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GeneRead DNAseq Gene Panels

用于在NGS实验前,对与某一通路相关的基因进行外显子靶向扩增
  • 为研究某一疾病或通路相关基因设计,提供预设计引物
  • 实验方案简便,适用于多种实验室PCR仪
  • 对于相关外显子进行靶向扩增
  • 提供免费的序列变化分析
  • 该产品提供内参,可在测序前评估DNA质量和扩增是否成功
GeneRead DNAseq Gene Panels能够对特定的一组基因进行可靠的遗传变化分析,适用于二代测序应用。该产品提供的多重PCR引物用于扩增研究所需的一组基因或与某种疾病相关的基因的外显子。GeneRead DNAseq Gene Panels还可依据您选择的基因提供定制产品,以满足您的实验需求。高品质的引物设计和靶向扩增试剂确保扩增的位点覆盖全面、无偏差、特异性高,保留您珍贵样本中即便是少量的遗传变异信息。
产品 Product no. 货号 目录价:
 
 
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Available Products
15
产品 Product no. 货号 目录价:
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
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181900 NGHS-001X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181900 NGHS-002X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181900 NGHS-003X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181900 NGHS-004X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181900 NGHS-005X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181900 NGHS-006X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181900 NGHS-007X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181900 NGHS-008X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181900 NGHS-009X varies
Sets of 4 tubes containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181901 NGHS-011X varies
Sets of 4 tubes containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181901 NGHS-013X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181900 NGHS-101X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181900 NGHS-102X varies
Sets of 1 tube containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181900 NGHS-201X varies
Sets of 4 tubes containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181901 NGHS-501X varies
Configure
产品 Product no. 货号 目录价:
 
 
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Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.

GeneRead DNAseq Gene Panels适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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GeneRead DNAseq Targeted Panel V2 workflow.
Genomic DNA (the QIAamp DNA Mini Kit, QIAamp DNA FFPE Tissue Kit, or GeneRead DNA FFPE Kit are recommended for extraction) can be quantified and qualified using the GeneRead DNA QuantiMIZE System. The GeneRead DNAseq Targeted Panels V2 are then used for targeted enrichment. Following target enrichment, construct your NGS library, quantify and quality-control using the GeneRead Library Quantification System, perform NGS, and analyze the data using the QIAGEN NGS Data Analysis Web Portal.
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Multiplex PCR-enabled target enrichment of genomic regions of interest
The principle of the GeneRead DNAseq V2 System is to employ overlapping primer sets across the exonic portions of a gene or genes to maximize target coverage and minimize nonspecific amplification.
Performance
通过检测位点覆盖率、扩增特异性和均一性,对GeneRead DNAseq Gene Panels的性能进行了评估。引物设计达到了90%以上的外显子覆盖。该产品的高度特异性确保测序时有85%以上为靶序列,高效利用测序实验。该产品的扩增位点均一性确保超过85%的碱基覆盖率,确保高品质的序列信息。
Principle
GeneRead DNAseq Gene Panel System使用单个基因或一组基因的外显子重叠引物,位点覆盖率高(参见Multiplex PCR-enabled target enrichment of genes of interest)。重叠引物分装在4个管中,因此提高了扩增特异性,避免扩增引物二聚体和非特异性产物。扩增和纯化后,将样本扩增的序列集合在一起,即制备获得该样本的文库。
Procedure
GeneRead DNAseq Gene Panels是二代测序完整工作流程的一部分(参见GeneRead DNAseq Gene Panel System targeted enrichment NGS workflow)。只需从您的样本中纯化DNA(推荐使用QIAamp DNA Mini Kit 或QIAamp DNA FFPE Tissue Kit),然后使用GeneRead DNAseq Gene Panels和GeneRead Panel Mastermix,通过PCR反应,对目标外显子进行扩增。扩增完成后,构建NGS文库,然后使用GeneRead DNAseq Library Quant Array对其进行定量分析和质量控制。之后可进行NGS实验,使用QIAGEN NGS Data Analysis Web Portal分析数据。
Applications
GeneRead DNAseq Gene Panels十分适合于扩增与癌症、遗传疾病、神经系统疾病、心肌病、发育失调、代谢失调等多个生物医学领域相关的基因,用于在后续NGS分析中确定遗传变化信息。

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产品介绍与指南 (2)
GeneRead Panels and NGS product configurator guide
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For targeted enrichment prior to next-generation sequencing
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分析软件 (3)
For analyzing, comparing, and visualizing next-generation sequencing data
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For basic analysis of NGS data using the GeneRead DNAseq panels V2; acceptable file formats include FASTQ or BAM
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For identifying disease-causing variants from human whole genome, exome, and gene panel next-generation sequencing studies
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试剂盒操作手册 (1)
All-bead protocol for targeted enrichment prior to next-generation sequencing
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