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Multi-Analyte ELISArray Kits

同时检测多个样本中多至12个细胞因子

  • 检测至多12个细胞因子或趋化因子
  • 高灵敏度和线性
  • 筛选、验证过的捕获和检测抗体
  • 使用标准的ELISA设备

Multi-Analyte ELISArray Kits是现有的最简单可同时进行多充分析的ELISA试剂盒。关键的细胞因子和趋化因子的高度特异性捕获抗体排列在96孔板中。简单的ELISA实验方案能够在多个样本中同时检测12个细胞因子和趋化因子。

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Multi-Analyte ELISArray Kit
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336161 MEH-003A 询价
Multi-Analyte ELISArray Kit
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336161 MEH-004A 询价
Multi-Analyte ELISArray Kit
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336161 MEH-005A 询价
Multi-Analyte ELISArray Kit
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336161 MEH-006A 询价
Multi-Analyte ELISArray Kit
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336161 MEH-007A 询价
Multi-Analyte ELISArray Kit
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336161 MEH-008A 询价
Multi-Analyte ELISArray Kit
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336161 MEH-009A 询价
Multi-Analyte ELISArray Kit
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336161 MEM-003A 询价
Multi-Analyte ELISArray Kit
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336161 MEM-004A 询价
Multi-Analyte ELISArray Kit
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336161 MEM-005A 询价
Multi-Analyte ELISArray Kit
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336161 MEM-006A 询价
Multi-Analyte ELISArray Kit
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336161 MEM-007A 询价
Multi-Analyte ELISArray Kit
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336161 MEM-008A 询价
Multi-Analyte ELISArray Kit
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336161 MEM-009A 询价
Multi-Analyte ELISArray Kit
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336161 MER-004A 询价
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Multi-Analyte ELISArray Kits适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


0
ELISArray protocol.

Multi-Analyte ELISArray Kits follow a traditional ELISA protocol that can be completed in any laboratory with a microplate reader.
1
Multi-Analyte ELISArray plate layout.

Each Multi-Analyte ELISArray contains twelve 8-well strips (represented by colored columns) coated with a specific capture antibody for a cytokine or chemokine. Six samples can be analyzed at one time, in addition to controls.
2
Patterns of Th1/Th2 cytokine induction.

Th1/Th2 cytokines were induced by human peripheral blood mononuclear cells in response to PMA (50 µg/ml) and ionomycin (1 µg/ml). Time-dependent patterns of induced cytokines and relative cytokine amounts were profiled and plotted.

3
Optimized ELISA conditions enable the same development or incubation time.

Similar standard curves are observed for all optimized cytokine and chemokine assays in the Multi-Analyte ELISArray Kits. All standard curves are virtually superimposable indicating that all 12 assays provide similar linearity and sensitivity under the same standardized conditions and development or incubation times.

Performance

Multi-Analyte ELISArray Kit应用的示例如下:Multi-Analyte ELISArray Kit用于测量多由刺激人外周血单个核细胞而分泌的多个细胞因子(PBMC)。可监控由人类PBMC诱导的8个Th1/Th2细胞因子的时间决定模式(0、6、18、24和48小时)以应对PMA(50 µg/ml)和离子霉素(1 µg/ml)(参见"Patterns of Th1/Th2 cytokine induction")。通过使用Multi-Analyte ELISArray Kit也可对8个细胞因子的相对量进行测量。

可整体诱导大量的IL-2和IFNγ,以及程度较轻的TNFα,且显然超出所测定的时间。只有诱导更多适量的IL4和IL5诱导,才可经约24小时达到最大刺激。甚至更低量的IL10和IL13也可在24小时达到分泌最大量,而且稍后会再次降低。在48小时的持续刺激后,仍检测不到IL12的量。

Principle

Multi-Analyte ELISArray Kits应用简单的实验方案和标准的开发时间测量6个细胞上清液(媒介)、血清或血浆样本中的12个细胞因子(参见"Multi-Analyte ELISArray plate layout")。Multi-Analyte ELISArray Kits能为研究者提供对多个样本中多种蛋白质水平的快速评估(细胞因子和趋化因子)。

筛选市售的捕获和检测抗体用于获得尽可能高的灵敏度和线性契合度,用于每个细胞因子或趋化因子的检测。在同一的条件下对所有检测进行优化,在不影响性能的同时进行检测(参见"Optimized ELISA conditions enable the same development or incubation time")。

Procedure

Multi-Analyte ELISArray Kits使用标准的ELISA三明治操作方法(参见"ELISArray protocol")。无需特殊设备;该试剂盒可与任何标准ELISA酶标仪兼容。

Applications

Multi-Analyte ELISArray Kits高度适用于研究自身免疫性和免疫系统疾病、癌症、免疫学、传染病以及其他领域的生物和医学研究。试剂盒可用于应用ELISA试剂盒进行常规分析的各种应用。