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Microbial DNA qPCR Multi-Assay Kits

用于real-time PCR方法的微生物鉴定或分析
  • 微生物检测分析的完整试剂盒,包含所需的引物、探针、对照和试剂
  • 包含经多个实验验证的Microbial DNA qPCR Assay
  • 采用5'水解探针进行高度灵敏的特异性检测 
Microbial DNA qPCR Multi-Assay Kits是real-time PCR微生物检测的完整解决方案。Microbial DNA qPCR Multi-Assay Kits含有多个Microbial DNA qPCR Assay,后者含有两种PCR引物(每种10 µM)、以及一种5'水解探针(5 µM)用于定量real-time PCR。该试剂盒还含有成功进行qPCR所需的所有试剂和对照,包括Microbial DNA Positive Control、Positive PCR Control、Microbial DNA-Free Water和Microbial qPCR Mastermix。
产品 Product no. 货号 目录价:
 
 
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Available Products
28
产品 Product no. 货号 目录价:
One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1501Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1502Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1503Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1504Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1505Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1506Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1507Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1508Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1509Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1510Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1511Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1512Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1513Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1514Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1515Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1516Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1517Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1518Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1519Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1520Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1521Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1522Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1523Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1524Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1525Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1526Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1551Z
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One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each)
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330043 BBID-1552Z
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Configure
产品 Product no. 货号 目录价:
 
 
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Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.

Microbial DNA qPCR Multi-Assay Kits适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


Performance
最低定量阈限(LLOQ)
LLOQ是指在标准曲线线性范围内的最低模板浓度(参见Limit of detection versus lower limit of quantification)。所有Microbial DNA qPCR Assays中的93%具有低至100个基因拷贝的LLOQ(参见The LLOQ for all Microbial DNA qPCR Assays reveals high sensitivity)。该系列产品中的92%微生物检测产品能达到这一LLOQ,95%的毒力因子检测产品和97%的抗生素耐药性基因检测产品能达到这一LLOQ(参见The LLOQ for microbial identification Microbial DNA qPCR Assays reveals high sensitivityThe LLOQ for virulence factor genes Microbial DNA qPCR Assays reveals high sensitivityThe LLOQ for antibiotic resistance genes Microbial DNA qPCR Assays reveals high sensitivity)。

特异性
所有Microbial DNA qPCR Assay都经过严格检验,确保能够灵敏检测某一物种或基因(参见Microbial DNA qPCR Assays are highly specific)。对于能够检测一种以上五种或基因的产品,产品说明页上附有电脑预测可检测靶标的列表。

即便样本中存在多种物种,该试剂盒仍有极高的检测特异性,此类样本包括粪便、痰和菌斑(参见Microbial DNA qPCR Assays display high sensitivity even in complex metagenomic samples)。
Principle
Microbial DNA qPCR Assays在进行物种检测时,可检测细菌的16S rRNA基因和真菌核糖体rRNA基因序列;采用PCR扩增引物和水解探针,检测毒力因子基因和抗生素耐药性基因。
Procedure
Microbial DNA qPCR Multi-Assay Kit实验流程简单,能够在各种real-time PCR仪上使用。使用相应的QIAamp试剂盒从样本中分离DNA,然后使用与PCR仪相应的Microbial qPCR Mastermix构建PCR反应体系。每种样本进行三个对照反应,包括Positive PCR Control、No Template Control和Microbial DNA Positive Control、Microbial DNA qPCR Assays。进行real-time PCR,然后使用在线数据分析软件或Excel模板文件进行数据分析。
Applications
Microbial DNA qPCR Multi-Assay Kits高度适用于检测多种样本中相关的细菌或真菌物种,或微生物的抗生素耐药性基因或毒力因子基因。

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产品介绍与指南 (2)
Microbial product configurator guide
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For application-specific microbial identification and profiling by real-time PCR
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常见问题 (19)
FAQ ID — 3393
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FAQ ID — 3394
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FAQ ID - 3535
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试剂盒操作手册 (1)
New version – For real-time PCR-based profiling/detection of microbial species, antibiotic resistance genes or virulence factor genes
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