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Gentra Puregene Tissue Kit

从组织中纯化高品质DNA

  • 高品质DNA用于长期存档
  • 可重复的DNA纯化
  • 从样本到储存的完整纯化解决方案
  • 方便、可扩展的纯化操作
Gentra Puregene Tissue Kit纯化高分子量的DNA(100–200 kb),适合用于存档。可扩展的纯化操作,能温和的去除样本中的污染物和抑制剂,纯化获得的DNA适合长期保存。
Cat No./ID: 158667
Gentra Puregene Tissue Kit (4 g)
For 4 g tissue: RNase A Solution, Puregene Proteinase K, and Reagents
Cat No./ID: 158689
Gentra Puregene Tissue Kit (33 g)
For 33 g tissue: RNase A Solution, Puregene Proteinase K, and Reagents

Gentra Puregene Tissue Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


1
从组织中纯化高分子量DNA。
使用Gentra Puregene Tissue Kit从4个肌肉组织样本中纯化DNA。纯化的DNA(100 ng)进行琼脂糖凝胶电泳分析。M1: Uncut Lambda DNA;M2: Lambda HindIII标记物。
Performance
可存档的DNA 

DNA的纯度对下游应用结果的准确性至关重要,诸如PCR等敏感的下游应用要求使用最高品质、且分子量尽可能大的DNA,以确保可靠的结果。Gentra Puregene Tissue Kit去除污染物和酶抑制剂,确保获得高度稳定的非常适合存档的DNA。纯化获得的高品质DNA分子大小为100–200 kb(参见"High-molecular-weight DNA from tissue")。

Principle
细胞用阴离子洗涤剂裂解,其中加入DNA稳定剂。DNA稳定剂限制胞内DNase的活性,DNA稳定剂也存在于其他环境中。使用RNA降解酶去除RNA。蛋白质等其他污染物通过盐析沉淀法去除。最终,通过乙醇沉淀回收基因组DNA,用水合溶液(1 mM EDTA 、10 mM Tris·Cl pH 7.5)溶解DNA。一般情况下,纯化的DNA的A260/A280值在1.7–1.9之间,大小可达200 kb。纯化的DNA可贮存于2–8°C、–20°C 或–80°C。
Procedure
简单的Puregene流程应用经修饰的盐析沉淀法纯化DNA(参见"Puregene DNA procedure")。无需混合或稀释溶液,并且确保手工操作时间最小化。流程提供方便的实验终止点,可暂时保存部分纯化的样品,安全可靠。
Applications

Gentra Puregene Tissue Kit纯化得到的DNA非常稳定,适合于多种下游应用,包括:

  • DNA存档
  • PCR
  • SNP分析
  • Southern印迹
Features
Specifications
应用 PCR, restriction digest, Southern analysis, SNP analysis
洗脱体积 50-250 µl (varies)
规格 Scalable
主要样本类型 Tissue samples, fixed and paraffin-embeded tissue
处理 Manual
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 Genomic DNA
样本量 5-100 mg
技术 Modified salting-out precipitation method
每次运行或制备时间 25 - 60 minutes (+ cell lysis + DNA rehydration)
产量 Varies

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试剂盒操作手册 (1)
For purification of archive-quality DNA from human whole blood, bone marrow, buffy coat, buccal cells, body fluids, cultured cells, tissue, mouse tail, yeast, bacteria
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补充实验方案 (18)
This protocol is designed for purification of DNA from 50 μl or 1 ml samples of clotted whole blood using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit.
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This protocol is designed for purification of DNA from 2 ml samples of umbilical cord blood using the Gentra Puregene Tissue Kit or Gentra Puregene Blood Kit.
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This protocol is designed for purification of DNA from 50 μl samples of dried human blood using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit.
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This protocol provides information about scaling of reagents required for purification of DNA from 1 x 106 - 3 x 107 fixed cells using the Gentra Puregene Tissue Kit.
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This protocol provides information about scaling of reagents required for purification of DNA from 0.5-700 mg solid tissue using the Gentra Puregene Tissue Kit.
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This protocol provides information about scaling of reagents required for purification of DNA from 0.5-20 mg paraffin-embedded tissue using the Gentra Puregene Tissue Kit.
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This protocol is designed for purification of DNA from 1-3 ml samples of amniotic fluid containing 0.5-2 x 106 cells using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit.
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This protocol is designed for purification of DNA from 50 μl samples of CSF using the Gentra Puregene Tissue Kit.
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This protocol is designed for purification of DNA from 1-2 ml samples of Chlamydomonas using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit.
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This protocol is designed for purification of DNA from 5-10 mg or 10-20 mg samples of fungal tissue using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit.
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This protocol is designed for purification of DNA from a fecal swab or 1-2 g samples of fecal material using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit.
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This protocol is designed for purification of DNA from 10-20 mg samples of fish tissue using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit.
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This protocol is designed for purification of DNA from 5-10 mg or 10-20 mg samples of solid tissue fixed in ethanol or formalin using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit.
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This protocol is designed for purification of DNA from 5 hair roots using the Gentra Puregene Tissue Kit.
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This protocol is designed for purification of DNA from 0.1-0.3 mg samples of bone fragments using the Gentra Puregene Tissue Kit.
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This protocol is designed for purification of DNA from 5-20 mg samples of marine invertebrate tissue using the Gentra Puregene Tissue Kit.
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This protocol is designed for purification of DNA from 40-90 mg (50-75 μl), 80-180 mg (100-150 μl), or 20 g samples of nematodes or nematode suspensions using the Gentra Puregene Tissue Kit.
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This protocol is designed for purification of DNA from 1 ml samples of whole blood using the Gentra Puregene Tissue Kit.
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参考文献
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