qBiomarker Somatic Mutation PCR Arrays

用于快速、准确地分析体细胞DNA的突变状态

分析通路或疾病相关的体细胞的突变状态
简单的real-time PCR操作流程
灵敏度高，动态范围宽
可在多数PCR仪上常规使用
含有预混液

qBiomarker Somatic Mutation PCR Arrays是快速、准确分析体细胞突变状态的研究工具，可分析生物通路或疾病相关的重要基因。突变是从全面的体细胞突变数据库（如COSMIC）和科学文献中筛选，这些文献基于临床或功能相关性和发生频率。

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产品	Product no.	货号	目录价：
qBiomarker Somatic Mutation PCR Array for EGFR Pathway PCR plate and master mix show details	337021	SMH-001AA	varies	configure
qBiomarker Somatic Mutation PCR Array for ErbB2 Pathway PCR plate and master mix show details	337021	SMH-002AA	varies	configure
qBiomarker Somatic Mutation PCR Array for FLT3 Pathway PCR plate and master mix show details	337021	SMH-003AA	varies	configure
qBiomarker Somatic Mutation PCR Array for PDGFR Pathway PCR plate and master mix show details	337021	SMH-004AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human FGFR Pathway PCR plate and master mix show details	337021	SMH-005AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human KIT Pathway PCR plate and master mix show details	337021	SMH-006AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human c-MET Pathway PCR plate and master mix show details	337021	SMH-007AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Receptor Tyrosine Kinase (RTK) Pathways (Panel I) PCR plate and master mix show details	337021	SMH-008AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Receptor Tyrosine Kinase (RTK) Pathways (Panel Il) PCR plate and master mix show details	337021	SMH-009AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human APC/CTNNB1 (beta-catenin) Pathway PCR plate and master mix show details	337021	SMH-010AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human p53/Rb Pathway PCR plate and master mix show details	337021	SMH-011AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human RAS-RAF Pathway PCR plate and master mix show details	337021	SMH-013AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human PI3K-AKT Signaling Pathway PCR plate and master mix show details	337021	SMH-014AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Breast Cancer PCR plate and master mix show details	337021	SMH-020AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Colon Cancer PCR plate and master mix show details	337021	SMH-021AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Hematopoietic Neoplasms PCR plate and master mix show details	337021	SMH-022AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Lung Cancer PCR plate and master mix show details	337021	SMH-023AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Skin Cancers PCR plate and master mix show details	337021	SMH-024AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Brain Cancers PCR plate and master mix show details	337021	SMH-025AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Lymphoid Neoplasms PCR plate and master mix show details	337021	SMH-026AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Soft Tissue Tumors PCR plate and master mix show details	337021	SMH-027AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Thyroid Cancer PCR plate and master mix show details	337021	SMH-028AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Melanoma PCR plate and master mix show details	337021	SMH-029AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Bladder Cancer PCR plate and master mix show details	337021	SMH-030AA	varies	configure
qBiomarker™qBiomarker™ Somatic Mutation PCR Array Human Endometrial Cancer PCR plate and master mix show details	337021	SMH-032AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Endometrial Cancer PCR plate and master mix show details	337021	SMH-032BA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Kidney Cancer PCR plate and master mix show details	337021	SMH-033BA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Kidney Cancer PCR plate and master mix show details	337021	SMH-033CA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Liver Cancer PCR plate and master mix show details	337021	SMH-034AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Pancreatic Cancer PCR plate and master mix show details	337021	SMH-035AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Prostate Cancer PCR plate and master mix show details	337021	SMH-036BA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Head & Neck Cancer PCR plate and master mix show details	337021	SMH-037AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Esophageal Cancer PCR plate and master mix show details	337021	SMH-038AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Ovarian Cancer PCR plate and master mix show details	337021	SMH-039AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Gastric Cancer PCR plate and master mix show details	337021	SMH-041AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Acute Myeloid Leukemia PCR plate and master mix show details	337021	SMH-045AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Myelodysplastic Syndromes PCR plate and master mix show details	337021	SMH-046AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Oncogene Panel (384-well format) PCR plate and master mix show details	337021	SMH-3002AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Oncogene and Tumor Suppressor Panel (384-well format) PCR plate and master mix show details	337021	SMH-3003AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Tumor Suppressor Panel (384-well format) PCR plate and master mix show details	337021	SMH-3004AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Tyrosine Kinases and Cell Signaling Pathways (384-well format) PCR plate and master mix show details	337021	SMH-3005AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Lung and Colon Cancers (Expanded Panel) PCR plate and master mix show details	337021	SMH-3006AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Cancer Comprehensive 384HC PCR plate and master mix show details	337021	SMH-3007AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Tumor Suppressor Genes 384HC PCR plate and master mix show details	337021	SMH-3008AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human Receptor Tyrosine Kinase Signaling 384HC PCR plate and master mix show details	337021	SMH-3009AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human APC Gene PCR plate and master mix show details	337021	SMH-801AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human BRAF Gene PCR plate and master mix show details	337021	SMH-802AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human CTNNB1 Gene PCR plate and master mix show details	337021	SMH-803AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human EGFR Gene PCR plate and master mix show details	337021	SMH-804AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human KIT Gene PCR plate and master mix show details	337021	SMH-805AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human KRAS Gene PCR plate and master mix show details	337021	SMH-806AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human PDGFR Gene PCR plate and master mix show details	337021	SMH-807AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human PIK3CA Gene PCR plate and master mix show details	337021	SMH-808AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human PTEN Gene PCR plate and master mix show details	337021	SMH-809AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human RUNX1 Gene PCR plate and master mix show details	337021	SMH-810AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human STK11 Gene PCR plate and master mix show details	337021	SMH-811AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human TP53 Gene PCR plate and master mix show details	337021	SMH-812AA	varies	configure
qBiomarker™ Somatic Mutation PCR Array Human DNA QC Pathway PCR plate and master mix show details	337021	SMH-999AA	varies	configure

qBiomarker Somatic Mutation PCR Arrays适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。

Performance

qBiomarker Somatic Mutation PCR Arrays具有很高的灵敏性，甚至能检测福尔马林固定、石蜡包埋（FFPE）的样本（参见"High degree of sensitivity"和"Sensitivity of qBiomarker Somatic Mutation PCR Arrays with FFPE samples"）。

qBiomarker Somatic Mutation PCR Arrays可用于检测细胞系或研究样本的突变，对于毒理学、药物研发和癌症研究都十分重要（参见"Profiling of common cancer cell lines for somatic mutation status"）。

Principle

Real-time PCR是检测DNA突变的灵敏、可靠的方法。通过将等位基因特异性扩增和水解探针检测相结合，qBiomarker Somatic Mutation real-time PCR assays可用于检测在野生型基因组DNA背景下低至1%的体细胞突变。Amplification Refractory Mutation System (ARMS)技术可进行等位基因特异性扩增，这种技术基于Taq聚合酶对PCR引物3’端匹配与错配的识别（参见"Principle of ARMS technology"）。

Procedure

将DNA模板和即用型PCR预混液简单混合，等体积加入到相同孔板的各个孔中，然后运行real-time PCR循环程序即可。qBiomarker Somatic Mutation PCR Arrays能与所有的ABI、Bio-Rad、Eppendorf、Roche和Stratagene仪器兼容。

qBiomarker Somatic Mutation PCR Arrays有96孔板和384孔板2种规格，可用于检测疾病或通路相关的突变，而且含有可用于标准化基因拷贝数的对照。qBiomarker Somatic Mutation PCR Array也包含常规PCR的对照因子。

易于使用的数据分析

可使用Excel数据分析模板对数据进行分析。数据分析基于∆∆C_T法或Average ∆C_T法。

qBiomarker Somatic Mutation PCR Array有以下规格，每种规格已包括预混液：

qBiomarker Somatic Mutation PCR Array Format A: Fluoroscein, 96-well; for Bio-Rad iCycler, iQ5, MyiQ, and MyiQ2 instruments

qBiomarker Somatic Mutation PCR Array Format A: ROX, 96-well; for ABI Standard 96-well Blocks (5700, 7000, 7300, 7500, 7900HT, ViiA 7); Bio-Rad Chromo 4 (MJ Research); Stratagene Mx3005p, Mx3000p; Eppendorf ep realplex 2/2S, and 4/4S instruments

qBiomarker Somatic Mutation PCR Array Format C: ROX, 96-well; for ABI 7500 FAST 96-well Block, 7900HT FAST 96-Well Block, StepOnePlus, and ViiA 7 FAST 96-well Block instruments

qBiomarker Somatic Mutation PCR Array Format D: ROX, 96-well; for Bio-Rad CFX96, Opticon and Opticon 2 (MJ Research); Stratagene Mx4000 instruments

qBiomarker Somatic Mutation PCR Array Format E: ROX, 384-well; for ABI 7900HT 384-well Block, ViiA 7 384-well Block; Bio-Rad CFX384 instruments

qBiomarker Somatic Mutation PCR Array Format F: ROX, 96-well; for Roche LightCycler 480 96-well Block instruments

qBiomarker Somatic Mutation PCR Array Format G : ROX, 384-well; for Roche LightCycler 480 96-well Block instruments

Applications

qBiomarker Somatic Mutation PCR Arrays非常适合对通路或疾病相关的基因以及重要的下游信号基因进行快速、准确的突变分析。

应用说明 (1)

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	High-quality genomic DNA isolation and sensitive mutation analysis	显示更多

常见问题 (20)

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	How many different genes and gene mutations are on a Somatic Mutation PCR Array? FAQ ID -2427	浏览
	How are the different mutations for a single gene normalized on the qBiomarker Somatic Mutation PCR array? FAQ ID -2428	浏览
	Where are the somatic mutations published? FAQ ID -2429	浏览
	How can I check DNA sample quality before using the Somatic Mutation PCR Arrays & Assays? FAQ ID -2430	浏览
	How much DNA starting sample is needed for analysis with a Somatic Mutations PCR Array? And Assay? FAQ ID -2431	浏览
	What is the sensitivity of the assays contained on the qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2910	浏览
	How many mutations can I profile per sample on the qBiomarker Somatic Mutation PCR Array? FAQ ID -2911	浏览
	Were all assays on the qBiomarker Somatic Mutation PCR Arrays bench-validated? FAQ ID -2912	浏览
	How do you decide on the qBiomarker Somatic Mutation PCR Arrays to include on the pathway arrays and disease arrays? FAQ ID -2913	浏览
	What are the control features on the qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2914	浏览
	What is the data analysis method, and where can I find it for the qBiomarker Somatic Mutatation PCR arrays? FAQ ID -2915	浏览
	What is the principle for qBiomarker Somatic Mutation PCR Array data analysis? FAQ ID -2916	浏览
	What PCR machine do I need to use to run such a qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2917	浏览
	What effects can be predicted with poor sample quality? How robust is the platform with questionable sample quality (such as FFPE samples)? FAQ ID -2918	浏览
	What sample types can I test on the qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2919	浏览
	What method(s) do you recommend as an alternative way to validate the mutations identified on the somatic mutation PCR arrays? FAQ ID -2920	浏览
	What are the advantages of RT-PCR based somatic mutation PCR arrays and assays compared to other platforms? FAQ ID -2921	浏览
	What pathways and diseases are currently covered for the qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2922	浏览
	How do I use the qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2923	浏览
	What are the technological principles behind somatic mutation PCR arrays and assays? FAQ ID -2924	浏览

文章 (1)

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	(EN) Somatic mutations and copy number changes in cancer: finding the right targetsresponse	浏览

白皮书 (1)

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	(EN) - Rapid and accurate cancer somatic mutation profiling with the qBiomarker Somatic Mutation PCR Arrays	显示更多

仪器技术参数 (2)

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	(EN) - qBiomarker Somatic Mutation PCR Arrays For screening disease-focused mutation panels by PCR	显示更多
	QIAGEN Service Core - (EN) For gene expression and genomic analysis	显示更多

试剂盒操作手册 (1)

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	(EN) - qBiomarker Somatic Mutation PCR Handbook For real-time PCR-based, pathway- or disease-focused, somatic mutation profiling	显示更多

补充实验方案 (7)

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	Applied Biosystems 7500 (Fast block) real-time PCR run setup instructions for qBiomarker Somatic Mutation PCR Arrays	显示更多
	Applied Biosystems 7900HT real-time PCR run setup instructions for qBiomarker Somatic Mutation PCR Arrays	显示更多
	Bio-Rad iCycler and iQ real-time PCR run setup instructions for qBiomarker Somatic Mutation PCR Arrays	显示更多
	Rotor-Gene Q real-time PCR setup instructions for qBiomarker Somatic Mutation PCR Arrays	显示更多
	Roche LightCycler 480 real-time PCR run setup instructions for qBiomarker Somatic Mutation PCR Arrays	显示更多
	Stratagene Mx3000P QPCR System real-time PCR run setup instructions for qBiomarker Somatic Mutation PCR Arrays	显示更多
	Applied Biosystems ViiA7 real-time PCR run setup instructions for qBiomarker Somatic Mutation PCR Arrays	显示更多

安全数据表 (1)

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	MSDS qBiomarker Somatic Mutation PCR Arrays

参考文献