用于常规和特殊的PCR
- QIAGEN PCR缓冲体系最大限度地减少了PCR条件的优化
- 独特的即用型PCR缓冲液，操作更快速简单
- Q-Solution可帮助扩增GC含量高的模板
- 提供多种规格的包装方便使用
QIAGEN PCR Buffer中的Taq DNA Polymerase最大限度的减少了使用时对PCR条件的优化，新型的辅助剂Q-Solution则有助于实现“困难”模板（比如，GC含量高的模板）的高效扩增。CoralLoad PCR Buffer（包含两种胶示踪染料）使PCR产物可以直接上样电泳。
fr-FR
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Product
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Cat. no.
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List price:
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Taq DNA Polymerase (250 U)
250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
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201203
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Taq DNA Polymerase (1000 U)
4 x 250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
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201205
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Taq DNA Polymerase (5000 U)
20 x 250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
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201207
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Taq DNA Polymerase Kit (25000 U)
100 x 250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
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201209
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The Taq DNA Polymerase is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
CoralLoad PCR Buffer.|Tolerance to variable magnesium concentration.|Amplification of difficult templates.|Tolerance of different primer Tm values.|Lot-to-lot reproducibility.|Specific amplification of long PCR products.|Increased specificity of primer annealing.|
[A] CoralLoad Concentrate contains two gel-tracking dyes, enabling immediate gel loading of PCR products for [B ] easy visualization of DNA migration.|PCR amplification at the indicated Mg2+ concentrations using QIAGEN PCR Buffer and Taq DNA Polymerase (QIAGEN). The same PCR was performed in parallel using a PCR buffer and Taq polymerase from another supplier (Supplier AII). The single-copy human prion protein gene was amplified successfully in each case using the buffer and enzyme from QIAGEN. M: markers.|Two different primer-template systems were amplified in duplicate using QIAGEN PCR Buffer and Taq DNA Polymerase in the absence (–) or presence (+) of 1x Q-Solution. Q-Solution enables specific amplification of difficult templates. [A] human angiotensin receptor II gene; [B] mouse protein kinase C gene; M: markers.|The human single-copy cystic fibrosis gene was amplified with Taq DNA Polymerase and QIAGEN PCR Buffer using the indicated annealing temperatures. Primers employed were a 22mer with a Tm of 57.5°C (GC content: 54.5%) and a 32mer with a Tm of 85.2°C (GC content: 78%). For analysis, 10% of a 100 µl reaction was loaded. M: markers.|A fragment of the single-copy gene for cystic fibrosis was amplified from 30 ng, 3 ng, and 300 pg human genomic DNA corresponding to 104, 103, and 102 copies of target template, respectively. Three different lots of Taq DNA Polymerase were used and equal volumes of the PCR product were analyzed on a 1% agarose gel. M: markers.|Three different-sized products from human genomic DNA were amplified using either Taq DNA Polymerase and QIAGEN PCR Buffer (QIAGEN), or Taq polymerase and a buffer from another supplier (Supplier AII). For analysis, 10% of each reaction was loaded on the gel. Results from duplicate PCR amplifications are shown. M: markers.|Ammonium and potassium cations in QIAGEN PCR Buffers increase specificity of primer annealing. K+ binds to the phosphate groups (P–) on the DNA backbone, stabilizing the annealing of the primers to the template. NH4+, which exists both as the ammonium ion and as ammonia under thermal-cycling conditions, can interact with the hydrogen bonds between the bases (B), destabilizing the weak hydrogen bonds at mismatched bases. The combined effect of the two cations maintains the high ratio of specific-to-nonspecific primer-template binding over a wide temperature range.|
Procedure
Taq DNA Polymerase可在多种应用中进行高度特异性的PCR，减少PCR参数的优化过程。该试剂盒简化的、易于操作的流程使PCR反应体系构建更加简单。CoralLoad PCR Buffer也增加便利性和易操作性。PCR产物可直接上样到凝胶中，无需添加上样染料。可提升PCR性能的Q-Solution确保成功处理GC含量高的模板。
Applications
Taq DNA Polymerase适用于常规和特殊的应用，包括：
- 常规PCR
- RT-PCR
- 筛选
- 基于PCR的DNA指纹图谱分析（VNTR、STR和RAPD）
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Feature
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Specifications
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Applications
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PCR, RT-PCR, DNA fingerprinting
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dNTP's included
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No
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Enzyme activity
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5' -> 3' exonuclease activity
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Mastermix
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No
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Reaction type
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PCR amplification
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Real-time or endpoint
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Endpoint
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Sample/target type
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Genomic DNA and cDNA
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Single or multiplex
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Single
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With/without hotstart
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Without hotstart
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For standard and specialized PCR applications with minimal optimization
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Show details
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Download Safety Data Sheets for QIAGEN product components.
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View
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Images
CoralLoad PCR Buffer.
[A] CoralLoad Concentrate contains two gel-tracking dyes, enabling immediate gel loading of PCR products for [B ] easy visualization of DNA migration.
Tolerance to variable magnesium concentration.
PCR amplification at the indicated Mg2+ concentrations using QIAGEN PCR Buffer and Taq DNA Polymerase (QIAGEN). The same PCR was performed in parallel using a PCR buffer and Taq polymerase from another supplier (Supplier AII). The single-copy human prion protein gene was amplified successfully in each case using the buffer and enzyme from QIAGEN. M: markers.
Amplification of difficult templates.
Two different primer-template systems were amplified in duplicate using QIAGEN PCR Buffer and Taq DNA Polymerase in the absence (–) or presence (+) of 1x Q-Solution. Q-Solution enables specific amplification of difficult templates. [A] human angiotensin receptor II gene; [B] mouse protein kinase C gene; M: markers.
Tolerance of different primer Tm values.
The human single-copy cystic fibrosis gene was amplified with Taq DNA Polymerase and QIAGEN PCR Buffer using the indicated annealing temperatures. Primers employed were a 22mer with a Tm of 57.5°C (GC content: 54.5%) and a 32mer with a Tm of 85.2°C (GC content: 78%). For analysis, 10% of a 100 µl reaction was loaded. M: markers.
Lot-to-lot reproducibility.
A fragment of the single-copy gene for cystic fibrosis was amplified from 30 ng, 3 ng, and 300 pg human genomic DNA corresponding to 104, 103, and 102 copies of target template, respectively. Three different lots of Taq DNA Polymerase were used and equal volumes of the PCR product were analyzed on a 1% agarose gel. M: markers.
Specific amplification of long PCR products.
Three different-sized products from human genomic DNA were amplified using either Taq DNA Polymerase and QIAGEN PCR Buffer (QIAGEN), or Taq polymerase and a buffer from another supplier (Supplier AII). For analysis, 10% of each reaction was loaded on the gel. Results from duplicate PCR amplifications are shown. M: markers.
Increased specificity of primer annealing.
Ammonium and potassium cations in QIAGEN PCR Buffers increase specificity of primer annealing. K+ binds to the phosphate groups (P–) on the DNA backbone, stabilizing the annealing of the primers to the template. NH4+, which exists both as the ammonium ion and as ammonia under thermal-cycling conditions, can interact with the hydrogen bonds between the bases (B), destabilizing the weak hydrogen bonds at mismatched bases. The combined effect of the two cations maintains the high ratio of specific-to-nonspecific primer-template binding over a wide temperature range.
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