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RT2 SYBR® Green FAST Mastermixes

用于real-time PCR分析

  • 特异性扩增,引物二聚体较少
  • 一体化混合物,适用于特定仪器
  • 可选含有ROX、荧光素或不含染料的规格
  • 实验步骤比常规SYBR Green预混液的步骤少
  • 灵敏度高、解离曲线好
RT2 SYBR Green FAST Mastermixes非常适合使用SYBR Green进行real-time PCR分析,可选含有或不含参比染料的规格。RT2 SYBR Green FAST Mastermixes经优化后具有更快的PCR操作流程,而不影响灵敏度和特异性。RT2 SYBR Green FAST Mastermixes应与RT2 qPCR Primer Assays和RT2 Profiler PCR Arrays配合使用。
Cat No./ID: 330600
RT² SYBR Green FAST Mastermix (2)
Contains 2 x 1.35 ml tubes: for 12 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 250 x 20 µl reactions
Cat No./ID: 330601
RT² SYBR Green FAST Mastermix (8)
Contains 8 x 1.35 ml tubes: for 8 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 1000 x 20 µl reactions
Cat No./ID: 330602
RT² SYBR Green FAST Mastermix (12)
Contains 12 x 1.35 ml tubes: for 12 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 1500 x 20 µl reactions
Cat No./ID: 330603
RT² SYBR Green FAST Mastermix (24)
Contains 24 x 1.35 ml tubes: for 24 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 3000 x 20 µl reactions
Cat No./ID: 330609
RT² SYBR Green FAST Mastermix (25 ml)
Contains 1 bottle of 25 ml mastermix: for 24 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 2500 x 20 µl reactions
Cat No./ID: 330610
RT² SYBR Green Fluor FAST Mastermix (2)
Contains 2 x 1.35 ml tubes: for 12 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 250 x 20 µl reactions
Cat No./ID: 330611
RT² SYBR Green Fluor FAST Mastermix (8)
Contains 8 x 1.35 ml tubes: for 8 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 1000 x 20 µl reactions
Cat No./ID: 330612
RT² SYBR Green Fluor FAST Mastermix (12)
Contains 12 tubes of 1.35 ml each: for 12 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 1500 x 20 µl reactions
Cat No./ID: 330613
RT² SYBR Green Fluor FAST Mastermix (24)
Contains 24 x 1.35 ml tubes: for 24 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 3000 x 20 µl reactions
Cat No./ID: 330619
RT² SYBR Green Fluor FAST Mastermix (25 ml)
Contains 1 bottle of 25 ml mastermix: for 24 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 2500 x 20 µl reactions
Cat No./ID: 330620
RT² SYBR Green ROX FAST Mastermix (2)
Contains 2 x 1.35 ml tubes: for 12 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 250 x 20 µl reactions
Cat No./ID: 330621
RT² SYBR Green ROX FAST Mastermix (8)
Contains 8 x 1.35 ml tubes: for 8 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 1000 x 20 µl reactions
Cat No./ID: 330622
RT² SYBR Green ROX FAST Mastermix (12)
Contains 12 x 1.35 ml tubes: for 12 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 1500 x 20 µl reactions
Cat No./ID: 330623
RT² SYBR Green ROX FAST Mastermix (24)
Contains 24 x 1.35 ml tubes: for 24 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 3000 x 20 µl reactions
Cat No./ID: 330629
RT² SYBR Green ROX FAST Mastermix (25 ml)
Contains 1 bottle of 25 ml mastermix: for 24 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 2500 x 20 µl reactions

RT2 SYBR® Green FAST Mastermixes适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


0
使用RT2 SYBR® Green FAST Mastermix获得更清晰的熔解曲线。
在ABI 7500 qRT-PCR仪上使用[A]常规PCR和RT² SYBR Green Mastermix或[B]快速PCR和RT² SYBR Green FAST Mastermix扩增PCR阳性对照。使用RT² SYBR Green FAST Mastermix获得的熔解曲线更清晰。
1
在宽泛的动态范围内,具有高扩增效率。
使用10倍梯度稀释的纯化模板和Human Nicotinic Acetylcholine Receptor Alpha 5 (CHRNA5)的RT2 qPCR Primer Assay进行两次重复实验,RT2 SYBR® Green Mastermixes表现出高扩增效率。
2
更好的控制聚合酶活性,达到更高特异性。
[B]其他供应商提供的预混液相比[A]RT2 SYBR® Green Mastermixes有更高的特异性。
Performance

RT² SYBR Green FAST Mastermixes包含所有优化的试剂和缓冲液,可在各种real-time PCR分析仪上配合RT2 qPCR Primer Assays和RT2 Profiler PCR Arrays进行SYBR Green real-time PCR分析。

RT² SYBR Green FAST Mastermixes进行的高性能real-time PCR具有较高的扩增效率(参见"High amplification efficiency over a wide dynamic range")和高灵敏度及特异性(参见"Tighter control of polymerase activity yields greater specificity")。

此外,与常规方法获得的分离曲线相比,使用RT2 SYBR Green FAST Mastermixes得到的分离曲线有更低的背景和更明显的曲线(参见"Sharper dissociation curves with RT2 SYBR Green Fast Mastermix")。

Principle

RT2 SYBR Green FAST Mastermixes包含real-time PCR缓冲液、高性能的HotStart DNA Taq聚合酶、核苷酸和SYBR Green染料。有些预混液包含fluorescein荧光素或ROX染料,用于优化real-time PCR分析仪的光学元件。化学修饰并严格控制的HotStart酶可避免扩增引物二聚体和其他非特异性产物,提供准确的SYBR Green结果。RT2 SYBR Green FAST Mastermixes经优化后使用10s变性和30s退火/延伸的PCR实验方案,而不影响特异性和灵敏度。

不含ROX或荧光素的预混液

RT2 SYBR Green FAST Mastermix非常适合在不需要参比染料的仪器上进行基于SYBR Green的real-time PCR应用,仪器包括Bio-Rad models CFX96、CFX384;Bio-Rad/MJ Research Chromo4、DNA Engine Opticon、DNA Engine Opticon 2;Roche LightCycler 480(96孔和384孔);无ROX滤过装置的Eppendorf Mastercycler ep realplex以及Cepheid SmartCycler。

含有荧光素的预混液

RT2 SYBR Green Fluor FAST Mastermix非常适合在需要fluorescein荧光素作为参比染料的仪器上进行基于SYBR Green的real-time PCR应用,仪器包括Bio-Rad models iCycler、iQ5、MyiQ和MyiQ2。

含有ROX的预混液 

RT2 SYBR Green ROX FAST Mastermix非常适合在需要ROX作为参比染料的仪器上进行基于SYBR Green的real-time PCR应用,仪器包括QIAGEN的Rotor-Gene Q实时荧光定量PCR分析仪;Applied Biosystems models 5700、7000、7300、7500(标准和快速)、7700、7900HT(标准和快速的96-well block,384-well block)、StepOnePlus和ViiA 7(标准和快速的96-well block,384-well block);含有或无ROX滤过装置的Eppendorf Mastercycler ep realplex;Stratagene models Mx3000P、Mx3005P、Mx4000以及Takara TP-800。

Applications
RT2 SYBR Green FAST Mastermixes适合用于real-time PCR分析。
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