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QIAsymphony Virus/Bacteria Kits

从1–96个样本中自动纯化病毒核酸或细菌DNA

  • 标准化的处理流程保证高度重复性
  • 条形码扫描可追踪样本和试剂
  • 经优化的操作流程确保高产量高纯度的核酸
  • 新型预装试剂条,操作简便

QIAsymphony Virus/Bacteria Kit可在QIAsymphony SP核酸提取纯化分析仪上,从多种类型样本中自动纯化病毒核酸和细菌DNA。例如,可以从血浆、血清、脑脊液、呼吸道样本、尿常规样本、泌尿生殖道拭子和运输介质中纯化核酸。试剂盒有小型和中型规格,对应样本体积200 μl和至多1000 μl。经优化的实验方案提供高产量高纯度的核酸,和灵活的洗脱体积,因而可以根据每个下游应用,优化调整核酸浓度。新型的预装试剂条减少了工作站设置时间,并减少了手动操作。条形码阅读器进行试剂全程跟踪。

Cat No./ID: 931036
QIAsymphony Virus/Bacteria Mini Kit (192)
For 192 preps of 200 µl each: includes 2 reagent cartridges and enzyme racks and accessories

QIAsymphony Virus/Bacteria Kits适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


0
灵敏检测尿液中的Chlamydia trachomatis。
从800 μl尿液中纯化DNA,洗脱体积为110 μl。使用商品化的分析产品在ABI PRISM 7900HT SDS上扩增C. trachomatis基因组的特异性序列,检测细菌DNA。
1
Efficient processing of magnetic particles.
The QIAsymphony SP processes a sample containing magnetic particles as follows. A magnetic rod protected by a rod cover enters a well containing the sample and attracts the magnetic particles. The magnetic rod with cover is positioned above another well and releases the magnetic particles. The QIAsymphony SP uses a magnetic head containing an array of 24 magnetic rods, and can therefore process 24 samples simultaneously. Steps 1 and 2 are repeated several times during sample processing.
2
Prefilled, sealed reagent cartridges.
Reagent cartridges are prefilled with all reagents required for the purification procedure, including accessory enzymes, and are labeled with 2D bar codes. Partially used cartridges can be resealed for later use giving you complete cost control. 
Performance
QIAsymphony Virus/Bacteria Kit可以准确、重复地的纯化核酸。经纯化的病毒和细菌核酸没有蛋白质、核酸酶和其他杂质,适用于敏感的下游应用。高产量和灵活的洗脱体积(60 μl、85 μl或者110 μl)符合下游应用的要求,适用于敏感的分析检测(参见"Sensitive detection of Chlamydia trachomatis in urine")。
Principle
QIAsymphony Virus/Bacteria Kit将硅胶膜纯化病毒和细菌核酸的速度、效率与易于操作的磁性颗粒相结合(参见"Efficient processing of magnetic particles")。创新的即用型试剂条预装有磁性颗粒和纯化步骤所需的所有试剂,包括附加的酶,以及可在QIAsymphony SP核酸提取纯化分析仪上被自动打开的试剂,可较大程度降低污染的风险(参见"Prefilled, sealed reagent cartridges")。台面设置快速,节省你的宝贵时间。只需简单地在试剂置入模块中加载至多2个试剂条。试剂条可以来自相同的试剂盒,也可以是不同的试剂盒,因此可在处理96个样本的同一批次中,运行不同的纯化步骤。试剂仅会用于所选数目的样本,实现成本控制。
Procedure

纯化操作流程的目的在于确保安全、可重复的处理传染性样本,包括4个步骤:裂解、结合、洗涤和洗脱。用户可以选择不同的洗脱体积,取决于实验方案。在QIAsymphony SP核酸提取纯化分析仪上可完成所有纯化流程的步骤。每次运行至多可以处理96个样本。

QIAsymphony Virus/Bacteria实验方案
实验方案 试剂盒 样本材料处理体积
Cellfree 200   QIAsymphony Virus/Bacteria Mini Kit (192) 血浆、血清和脑脊液 200 μl
Cellfree 500    QIAsymphony Virus/Bacteria Midi Kit (96)  血浆、血清和脑脊液 500 μl
Cellfree 1000    QIAsymphony Virus/Bacteria Midi Kit (96)  血浆、血清和脑脊液 1000 μl
Complex 200   QIAsymphony Virus/Bacteria Mini Kit (192)   呼吸道样本(BAL、干拭子、传输介质、呼出物、痰)和泌尿生殖系统的样品(尿液、运输培养基)  200 μl
Complex 400   QIAsymphony Virus/Bacteria Midi Kit (96)   呼吸道样本(BAL、干拭子、传输介质、呼出物、痰)和泌尿生殖系统的样品(尿液、运输培养基) 400 μl 
Complex 800     QIAsymphony Virus/Bacteria Midi Kit (96) 呼吸道样本(BAL、干拭子、传输介质、呼出物、痰)和泌尿生殖系统的样品(尿液、运输培养基) 800 μl 
Applications

QIAsymphony Virus/Bacteria Kit能够灵敏的检测多种DNA和RNA病毒以及细菌病原体。产量呈线性,准确的定量分析高和低的病毒或者细菌滴度。高品质的核酸可即用于各种下游应用,包括定量real-time PCR或者RT-PCR等敏感的应用。

Features
Specifications
应用 PCR, real-time PCR
洗脱体积 60-110 µl
上样体积 800 µl (Pathogen Complex Protocol); 1000 µl (Virus Cellfree Protocol)
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 Purification of viral RNA and DNA and bacterial DNA
样本类型 Serum, plasma, CSF, cell-free body fluids, fresh or frozen material (body fluids, swabs, secretions, tissues, stool specimens)
技术 Magnetic particles