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qBiomarker Somatic Mutation PCR Assays

用于检测癌症和肿瘤发生时出现的特定DNA序列突变

  • 简单的real-time PCR操作流程
  • 灵敏度高,动态范围宽
  • 可在多数定量PCR仪上常规使用
  • 含有预混液

qBiomarker Somatic Mutation PCR Assays可用于检测细胞系或研究样本的个体特异性序列突变,对于毒理学、药物研发和癌症研究都十分重要。突变是从全面的体细胞突变数据库(如COSMIC)和科学文献中筛选,这些文献基于临床或功能相关性和发生频率。

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You can search for the following terms:
  • Entrez Gene IDs (e.g., 835)
  • RefSeq IDs (e.g., NM_032983, NP_116765)
  • Gene symbols (e.g., CASP2)
  • Cat. no. (e.g., SI00299551, QT01342509)
  • Sanger ID or Accession (e.g., hsa-let-7b, MI0000063)
  • CpG loci identification numbers (CG#) (e.g., CG17753661)

Do not enter species information in the Search box. Use the drop-down list to select your species of interest.
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产品 Product no. 货号 目录价:
qBiomarker Somatic Mutation PCR Assays
PCR assay and master mix
337011 Varies Enter your targets in search box above.

qBiomarker Somatic Mutation PCR Assays适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


Principle
Real-time PCR是检测DNA突变的灵敏、可靠地方法。通过将等位基因特异性扩增和水解探针检测相结合,qBiomarker Somatic Mutation real-time PCR assays可用于检测在野生型基因组DNA背景下至少1%的体细胞突变。Amplification Refractory Mutation System (ARMS)技术可进行等位基因特异性扩增,这种技术基于Taq聚合酶对PCR引物3’端匹配与错配的识别(参见"Principle of ARMS technology")。
Procedure

从新鲜、冷冻或固定样本中提取出基因组DNA后,将每个样本等量加入到含有合适预混液和qBiomarker Somatic Mutation PCR Assay的real-time PCR管中。然后,将等量样本加入到含有预混液和相应参考基因拷贝分析的real-time PCR管中。最后,运行推荐的循环程序即可。

运用仪器上的软件确定每个突变特异性分析和相应参考基因拷贝分析的CT值。然后,将数值粘贴到正确的Excel数据分析模板,以确定哪个样本含有检测的突变。

Applications

qBiomarker Somatic Mutation PCR Assays非常适合快速、准确的鉴定新鲜、冷冻或固定样本的个体特异性序列突变。

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常见问题 (20)
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仪器技术参数 (2)
For screening disease-focused mutation panels by PCR
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For gene expression and genomic analysis
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试剂盒操作手册 (1)
For real-time PCR-based, pathway- or disease-focused, somatic mutation profiling
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