kit可从动物和人体细胞组织等样本中提取总RNA或包括miRNA的总RNA;cador Pathogen 96 DNA QIAcube HT Kit适于从动物样本中自动纯化病毒RNA和DNA以及细菌DNA;DNeasy mericon 96 QIAcube HT Kit适于从很多食物原材料和已加工食品样品中自动纯化总核酸。样品纯化必需的所有耗材都包含在QIAcube HT Plasticware组件中,便于使用。配套提供的预设计实验方案和仪器运行文件可自QIAcube HT目录页上的“Resources”(资源)选项标签下下载,为用户在实验室快速安装并使用提供便利。

QIAcube HT是一台结构紧凑的台式仪器,具有非常小的占地面积,可轻松装配到大多数实验台,并且可直接移入生物安全实验室。为增加灵活性,QIAcube HT配备有笔记本电脑。

包括半透明罩在内的高级仪器安全功能有利于保护珍贵样品免受环境污染。HEPA过滤器可使罩下的工作台处于正压洁净空气中,保护珍贵样品免受空气污染影响。UV灯可对工作台进行高效净化处理,有助于防止交叉污染。QIAcube HT通过废吸头槽将用过的吸头弹出到仪器外侧的一次性塑料盒中。这种外部吸头弹出机制可防止在洁净的工作台上积聚废液。吸头回收盒支架可使回收盒保持在废吸头槽下方的正确位置上。该吸头回收盒还配有一个安全扣合盖,使废液处理简单、安全。
Procedure
QIAcube HT可自动、高通量纯化核酸,每次运行可处理24–96个样品(以8个样品为增量)。在样品纯化过程中,该仪器采用真空驱动,系统简单、快速地进行结合、洗涤和洗脱过程。真空室由两个单独的隔室组成。此配置是在纯化过程中最大限度地减少样品交叉污染的众多措施之一。

开始运行之前,将纯化样品需要的所有材料放置在仪器工作台上。在程序运行过程中,96孔样品板装载的起始材料被裂解并转移到过滤板。一次性样品槽包含样品结合、洗涤和洗脱所必须的所有缓冲液和试剂。移液吸头定位在工作台右侧。

液体转移任务由8通道移液头完成,此类移液头可在运行过程中快速转移液体。系统软件能监测吸头使用情况并在程序运行过程中实时更新吸头可用量。通过智能的吸头重复利用技术,QIAcube HT极大优化了耗材的使用。洗涤之后,移液头将96孔过滤板转移至洗脱位,在洗脱位,纯化所得的核酸被洗脱到洗脱微管。自带8联管盖可牢牢地扣在洗脱微管上以储存样品。
Software
QIAcube HT软件具有用户友好的QIAcube HT操作界面。其软件向导可模拟仪器工作台,您只需点击几下鼠标,即可轻松完成设置并开始运行。该软件可根据样品数量自动计算试剂量,并完整记录每次运行。该软件还允许您导入条码数据库、管理用户权限、整合实验室信息管理系统(LIMS)。
Features
Specifications
提供的附件 Vacuum Control Station, Vacuum Chamber, Pinch Valve, Channeling Adapter (Channeling Block and Channeling Block Holder), Transfer Carriage, Riser Block EMTR, Adapter Reagent Plate SBS, Adapter Reagent Plate R1, Tip Disposal Box Holder, Tip Chute, Tip Chute Protective Cap, Waste Bottle with Lid Assembly
交流 RS232 and USB
提供的耗材 QIAcube HT Plasticware (5 S-Blocks, 5 Elution Microtubes RS (EMTR), 120 x 8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad), 3x Tip Disposal Box with Lid, 2x Reagent Trough Lid 170 ml, 3x Reagent Trough with Lid 70 ml
尺寸 Instrument width: 540 mm (21 in.), height: 450 mm (18 in.), depth: 630 mm (25 in.); vacuum control station width: 495 mm (19 in.), height: 520 mm (20 in.), depth: 400 mm (16 in.)
电动 100–240 V AC, 250 VA, 50–60 Hz, overvoltage category II; mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages
HEPA过滤器 Preinstalled dual-cartridge 3M Air-Mate HEPA filter system provides positive pressure clean air within the closed hood.
湿度 Operating humidity 40-70%
提供笔记本电脑 QIAcube HT comes with a notebook computer for added flexibility.
移液体系 8 pipetting channels, pipetting volume (20-200 µl)
操作地点 For indoor use only
污染水平 2
精确度 20-200 µl, less than 4% coefficient of variation
软件 Supplied with unlimited user license with free upgrades by web download
表容量 4 x SBS positions (127.76 x 85.48 mm), compatible with 96-well plates or tip racks 1 custom adapter for a reagent trough plate compatible to accept reagent troughs vacuum chamber with chambers for waste and elution
通量 24-96 samples in increments of 8 samples
弃去吸头 All used tips ejected externally; no waste accumulation on the worktable
紫外灯 Preinstalled UV lamp for worktable decontamination
质保 1-3 years warranty, depending on service package
重量 QIAcube HT instrument: 41 kg (90 lb.); vacuum control station: 19 kg (42 lb.)

您无权限查看此资源

产品介绍与指南 (1)
For fast and reliable automated 96-well nucleic acid purification
显示更多
用户使用手册 (4)
Important note regarding new QIAcube HT declaration of conformity and instrument symbols
显示更多
Instrument user manual for automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology; for use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
显示更多

显示更多
Instrument user manual for automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology; for use with QIAcube HT Prep Manager Software.
显示更多
实验方案软件 (2)
Procedure for installing Q Protocols for QIAcube HT Kits on the QIAcube HT and the QIAxtractor. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
显示更多
These Q Protocols and installation files can only be used with QIAcube HT Operating software version 4.17 and are not compatible with the new QIAcube HT Prep Manager software.

QIAcube HT Prep Manager Software already includes all standard protocols. Additional protocols and kit configurations can be easily installed. Check for available updates under the "Operating Software" section of the "Product Resources" tab.
显示更多
操作软件 (3)
Files necessary for configuring the QIAcube HT Prep Manager Software to process QIAamp 96 PowerFecal QIAcube HT Kits. Not compatible with QIAcube HT Operating Software 4.17.

For kit configuration installation instructions, please refer to the QIAcube HT User Manual  chapter 4.4.5.
显示更多
QIAcube HT instrument operating software. We recommend installing the new improved QIAcube HT Prep Manager Software, which offers a state-of-the-art user-interface and a wizard-guided experimental setup for quick and easy purification experiments.

Important: It is not possible to install both QIAcube HT Operating Software 4.17 and QIAcube HT Prep Manager Software on the same computer. Please uninstall Prep Manager Software before installing QIAcube HT Operating Software 4.17. For more information, contact QIAGEN Technical Services.
显示更多
QIAcube HT instrument operating software. This software offers a state-of-the-art user interface and a wizard-guided experimental setup for quick and easy purification experiments.

For software installation instructions, please refer to the QIAcube HT Unpacking and Installation Instructions or to the QIAcube HT User Manual, chapter 4.4.1.

Important: It is not possible to install both QIAcube HT Prep Manager Software and QIAcube HT Operating Software 4.17 on the same computer. Please uninstall QIAcube HT Operating Software 4.17 before installing QIAcube HT Prep Manager Software. For more information, contact QIAGEN Technical Services.
显示更多
安全数据表 (1)
fragment fix placeholder