Rotor-Gene SYBR^® Green PCR Kit

在Rotor-Gene PCR仪上使用SYBR Green I通过两步法qRT-PCR超快速进行基因表达分析

特异性检测低拷贝靶基因
精确检测广泛模板量范围内的基因
特制的即用型预混液，用于快速循环
配合QuantiTect Primer Assays使用，可保证性能

Rotor-Gene SYBR Green PCR Kit专用于Rotor-Gene Q实时荧光定量PCR分析仪和其他的Rotor-Gene PCR仪，使用SYBR Green I对cDNA靶基因进行超快速、高特异性的real-time PCR和两步法RT-PCR分析。经优化的预混液与独特的Rotor-Gene PCR仪配合使用保证了qPCR良好的性能。预混液可贮存在2–8°C，方便使用。

订购信息
详细信息
技术参数
相关资料

请登录账号查看购物车

产品		货号	目录价：
Rotor-Gene SYBR Green PCR Kit (400) For 400 x 25 µl reactions: 3 x 1.7 ml 2x Rotor-Gene SYBR Green PCR Master Mix, 2 x 2 ml RNase-Free Water		204074	询价

请登录账号查看购物车

Rotor-Gene SYBR^® Green PCR Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。

Main Image Navi

高灵敏度、高特异性的检测。|可靠、特异性的检测，可检测低达10个拷贝。|Specific primer annealing.|Fast primer annealing.|

使用10倍稀释的人白细胞cDNA（10 ng到0.1 ng）和QuantiTect Primer Assay对BCL2（CLL/2型淋巴瘤B细胞）进行real-time两步法RT-PCR。反应使用[A] Rotor-Gene SYBR^® Green PCR Kit和Rotor-Gene Q实时荧光定量PCR分析仪或[B]供应商A_II提供的试剂盒和循环仪进行，重复三次实验。Rotor-Gene体系能够检测到较低的C_T值并且有更好的重复性。熔解曲线分析只有一个单峰，表明靶向扩增的特异性。|使用10倍稀释的质粒DNA（10⁹稀释到10个拷贝）和QuantiTect Primer Assay对PPIA（亲环素A）进行real-time PCR。反应使用Rotor-Gene SYBR^® Green PCR Kit和Rotor-Gene Q实时荧光定量PCR分析仪，反应重复三次。[A] Rotor-Gene体系在整个浓度范围内检测可靠且C_T值重复性好。[B]熔解曲线分析表明靶向扩增的特异性。|Cations in the Rotor-Gene Q PCR buffer increase specific primer annealing. K⁺ binds to phosphate groups on double-stranded DNA, stabilizing primer annealing. NH₄⁺ destabilizes weak hydrogen bonds between mismatched bases.|[A] Q-Bond in Rotor-Gene SYBR^® Green PCR Master Mix increases the affinity of DNA polymerase for short single-stranded DNA, reducing primer annealing time to a few seconds. In addition, the unique buffer composition supports the melting of DNA, reducing denaturation and extension times. [B] Without Q-Bond, the primer and polymerase bind sequentially to the template, increasing primer annealing time.|

性能

Rotor-Gene SYBR Green PCR Kit特别适用于对cDNA靶基因进行基因表达分析（参见"Highly specific and sensitive detection"和"Reliable and specific detection down to 10 copies"）。

QuantiTect Primer Assays与Rotor-Gene SYBR Green PCR Kit配合使用，可高灵敏度定量检测特异性PCR产物，无需经过优化（参见下表）。

使用SYBR Green在RT-PCR中获得优越性能
	QIAGEN		Supplier A_II
	C_T	平均偏差	C_T	平均偏差
BAX (BCL2-associated X protein)	24.84	0.05	29.57	0.46
BCL2 (凋亡基因)	26.96	0.05	32.83	0.29
MYC (原癌细胞)	28.42	0.21	35.26	0.72
b-Actin (管家基因)	20.24	0.03	24.39	0.12

原理

Rotor-Gene SYBR Green PCR Kit可在Rotor-Gene Q实时荧光定量PCR分析仪上进行快速、可靠的real-time PCR定量分析，无需优化反应及循环条件。混合液中的荧光染料SYBR Green I能用于分析多种不同的靶基因而无需合成靶标特异性标签探针。预混液中浓度平衡的K⁺和NH₄⁺离子组合保证了高特异性扩增，使引物退火高度特异性，获得高PCR特异性和灵敏性（参见"Specific primer annealing"）。使用创新的PCR辅助剂Q-Bond使循环时间降低至45分钟，达到快速分析且不降低性能（参见"Fast primer annealing"）。

2x Rotor-Gene SYBR Green PCR Kit的组分*
组分	特点	优势
HotStarTaq Plus DNA Polymerase	在95ºC下5分钟活化	室温下进行qPCR反应体系构建
Rotor-Gene SYBR Green PCR Buffer	浓度平衡的K⁺和NH₄⁺离子组合	引物特异性结合保证可靠的qPCR结果
Rotor-Gene SYBR Green PCR Buffer	独特的Q-Bond添加剂	快速的PCR运行时间可获得较快的结果和每天更多的反应次数
SYBR Green I染料	与双链DNA结合产生较强的荧光信号	高度灵敏的定量检测

操作流程

Rotor-Gene SYBR Green PCR Master Mix无需优化反应及循环条件。只需将cDNA模板DNA与引物加入到预混液中并设置循环程序。在试剂盒使用手册中有详细说明。

对于基于real-time两步法RT-PCR的基因表达分析，Rotor-Gene SYBR Green PCR Kit、QuantiTect Reverse Transcription Kit、QuantiTect Primer Assays及Rotor-Gene Q实时荧光定量PCR分析仪提供了完成的即用型解决方案。QuantiTect Reverse Transcription Kit可以在20分钟内快速合成cDNA并完全去除基因组DNA污染。QuantiTect Primer Assays为覆盖全基因组，经功效验证的预制引物对，用于检测来自人、小鼠、大鼠和其他物种的转录本。QuantiTect Primer Assays可以便利的在GeneGlobe网上订购。

应用

Rotor-Gene SYBR Green PCR Kit适用于在Rotor-Gene Q实时荧光定量PCR分析仪上对cDNA靶基因进行快速real-time定量分析。该试剂盒与Rotor-Gene 3000和Rotor-Gene 6000兼容。

可使用Rotor-Gene SYBR Green RT-PCR Kit在Rotor-Gene PCR仪上通过SYBR Green I对目标RNA进行超快速、一步法qRT-PCR基因表达分析。

特点	参数
应用	Real-Time quantification of genomic DNA or cDNA targets
描述	For ultrafast quantitative real-time PCR and two-step RT-PCR using SYBR Green I on Rotor-Gene cyclers
反应类型	Real-time PCR and two-step RT-PCR
real-time或终点法PCR	Real-time
样本/目标类型	DNA, cDNA
单一或多重	Single
SYBR Green I或序列特异性探针	SYBR Green I
热循环仪	Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000
有/无ROX	Without ROX dye

产品介绍与指南

分类选项按字母顺序排序
	(EN) - Rotor-Gene Q - Pure Detection Now with even more applications!	显示更多

常见问题

分类选项按字母顺序排序
	What is a QuantiTect Primer Assay? FAQ ID -1141	浏览
	How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling? FAQ ID -1430	浏览
	Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits? FAQ ID -2104	浏览
	Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument? FAQ ID -2116	浏览
	Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment? FAQ ID -2117	浏览
	Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits? FAQ ID -2118	浏览
	What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits? FAQ ID -2119	浏览
	Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers? FAQ ID -2121	浏览
	Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits? FAQ ID -2122	浏览
	Do QuantiTect Primer Assays also work with Rotor-Gene SYBR Green PCR Kits? FAQ ID -2123	浏览
	Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC? FAQ ID -2124	浏览
	Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? FAQ ID -2371	浏览
	How do I create a workspace that is free of DNA contamination, prior to carrying out a qPCR experiment? FAQ ID -2654	浏览
	What real-time cycler should I use for my qPCR experiments? FAQ ID -2670	浏览
	What is a dissociation curve, and why is it important to run a dissociation curve, following qPCR using SYBR Green chemistry? FAQ ID -2678	浏览
	What is the threshold cycle or Ct value? FAQ ID -2682	浏览
	How can I predict the percent qPCR signal due to contaminating DNA, for a given qPCR assay, and its matching NRT control? FAQ ID -2688	浏览
	Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC? FAQ ID -2690	浏览

试剂盒操作手册

分类选项按字母顺序排序
	(EN) - Rotor-Gene SYBR Green Handbook For fast real-time PCR, two-step RT-PCR, and one-step RT-PCR using SYBR Green I on Rotor-Gene cyclers	显示更多
	(EN) - QuantiTect Primer Assay Handbook For genomewide, ready-to-use real-time RT-PCR assays using SYBR Green detection	显示更多

技术资讯

分类选项按字母顺序排序
	(EN) - Critical factors for synthesis of cDNA for real-time PCR analysis	显示更多

补充实验方案

分类选项按字母顺序排序
	Rotor-Gene® software setup for the Rotor-Gene SYBR® Green PCR Kit - (EN)	显示更多

安全数据表

分类选项按字母顺序排序
	Safety Data Sheets Download Safety Data Sheets for QIAGEN product components.	浏览

安全数据表

分类选项按字母顺序排序
	MSDS Rotor-Gene SYBR Green PCR Kit (400)

图片

Highly specific and sensitive detection.

高灵敏度、高特异性的检测。

Reliable and specific detection down to 10 copies.

可靠、特异性的检测，可检测低达10个拷贝。

使用10倍稀释的质粒DNA（10⁹稀释到10个拷贝）和QuantiTect Primer Assay对PPIA（亲环素A）进行real-time PCR。反应使用Rotor-Gene SYBR^® Green PCR Kit和Rotor-Gene Q实时荧光定量PCR分析仪，反应重复三次。[A] Rotor-Gene体系在整个浓度范围内检测可靠且C_T值重复性好。[B]熔解曲线分析表明靶向扩增的特异性。

Specific primer annealing.

Cations in the Rotor-Gene Q PCR buffer increase specific primer annealing. K⁺ binds to phosphate groups on double-stranded DNA, stabilizing primer annealing. NH₄⁺ destabilizes weak hydrogen bonds between mismatched bases.

Fast primer annealing.

[A] Q-Bond in Rotor-Gene SYBR^® Green PCR Master Mix increases the affinity of DNA polymerase for short single-stranded DNA, reducing primer annealing time to a few seconds. In addition, the unique buffer composition supports the melting of DNA, reducing denaturation and extension times. [B] Without Q-Bond, the primer and polymerase bind sequentially to the template, increasing primer annealing time.

Rotor-Gene SYBR® Green PCR Kit

Rotor-Gene SYBR^® Green PCR Kit