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qBiomarker Somatic Mutation PCR Arrays

用于快速、准确地分析体细胞DNA的突变状态

  • 分析通路或疾病相关的体细胞的突变状态
  • 简单的real-time PCR操作流程
  • 灵敏度高,动态范围宽
  • 可在多数PCR仪上常规使用
  • 含有预混液

qBiomarker Somatic Mutation PCR Arrays是快速、准确分析体细胞突变状态的研究工具,可分析生物通路或疾病相关的重要基因。突变是从全面的体细胞突变数据库(如COSMIC)和科学文献中筛选,这些文献基于临床或功能相关性和发生频率。

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qBiomarker Somatic Mutation PCR Arrays适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


Performance

qBiomarker Somatic Mutation PCR Arrays具有很高的灵敏性,甚至能检测福尔马林固定、石蜡包埋(FFPE)的样本(参见"High degree of sensitivity"和"Sensitivity of qBiomarker Somatic Mutation PCR Arrays with FFPE samples")。

qBiomarker Somatic Mutation PCR Arrays可用于检测细胞系或研究样本的突变,对于毒理学、药物研发和癌症研究都十分重要(参见"Profiling of common cancer cell lines for somatic mutation status")。

Principle
Real-time PCR是检测DNA突变的灵敏、可靠的方法。通过将等位基因特异性扩增和水解探针检测相结合,qBiomarker Somatic Mutation real-time PCR assays可用于检测在野生型基因组DNA背景下低至1%的体细胞突变。Amplification Refractory Mutation System (ARMS)技术可进行等位基因特异性扩增,这种技术基于Taq聚合酶对PCR引物3’端匹配与错配的识别(参见"Principle of ARMS technology")。
Procedure

将DNA模板和即用型PCR预混液简单混合,等体积加入到相同孔板的各个孔中,然后运行real-time PCR循环程序即可。qBiomarker Somatic Mutation PCR Arrays能与所有的ABI、Bio-Rad、Eppendorf、Roche和Stratagene仪器兼容。

qBiomarker Somatic Mutation PCR Arrays有96孔板和384孔板2种规格,可用于检测疾病或通路相关的突变,而且含有可用于标准化基因拷贝数的对照。qBiomarker Somatic Mutation PCR Array也包含常规PCR的对照因子。

易于使用的数据分析

可使用Excel数据分析模板对数据进行分析。数据分析基于∆∆CT法或Average ∆CT法。

qBiomarker Somatic Mutation PCR Array有以下规格,每种规格已包括预混液:

qBiomarker Somatic Mutation PCR Array Format A: Fluoroscein, 96-well; for Bio-Rad iCycler, iQ5, MyiQ, and MyiQ2 instruments
qBiomarker Somatic Mutation PCR Array Format A: ROX, 96-well; for ABI Standard 96-well Blocks (5700, 7000, 7300, 7500, 7900HT, ViiA 7); Bio-Rad Chromo 4 (MJ Research); Stratagene Mx3005p, Mx3000p; Eppendorf ep realplex 2/2S, and 4/4S instruments
qBiomarker Somatic Mutation PCR Array Format C: ROX, 96-well; for ABI 7500 FAST 96-well Block, 7900HT FAST 96-Well Block, StepOnePlus, and ViiA 7 FAST 96-well Block instruments
qBiomarker Somatic Mutation PCR Array Format D: ROX, 96-well; for Bio-Rad CFX96, Opticon and Opticon 2 (MJ Research); Stratagene Mx4000 instruments
qBiomarker Somatic Mutation PCR Array Format E: ROX, 384-well; for ABI 7900HT 384-well Block, ViiA 7 384-well Block; Bio-Rad CFX384 instruments
qBiomarker Somatic Mutation PCR Array Format F: ROX, 96-well; for Roche LightCycler 480 96-well Block instruments
qBiomarker Somatic Mutation PCR Array Format G : ROX, 384-well; for Roche LightCycler 480 96-well Block instruments
Applications

qBiomarker Somatic Mutation PCR Arrays非常适合对通路或疾病相关的基因以及重要的下游信号基因进行快速、准确的突变分析。

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仪器技术参数 (2)
For screening disease-focused mutation panels by PCR
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For gene expression and genomic analysis
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试剂盒操作手册 (1)
For real-time PCR-based, pathway- or disease-focused, somatic mutation profiling
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参考文献
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