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RT2 SYBR® Green qPCR Mastermixes

用于real-time PCR分析

  • 特异性扩增,引物二聚体较少
  • 一体化混合物,适用于特定仪器
  • 可选含有ROX、荧光素或不含染料的规格
RT2 SYBR Green qPCR Mastermixes非常适合使用SYBR Green进行real-time PCR分析,可选含有或不含参比染料的规格。RT2 SYBR Green qPCR Mastermixes应与RT2 qPCR Primer Assays和RT2 Profiler PCR Arrays配合使用。
Cat No./ID: 330500
RT² SYBR Green qPCR Mastermix (2)
Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Arrays or 200 x 25 µl reactions or 400 x 10 µl reactions
Cat No./ID: 330501
RT² SYBR Green qPCR Mastermix (8)
Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactions or 2000 x 10 µl reactions
Cat No./ID: 330502
RT² SYBR Green qPCR Mastermix (12)
Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactions or 3000 x 10 µl reactions
Cat No./ID: 330503
RT² SYBR Green qPCR Mastermix (24)
Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactions or 6000 x 10 µl reactions
Cat No./ID: 330504
RT² SYBR Green qPCR Mastermix (6)
Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Arrays or 600 x 25 µl reactions or 1500 x 10 µl reactions
Cat No./ID: 330509
RT² SYBR Green qPCR Mastermix (25 ml)
Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactions or 5000 x 10 µl reactions
Cat No./ID: 330510
RT² SYBR Green Fluor qPCR Mastermix (2)
Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Array or 200 x 25 µl reactions or 400 x 10 µl reactions
Cat No./ID: 330511
RT² SYBR Green Fluor qPCR Mastermix (8)
Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactions or 2000 x 10 µl reactions
Cat No./ID: 330512
RT² SYBR Green Fluor qPCR Mastermix (12)
Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactions or 3000 x 10 µl reactions
Cat No./ID: 330513
RT² SYBR Green Fluor qPCR Mastermix (24)
Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactions or 6000 x10 µl reactions
Cat No./ID: 330514
RT² SYBR Green Fluor qPCR Mastermix (6)
Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Array or 600 x 25 µl reactions or 1500 x 10 µl reactions
Cat No./ID: 330519
RT² SYBR Green Fluor qPCR Mastermix (25 ml)
Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactions or 5000 x 10 µl reactions
Cat No./ID: 330520
RT² SYBR Green ROX qPCR Mastermix (2)
Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Array or 200 x 25 µl reactions or 400 x 10 µl reactions
Cat No./ID: 330521
RT² SYBR Green ROX qPCR Mastermix (8)
Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactions or 2000 x 10 µl reactions
Cat No./ID: 330522
RT² SYBR Green ROX qPCR Mastermix (12)
Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactions or 3000 x 10 µl reactions
Cat No./ID: 330523
RT² SYBR Green ROX qPCR Mastermix (24)
Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactions or 6000 x 10 µl reactions
Cat No./ID: 330524
RT² SYBR Green ROX qPCR Mastermix (6)
Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Array or 600 x 25 µl reactions or 1500 x 10 µl reactions
Cat No./ID: 330529
RT² SYBR Green ROX qPCR Mastermix (25 ml)
Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactions or 5000 x 10 µl reactions

RT2 SYBR® Green qPCR Mastermixes适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


0
High amplification efficiency over a wide dynamic range.
Using duplicate 10-fold serial dilutions of purified template and the RT2 qPCR Primer Assay for the Human Nicotinic Acetylcholine Receptor Alpha 5 (CHRNA5), RT2 SYBR® Green Mastermixes demonstrate a 6-log linear dynamic range.
1
Tighter control of polymerase activity yields greater specificity.
Greater specificity was achieved using RT2 SYBR® Green Mastermixes [A] compared to master mixes from an alternative supplier [B].
Performance

RT² SYBR Green qPCR Mastermixes包含所有优化的试剂和缓冲液,可在各种real-time PCR分析仪上配合RT2 qPCR Primer Assays和RT2 Profiler PCR Arrays进行SYBR Green real-time PCR分析。

RT² SYBR Green qPCR Mastermixes进行的高性能real-time PCR具有较高的扩增效率(参见"High amplification efficiency over a wide dynamic range")和高灵敏度及特异性(参见"Tighter control of polymerase activity yields greater specificity")。

Principle

RT2 SYBR Green qPCR Mastermixes包含real-time PCR缓冲液、高性能的HotStart DNA Taq聚合酶、核苷酸和SYBR Green染料。有些预混液包含荧光素或ROX染料,用于优化光学仪器。化学修饰并严格控制的HotStart酶可避免扩增引物二聚体和其他非特异性产物,提供准确的SYBR Green结果。

不含ROX或荧光素的预混液

RT2 SYBR Green qPCR Mastermix适合在不需要参比染料的仪器上进行SYBR Green real-time PCR应用,仪器包括Bio-Rad models CFX96、CFX384;Bio-Rad/MJ Research Chromo4、DNA Engine Opticon、DNA Engine Opticon 2;Roche LightCycler 480(96孔和384孔);无ROX滤过装置的Eppendorf Mastercycler ep realplex和Cepheid SmartCycler。

含有荧光素的预混液

RT2 SYBR Green qPCR Mastermix适合在需要荧光素作为参比染料的仪器上进行SYBR Green real-time PCR应用,仪器包括Bio-Rad models iCycler、iQ5、MyiQ和MyiQ2。

含有ROX的预混液

RT2 SYBR Green qPCR Mastermix适合在需要ROX作为参比染料的仪器上进行SYBR Green real-time PCR应用,仪器包括QIAGEN的Rotor-Gene Q实时荧光定量PCR分析仪;Applied Biosystems models 5700、7000、7300、7500(标准和快速)、7700、7900HT(标准和快速的96-well block,384-well block)、StepOnePlus和ViiA 7(标准和快速的96-well block,384-well block);含有或无ROX滤过装置的Eppendorf Mastercycler ep realplex;Stratagene models Mx3000P、Mx3005P、Mx4000以及Takara TP-800。

Applications
RT2 SYBR Green qPCR Mastermixes适合real-time PCR分析,特别是RT2 qPCR Primer Assays和RT2 Profiler PCR Arrays。

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试剂盒操作手册 (1)
仪器技术参数 (1)
For pathway-focused gene expression analysis
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