RNAprotect Tissue Reagent

立即稳定组织中的RNA

  • 立即稳定和保护RNA
  • 可靠的基因表达和分析数据
  • 室温下操作方便安全
  • 无需使用液氮或干冰
  • 可长期保存组织,无RNA降解风险
RNAprotect Tissue Reagent立即稳定组织样本中的RNA以保存基因表达谱,提供50 ml或250 ml两种规格。可使用Allprotect Tissue Reagent稳定组织样本中的DNA、RNA和蛋白质。
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Product Cat. no. List price:
RNAprotect Tissue Reagent (50 ml)
50 ml RNAprotect Tissue Reagent for stabilization of RNA in 25 x 200 mg tissue samples
76104
80,30 €
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RNAprotect Tissue Reagent (250 ml)
250 ml RNAprotect Tissue Reagent for stabilization of RNA in 125 x 200 mg tissue samples
76106
331,00 €
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RNAprotect Reagent prevents degradation of mRNA in tissues.|
RNA was isolated from fresh rat kidney samples after 0, 5, 10, 15, 30, and 60 minutes, using either standard procedures (Unstabilized) or RNeasy Protect Kits (Stabilized). The RNA isolated was analyzed by agarose gel electrophoresis and expression of GAPDH was examined using northern blot analysis. M: markers.|
Performance
RNAprotect Tissue Reagent可稳定和保护在收集和储存样本时的RNA表达谱(参见"RNAlater Reagent prevents degradation of mRNA in tissues")。RNAlater技术对RNA的立即保护,可确保下游分析真实地反映整个组织的表达谱(参见"TissueProtect Tubes prevent RNA changes in tissueslater RNA")。样本可长期保存,无RNA降解风险,甚至经多次冻融后也无影响。在从多种条件下保存的RNAlater稳定样本中纯化得到的RNA,适用于northern印迹和RT-PCR等下游应用(参见"Stable RNA in tissues: different temperatures and freeze–thaw cycles")。
Principe

一旦生物样本被收集后,其RNA会变得极不稳定。创新的液状RNAprotect Tissue Reagent可快速渗入组织样本,立即稳定RNA,保护RNA表达模式。还能避免样本处理导致的基因诱导或下调,可进行准确的基因表达分析。

Procédure

组织样本收集后,迅速浸入适当体积的RNAprotect Tissue Reagent中,即可保护RNA和储存样本。用可重复密封的管子,RNAprotect Tissue Tubes可预先测定所需RNAprotect Tissue Reagent的体积,方便操作和样本保存。该试剂可使RNA在37°C下保存1天,18–25°C下保存7天,2–8°C下保存4周,并且可以在无液氮或干冰条件下处理、运输和储存样本。另外,样本可以在–20°C或–80°C条件下存档。

Applications

使用RNAprotect Tissue Reagent稳定后的动物和人体组织样本,可用QIAGEN仪器进行高效的样本破碎,并配合QIAGEN试剂盒用于:

总RNA纯化
miRNA纯化
mRNA纯化

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Kit Handbooks
1
RNAprotect Tissue Tubes - For collection of harvested animal tissues with immediate stabilization of the gene expression profile, and subsequent transport and storage; RNAprotect Tissue Reagent - For immediate stabilization of the gene expression profile in harvested animal tissues
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Images
RNAlater Reagent prevents degradation of mRNA in tissues.
RNAprotect Reagent prevents degradation of mRNA in tissues.
RNA was isolated from fresh rat kidney samples after 0, 5, 10, 15, 30, and 60 minutes, using either standard procedures (Unstabilized) or RNeasy Protect Kits (Stabilized). The RNA isolated was analyzed by agarose gel electrophoresis and expression of GAPDH was examined using northern blot analysis. M: markers.