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GeneRead DNAseq Library Quant Array

通过qPCR技术定量NGS文库
  • 提供梯度稀释的DNA标准品,无需手动滴定
  • 通过质量评分,在测序前去除质量较差的文库
  • Excel文件的数据分析可自动计算文库浓度
  • 实验方案简便,灵敏性高
  • 与常用的NGS仪器和qPCR仪兼容
GeneRead DNAseq Library Quant Array为即用型解决方案,用于在测序前,对GeneRead DNAseq Gene Panel文库进行定量和质量评定,因此可确保NGS实验获得稳定、可靠的结果。
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Available Products
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产品 Product no. 货号 目录价:
qPCR arrays (96-well format) for quantifying Illumina libraries constructed after target enrichment with the GeneRead DNAseq panels
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180601 NGIL-001Z varies
qPCR arrays (96-well format) for quantifying Ion Torrent libraries constructed after target enrichment with the GeneRead DNAseq panels
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180601 NGIT-001Z varies
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GeneRead DNAseq Library Quant Array适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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GeneRead DNAseq Library Quant Array的原理。
DNA标准品的梯度稀释液(5份十倍梯度稀释液)获得标准曲线。样本库应符合此标准曲线。
Principle
GeneRead DNAseq Library Quant Array通过real-time PCR技术,定量检测只有微乳液PCR(Ion Torrent)或桥式PCR(Illumina)可扩增的两端有接头的DNA,从而定量NGS文库。因此,能够高度准确的定量检测可扩增文库。如需确定实验样本的浓度,只需使用孔板中提供的5个十倍梯度稀释的DNA标准品,获得标准曲线,找到样本文库对应的值即可(参见Principle of QIAGEN GeneRead DNAseq Library Quant System)。real-time PCR灵敏性高,因此能扩增低浓度甚至是浓度低于传统分光光度法检测阈限的文库。

GeneRead DNAseq Library Quant Array还提供一套质控引物,用于监测扩增结果。每个GeneRead DNAseq Gene Panel都含有一套内对照;GeneRead DNAseq Library Quant Array提供引物,用于检测质控,确定制备的样本文库的QC得分。在测序前,可通过QC得分判断文库质量,去除质量差的文库,避免进行不必要、无法获得可靠结果的测序实验。GeneRead DNAseq Library Quant Array专为GeneRead qPCR SYBR® Green Mastermixes经过优化,具有出色的反应灵敏性和宽泛的动态范围。
Applications
GeneRead DNAseq Library Quant Array适用于定量文库并评定其质量,应配合GeneRead DNAseq Gene Panels使用。

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分析软件 (6)
New version – Excel-based data analysis template for use with GeneRead DNAseq Library Quant Arrays for 384-well plates for Illumina libraries
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New version – Excel-based data analysis template for use with GeneRead DNAseq Library Quant Arrays for 384-well plates for Ion Torrent libraries
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New version – Excel-based data analysis template for use with GeneRead DNAseq Library Quant Arrays for 96-well plates for Illumina® libraries
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New version – Excel-based data analysis template for use with GeneRead DNAseq Library Quant Arrays for 96-well plates for Ion Torrent™ libraries
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New version – Excel-based data analysis template for use with GeneRead DNAseq Library Quant Arrays for Rotor-Disc 100 rings for Illumina libraries

 


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New version – Excel-based data analysis template for use with GeneRead DNAseq Library Quant Arrays for Rotor-Disc 100 rings for Ion Torrent libraries

 


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试剂盒操作手册 (1)
New version – For reliable quantification of Ion Torrent or Illumina® libraries and target enrichment QC for GeneRead DNAseq Targeted Panels using real-time PCR
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产品介绍与指南 (2)
Accelerate your NGS performance through Sample to Insight solutions
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Accelerate your NGS performance through Sample to Insight solutions
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