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RT2 Profiler PCR Arrays

用于可靠、灵敏的基因表达谱分析

  • 检测一组基因的表达谱
  • 简单的real-time PCR方法具有高灵敏度
  • 只需1 ng总RNA
  • 可在各种real-time PCR分析仪上使用
  • 使用免费的在线工具进行便利的数据分析
RT2 Profiler PCR Arrays是经优化的real-time PCR引物,可在96孔板、384孔板或100孔盘上,用于对通路或疾病相关的基因进行分析,并含有相应的RNA质量控制。RT2 Profiler PCR Arrays进行的基因表达分析具有real-time PCR的灵敏度和微阵列的多基因检测能力。
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产品 Product no. 货号 目录价:
 
 
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RT2 Profiler PCR Arrays适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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Positive results with as little as 25 ng RNA.
Different amounts of universal total RNA were characterized using the Human Inflammatory Cytokines and Receptors RT2 Profiler PCR Array and the percentage of detectable genes calculated for each amount. As little as 25 ng total RNA yields a >80% positive call, even for cytokines expressed at very low levels.
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Simple procedure.
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A single gene-specific product in every reaction.

Universal total RNA was characterized using the TGFβ/BMP Signaling Pathway RT2 Profiler PCR Array, followed by dissociation (melting) curve analysis. The PCR array specifically detects individual genes, despite the expression of related gene-family members in the same RNA sample.

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PCR arrays yield highly accurate results.
A representative set of assays for 4000 genes used in RT2 Profiler PCR Arrays have an average amplification efficiency of 99% with a 95% CI from 90–110%. Consistently high amplification efficiencies enable PCR arrays to accurately analyze multiple genes simultaneously utilizing the ΔΔCT method.
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High reproducibility among different users.
4 replicate sets of raw threshold data (1–4) obtained by 2 different scientists (A and B) at 2 different times using Human Drug Metabolism RT2 Profiler PCR Arrays were directly compared. The results demonstrate a high degree of correlation (R2 >0.990).
Performance
灵敏度

RT2 First Strand Kit灵敏度高,每个芯片使用至少1 ng或至多5 µg总RNA可获得高于80%的阳性信号率(参见"Positive results with as little as 25 ng RNA")。

重复性

整个PCR芯片体系与技术上的重复性、批次和仪器有很高的相关性,平均相关系数>0.99,确保对生物样本间的表达差异进行可靠的检测(参见"High reproducibility among different users")。

特异性

PCR芯片体系使用高品质RNA,可在预期位置形成单条带,无引物二聚体或其他二级产物,由此可获得高度准确的real-time PCR结果(参见"A single gene-specific product in every reaction")。

统一的PCR扩增效率

对多种样本的多个基因进行准确的基因表达比较,需要PCR芯片技术具有统一的PCR扩增效率。将专有的引物设计法则与严格的引物分析检测相结合,确保PCR芯片的每次引物分析都具有高性能(参见"PCR arrays yield highly accurate results")。

 

 

Principle

RT2 Profiler PCR Arrays是分析一组特定基因表达的可靠工具。每个96孔板、384孔板或100孔盘PCR芯片都包含SYBR® Green优化的引物分析,可对相关的通路或疾病相关的基因进行细致的研究。RT2 Profiler PCR Arrays也可针对您特定的研究兴趣对一些基因作定制化分析。高品质引物设计和RT2 SYBR® Green qPCR Mastermix规格使PCR芯片可在统一的循环条件下同时扩增96或384个不同的基因特异性产物。

这种整合使RT2 Profiler PCR Array具有高特异性和扩增效率,可获得准确的SYBR® Green real-time PCR结果。PCR芯片在任何研究实验室都易于使用。

RT2 Profiler PCR Arrays具有足够高的敏感度,可使用从常规样本(0.1–5 µg RNA)、FFPE样本和小样本(1–100 ng RNA)制备的RNA。

Procedure

只需将cDNA模板与合适的即用型PCR预混液混合,等体积加入到同一个孔板的每个孔中,然后即可运行real-time PCR循环程序(参见"Simple procedure")。RT2 Profiler PCR Arrays可与所有QIAGEN、ABI、Bio-Rad、Eppendorf、Roche和Stratagene的仪器兼容。

灵活的布局和对照

RT2 Profiler PCR Arrays有96孔板、384孔板和100孔盘等规格,可检测84或370个与疾病或通路相关的基因,外加5个管家基因。每个RT2 Profiler PCR Array还包括以下对照因素:

数据标准化
基因组DNA污染检测
RNA样品质量 
总体PCR性能
易用的数据分析

可使用易用的基于Excel的数据分析模板或基于网络的软件进行数据分析。数据分析基于ΔΔCT法,原始数据可对每个管家基因标准化。

Applications

RT2 PCR Profiler Arrays可用于生物学和医学研究的各个领域,包括:

癌症研究
炎症和细胞因子分析
干细胞研究
神经生物学
信号转导通路研究
细胞黏附和细胞迁移
生物标记分子筛选和验证

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试剂盒操作手册 (4)

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For cDNA synthesis from 96 samples
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For pathway-focused gene expression profiling using real-time RT-PCR
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For quality control analysis prior to gene expression profiling using real-time RT-PCR
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仪器技术参数 (2)
For pathway-focused gene expression analysis
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For gene expression and genomic analysis
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参考文献
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