dNTP Set and dNTP Mix, PCR Grade
- 单独的dNTP或者即用型预混液
- 高纯度
- 适合于普通的和高灵敏度的PCR
dNTP Set, PCR Grade是含高纯度dNTP的组合装。每种dNTP为100 mM,以水溶液的形式提供,可与其他dNTP混合达到所需的浓度。dNTP Mix, PCR Grade含有预混的dATP、dCTP、dGTP和dTTP各10 mM,溶于水溶液中(pH 7.5)。因为PCR反应中存在的任何污染物都会导致PCR扩增灵敏度和产率下降,因此高纯度dNTP对于反应的成功非常重要。
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dNTP Mix, PCR Grade (200 µl)
Mix containing 10 mM each of dATP, dCTP, dGTP, and dTTP (1 x 200 µl)
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201900
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dNTP Mix, PCR Grade (800 µl)
Mix containing 10 mM each of dATP, dCTP, dGTP, and dTTP (4 x 200 µl)
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201901
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dNTP Set, PCR Grade, 4 x 100 µl
100 mM each dATP, dCTP, dGTP, dTTP for 1000 x 50 µl PCR reactions
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201912
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dNTP Set, PCR Grade, 4 x 250 µl
100 mM each dATP, dCTP, dGTP, dTTP for 2500 x 50 µl PCR reactions
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201913
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dNTP Set and dNTP Mix, PCR Grade are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Performance
每个批次的dNTP Set and Mix, PCR Grade都经过需四种核苷酸进行的特异PCR反应验证。
Principle
PCR反应中存在的任何污染物都会导致PCR扩增灵敏度和产率下降,因此高纯度dNTPs对于反应的成功非常关键。由于dNTPs纯度高,dNTP Set and dNTP Mix高度适用于敏感的PCR。
Procedure
针对dNTP Set and Mix的实验方案可在相应的QIAGEN PCR Kit中找到。相应的试剂盒操作手册可从我们的网站下载。
Applications
dNTP Set and dNTP Mix, PCR Grade适用于多种常规和敏感的PCR。另外,本产品可以与各种PCR酶或者RT-PCR酶配合使用,包括Taq DNA Polymerase、HotStarTaq DNA Polymerase和逆转录酶。
dNTP Set and dNTP Mix, PCR Grade可用于:
- 标准、单细胞、长片段及高保真PCR
- 基因分型
- 甲基化特异性PCR(MSP)
- 两步法RT-PCR
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